Interaction of growth-determining systems with gravity

The experiments have been carried out with lettuce shoots on board the Salyut-7 orbital station, the Kosmos-1667 biological satellite and under ground conditions at 180° plant inversion. By means of the centrifuge Biogravistat-1M the threshold value of gravitational sensitivity of lettuce shoots has been determined on board the Salyut-7 station. It was found to be equal to 2.9 × 10⁻³g for hypocotyls and 1.5 × 10⁻⁴g for roots. The following results have been received in the experiment performed on board the Kosmos-1667 satellite: a) under microgravity the proliferation of the meristem cells and the growth of roots did not differ from the control; b) the growth of hypocotyls in length was significantly enhanced in microgravity; c) under microgravity transverse growth of hypocotyls (increase in cross sectional area) was significantly increased due to enhancement of cortical parenchyma cell growth. At 180° inversion in Earth's gravity root extension growth and rate of cell division in the root apical meristem were decreased. The determination of DNA-fuchsin value in the nuclei of the cell root apexes showed that inversion affected processess of the cell cycle preceeding cytokinesis.     

Genetic response of bacterial spores to very heavy ions.

Using spores of two Bacillus subtilis strains differing in repair capacity, we have studied repair and mutation induction in the spores after irradiation with very heavy ions up to uranium with specific particle energies up to 18.6 MeV/u. The results indicate that repair and mutation induction after heavy ion irradiation are closely related to each other and that both phenomena strongly depend on the atomic number and specific energy of the ions. The effects are discussed in comparison with results obtained after X-irradiation.     

ERA-experiment "Space Biochemistry".

The general goal of the experiment was to study the response of anhydrobiotic (metabolically dormant) microorganisms (spores of Bacillus subtilis, cells of Deinococcus radiodurans, conidia of Aspergillus species) and cellular constituents (plasmid DNA, proteins, purple membranes, amino acids, urea) to the extremely dehydrating conditions of open space, in some cases in combination with irradiation by solar UV-light. Methods of investigation included viability tests, analysis of DNA damages (strand breaks, DNA-protein cross-links) and analysis of chemical effects by spectroscopic, electrophoretic and chromatographic methods. The decrease in viability of the microorganisms was as expected from simulation experiments in the laboratory. Accordingly, it could be correlated with the increase in DNA damages. The purple membranes, amino acids and urea were not measurably effected by the dehydrating condition of open space (in the dark). Plasmid DNA, however, suffered a significant amount of strand breaks under these conditions. The response of these biomolecules to high fluences of short wavelength solar UV-light is very complex. Only a brief survey can be given in this paper. The data on the relatively good survival of some of the microorganisms call for strict observance of COSPAR Planetary Protection Regulations during interplanetary space missions.     

Engineering strategies for the design of plant nutrient delivery systems for use in space: approaches to countering microbiological contamination.

Microbiological contamination of crops within space-based plant growth research chambers has been postulated as a potentially significant problem. Microbial infestations; fouling of Nutrient Delivery System (NDS) fluid loops; and the formation of biofilms have been suggested as the most obvious and important manifestations of the problem. Strict sanitation and quarantine procedures will reduce, but not eliminate, microbial species introduced into plant growth systems in space habitats. Microorganisms transported into space most likely will occur as surface contaminants on spacecraft components, equipment, the crew, and plant-propagative materials. Illustrations of the potential magnitude of the microbiological contamination issue will be drawn from the literature and from documentation of laboratory and commercial field experience. Engineering strategies for limiting contamination and for the development of countermeasures will be described. Microbiological control technologies and NDS hardware will be discussed. Configurations appropriate for microgravity research facilities, as well as anticipated bio-regenerative life support system implementations, will be explored. An efficiently designed NDS, capable of adequately meeting the environmental needs of crop plants in space, is considered to be critical in both the research and operational domains. Recommended experiments, tests, and technology developments, structured to allow the development of prudent engineering solutions also will be presented.     

The impact of alternative mission models on the future orbital debris environment

In reviewing discussions of future directions for space activity, it becomes obvious that there are a large number of groups formulating a wide diversity of plans for the future use of space. These plan alternatives are being made to account for user needs, technology development constraints, economic constraints, and launch support, and each of the plans will have direct or indirect effects on the orbital debris environment in terms of mass to orbit, deposition of operational debris, and control of accidental breakups. Thus it is important to develop the ability to project future debris states for a range of possible space traffic scenarios. The impact that these possible traffic environments would have on space operations forms the basis for studies of alternative options for the usage of space. In this paper, the effects on the orbital debris environment of a base-line mission model and two alternatives are investigated, using a numerical debris environment simulation code under development at JSC.     

Productivity and food value of Amaranthus cruentus under non-lethal salt stress.

Stress effects from the accumulation of metal salts may pose a problem for plants in closed biological systems such as spacecraft. This work examined the effects of salinity on growth, photosynthesis and carbon allocation in the crop plant, Amaranthus. Plants were germinated and grown in modified Hoagland's solution with NaCl concentrations of 0 to 1.0%. Plants received salt treatments at various times in development to assess effects on particular life history phases. For Amaranthus cruentus, germination, vegetative growth, flowering, seed development and yield were normal at salinities from 0 to 0.2%. Inhibition of these phases increased from 0.2 to 0.4% salinity and was total above 0.5%. 1.0% salinity was lethal to all developmental phases. Onset of growth phases were not affected by salinity. Plants could not be adapted by gradually increasing salinity over days or weeks. Water uptake increased, while photosynthetic CO2 uptake decreased with increasing salinity on a dry weight basis during vegetative growth. Respiration was not affected by salinity. After flowering, respiration and photosynthesis decreased markedly, such that 1.0% NaCl inhibited photosynthesis completely. Protein levels were unchanged with increasing salinity. Leaf starch levels were lower at salinities of 0.5% and above, while stem starch levels were not affected by these salinities. The evidence supports salt inhibition arising from changes in primary biochemical processes rather than from effects on water relations. While not addressing the toxic effects of specific ions, it suggests that moderate salinity per se need not be a problem in space systems.     

User issues and Earth observation data policy

The second workshop of the EOPOLE project was held in Cosenza, Italy, 10–12 May 1999, organised by Microwave Consultant Engineering (MiCE). Its purpose was to address the user issues related to Earth observation data policy. This report presents the main conclusions of the workshop. The different approaches among lead departments within national governments emerged as one of the main areas of which users in Europe need to be aware.     

Need and effect of reconditioning of nickel/hydrogen batteries

There has been a debate about the need for reconditioning nickel/hydrogen batteries in geosynchronous satellites. A study was done as part of life cycling, to determine the necessity of reconditioning and its effect on the cell performance. A 36 Ah nickel/hydrogen cell was put on a GEO simulated cycling at 15°C without reconditioning up to four eclipse seasons. The effect of reconditioning on the fifth and sixth eclipse seasons was studied. The study has conclusively proven the need for reconditioning and has shown the benefits of a high rate reconditioning. It has also been possible to draw some conclusions about the effect of a long duration trickle charge on the positive electrode     

Raindrops on Titan.

Some of the aspects of methane precipitation on Titan are considered. In particular, descent velocities are computed. It is found that raindrops fall much slower than on Earth. Additionally, the maximum size of raindrops on Titan is over 9 mm, compared with under 6 mm on Earth. The composition of drops will vary with altitude. Implications of these properties for Titan and the Huygens mission are considered.