Organization of cytoskeleton during differentiation of gravisensitive root sites under clinorotation.

Key role in cell gravisensing is attributed to the actin cytoskeleton which acts as a mediator in signaling reactions, including graviperception. Despite of increased attention to the actin cytoskeleton, major gaps in our understanding of its functioning in plant gravisensing still remain. To fill these gaps, we propose a novel approach focused on the investigation of actin involvement in the development of columella cells and cells in the transition zone of roots submitted to clinorotation. Both statocytes and cells in the transition zone represent the postmitotic cells which take origin in root meristems and are specified into graviperceptive (root cap) and gravireacting (transition zone) root tissues. The aim of the research was to investigate and compare the microfilament arrangements in root cap statocytes and peripheral root tissues (epidermis and cortex cells) in the transition zone and to find out how the actin cytoskeleton is involved in their specification under clinostat conditions. So far, our experiments have shown that under clinorotation the cytoplasmic microfilament network in the cortex cells in the transition zone is significantly enhanced. It is suggested that more abundant cytoplasmic microfilaments could strengthen the cortical actin cytoskeleton arranged parallel with the cortical microtubules, which are found to be partially disorganized in this area. Due to microtubule disorganization, the functioning of cellulose-synthesizing machinery and proper deposition of cell wall might be affected and could cause the alterations in the growth mode. But, in our case growth of the cells in the transition zone under clinorotation was rather stable. Due to our opinion, general stability of cell growth under clinorotation is promoted by mutual functional interrelation between actin and tubulin cytoskeletons. It is suggested that a strengthened cortical actin cytoskeleton restricts the cell growth instead of disorganized microtubules.     

Neuritogenesis: a model for space radiation effects on the central nervous system.

Pivotal to the astronauts' functional integrity and survival during long space flights are the strategies to deal with space radiations. The majority of the cellular studies in this area emphasize simple endpoints such as growth related events which, although useful to understand the nature of primary cell injury, have poor predictive value for extrapolation to more complex tissues such as the central nervous system (CNS). In order to assess the radiation damage on neural cell populations, we developed an in vitro model in which neuronal differentiation, neurite extension, and synaptogenesis occur under controlled conditions. The model exploits chick embryo neural explants to study the effects of radiations on neuritogenesis. In addition, neurobiological problems associated with long-term space flights are discussed.     

Satellite observations of upper atmosphere O3 and HNO3 from the Limb Infrared Monitor of the Stratosphere (LIMS) experiment on Nimbus 7

The Limb Infrared Monitor of the Stratosphere (LIMS) experiment is a limb scanning infrared sounder designed to measure vertical temperature profiles and the concentrations of key chemical compounds which are important in the stratospheric ozone-nitrogen photochemistry. This paper describes results from the O₃ and HNO₃ channels with emphasis on validation of the data. Similar discussions of results from the other channels are presented in two companion papers published in these proceedings.     

FIGARO: An experiment for pulsar and variable source studies in the MeV range

We present a large area, balloon borne, NaI(Tl) detector for low-energy gamma rays with temporal signature : FIGARO.The main detector is a mosaic of 12 NaI(Tl) tiles 22.5 × 15 × 5 cm, for a total geometric area of 4050 cm².In the energy band 140 keV - 6 MeV, the expected background counting rate at float altitude is in the range of two to three thousands counts per second.For pulsar analysis the expected 3δ sensitivity for 5 hours exposition time is 2.5 10^?4 ph/cm².s.MeV (150–500 keV) 1.5 10^?4 ph/cm².s.MeV (1–6 MeV). This performance, together with the large effective area and the relatively short duration of a balloon flight, make FIGARO particularly suitable for the identification of sources by means of temporal analysis.For objectives in the Northern sky, including the Crab pulsar, a transmediterranean flight is planned for the summer of 1982 ; a Southern mission is scheduled in Brazil for the fall of 1983 (Vela, PSR 1822-09).     

Expression of cloned genes of transgenic microorganisms introduced into man-made ecosystems.

Modeling of transgenic microorganism introduction into small man-made ecosystems can help forecast changes in expression of cloned genes under different conditions of existence. Introduction of the E. coli Z905/pPHL7 strain containing a plasmid with luminescent system genes of luminous bacteria led to changes in cell and colony morphology, reduction in metabolic activity of cells, and, as a result, a lower level of expression of cloned gene. A low concentration of nutrients has been shown to favor greatly the phenotypic change of cells of the recombinant strain. Expression of cloned genes changed due to: a lower concentration of plasmid DNA, a change in regulation of cloned genes, and a change in cells of biosynthesis of substrates needed for expression of luminescent genes. The conducted investigations can provide a basis for the use of marker transgenic microorganisms in closed ecosystems of different types. Grant numbers: 99-04-96017, 00-07-9011.     

Problems in the analysis of gamma-ray burst spectra

Spectral measurements by the Solar Maximum Mission have been used to confirm the cyclotron lines in gamma-ray bursts reported from the Konus experiment. We present ISEE-3 data for the same burst (GB800419) during the same period of time which show no line. We discuss various problems in the analysis of scintillator spectra and point out that unfolded spectra are not necessarily unique and that the position of a data point in a deconvolved spectrum may vary depending on the assumed overall shape of the spectrum. As a result, if the analysis assumes a soft spectrum (such as optically thin thermal bremsstrahlung) an absorption feature might appear, whereas a harder spectrum (such as a Comptonized blackbody) would not require the feature. Since the continuum shape probably changes during the duration of a typical burst, the nonuniqueness of the spectral unfolding, combined with the assumption that the continuum is optically thin thermal bremsstrahlung, could give rise to spurious “absorption” features which vary on a time scale of seconds. Despite these problems, there is still some evidence for $\text{narrow}$ spectral lines in the range 45 to 65 keV but not for the broad lines reported from the Konus experiment. Unfortunately, the range 45 to 65 keV is the most difficult spectral region to unfold.     

Highly variable X-ray emitting objects in the Rho Oph dark cloud

The Rho Ophiuchi dark cloud region has been the subject of an extensive guest investigation using the $\text{Einstein}$ Observatory. The set of observations comprise 14 IPC fields and 3 HRI fields. The densest part of the cloud has been observed 6 times. Forty seven sources were detected at a level > 3.5 σ and twenty more above 2 σ. The majority of these sources have optical, IR, or even radio continuum counterparts; nine are identified with known T Tauri stars, while several others are identified with stars showing H α in emission. All show a high degree of time variability; flux variations reach factors of 5 in a few hours, or 25 in a day. Apparent luminosities are in the range 10(30) – 10(31)(1) erg.s^?1. The possibility that the X-ray variability is due to flares is examined. If this interpretation is correct, one source has been the seat of the largest stellar flare ever recorded in X rays [L_x = 10(32) erg.s^?1, E_x ?10(36) ergs^-].     

Radar auroral observations and ionospheric electric fields

The ground-based systems STARE and SABRE utilize radar auroral phenomena to estimate ionospheric electric fields. Some of the assumptions underlying these systems have been tested and general agreement with expectations have been found. However, as the results have been analysed in detail, it has become clear that the error in the irregularity drift velocity can at times amount to 100 ms^?1. Direct comparisons with other E-field measurements, as well as assessments of the results of applications of the Stare data clearly demonstrate that the electric field, calculated on the basis of the irregularity drift velocity, is a useful estimate of the actual horizontal electric field in the ionosphere and is sufficiently accurate for a great variety of geophysical studies.     

Signatures of troposphere-stratosphere momentum coupling: Implications for global atmospheric angular momentum and earth rotation budgets

During January–August 1978, the global atmospheric angular momentum (M) exhibits distinct patterns of short term momentum interchange across latitudes. In the northern hemisphere winter-spring season, 30–50 day modulations of M are present in which momentum $\text{enhancements}$ at mid-latitudes (20–30°) are closely matched by momentum $\text{depressions}$ at high latitudes (50–60°). During the same interval there are no corresponding variations in M evident in the southern hemisphere. Conversely, during southern hemisphere fall-winter, similar anticorrelations in monthly scale momentum excursions are evident between mid and high latitudes. In the northern hemisphere, the winter-spring momentum signatures are detected throughout the atmosphere, from the lower troposphere to the stratosphere. During the southern hemisphere fall-winter, the modulation patterns are not evident at the higher altitudes. Structural details of the momentum signatures indicate that the coupling is sometimes effective on very short time scales, e.g. 1–2 days, or less. The evidence of distinct anti-correlation between large regions has interesting implications for studies of global atmospheric circulation, and also for studies of the excitation of variations in earth rotation in response to short term modulations of M.