Repair of DNA double-strand breaks and cell killing by charged particles.

It has been suggested that it is not simple double-strand breaks (dsb) but the non-reparable breaks which correlate well with the high biological effectiveness of high LET radiations for cell killing (Kelland et al., 1988; Radford, 1986). We have compared the effects of charged particles on cell death in 3 pairs of cell lines which are normal or defective in the repair of DNA dsbs. For the cell lines SL3-147, M10, and SX10 which are deficient in DNA dsb repair, RBE values were close to unity for cell killing induced by charged particles with linear energy transfer (LET) up to 200 keV/micrometer and were even smaller than unity for the LET region greater than 300 keV/micrometer. The inactivation cross section (ICS) increased with LET for all 3 pairs. The ICS of dsb repair deficient mutants was always larger than that of their parents for all the LET ranges, but with increasing LET the difference in ICS between the mutant and its parent became smaller. Since a small difference in ICS remained at LET of about 300 keV/micrometer, dsb repair may still take place at this high LET, even if its role is apparently small. These results suggest that the DNA repair system does not play a major role in protection against the attack of high LET radiations and that a main muse of cell death is non-reparable dsb which are produced at a higher yield compared with low LET radiations. No correlation was observed between DNA content or nuclear area and ICS.     

Development of a plant growth unit for growing plants over a long-term life cycle under microgravity conditions.

To study the effect of the space environment on plant growth including the reproductive growth and genetic aberration for a long-term plant life cycle, we have initiated development of a new type of facility for growing plants under microgravity conditions. The facility is constructed with subsystems for controlling environmental elements. In this paper, the concept of the facility design is outlined. Subsystems controlling air temperature, humidity, CO2 concentration, light and air circulation around plants and delivering recycled water and nutrients to roots are the major concerns. Plant experiments for developing the facility and future plant experiments with the completed facility are also overviewed. We intend to install this facility in the Japan Experiment Facility (JEM) boarded on the International Space Station.     

Modelling of genetically engineered microorganisms introduction in closed artificial microcosms.

The possibility of introducing genetically engineered microorganisms (GEM) into simple biotic cycles of laboratory water microcosms was investigated. The survival of the recombinant strain Escherichia coli Z905 (Apr, Lux+) in microcosms depends on the type of model ecosystems. During the absence of algae blooming in the model ecosystem, the part of plasmid-containing cells E. coli decreased fast, and the structure of the plasmid was also modified. In conditions of algae blooming (Ankistrodesmus sp.) an almost total maintenance of plasmid-containing cells was observed in E. coli population. A mathematics model of GEM's behavior in water ecosystems with different level of complexity has been formulated. Mechanisms causing the difference in luminescent exhibition of different species are discussed, and attempts are made to forecast the GEM's behavior in water ecosystems.     

Investigation of the motion of (99942) apophis asteroid using the SKIF cyberia multiprocessor computing system

We present the results of investigation of the dynamics of (99942) Apophis asteroid, which will undergo a very close encounter with the Earth on April 13, 2029. The region of possible motions of the asteroid is considered on the time interval (2004, 2040). In addition, it is shown that an increase of the observational interval (2004, 2006) until 2008 allowed us to reduce significantly the area of possible motions. All investigations were performed by numerical methods with the help of algorithms and software developed by us in the environment of parallel programming using the SKIF Cyberia multiprocessor computer of the Tomsk State University.     

National and Regional Grids

The title of this paper provides a point of departure for the broadest possible discussion of electric power transmission and presents an opportunity to comment on some misconceptions regarding power systems. The subject of transmission is discussed in terms of 1) the grid concept, 2) its system function, and 3) its probable future development and some of the problems facing such development.     

Small-Signal Model for the Series Resonant Converter

The results of a previous discrete-time model of the series resonant dc-dc converter are reviewed and from these a small signal dynamic model is derived. This model is valid for low frequencies and is based on the modulation of the diode conduction angle for control. The basic converter is modeled separately from its output filter to facilitate the use of these results for design purposes. Experimental results are presented.     

Goals and preparation method for ground operations for the manned space flight Altair

The mission's success fully depends on the Payload Operations conducted during the space flight. The Ground Team has to be trained to assist the Space Crew, to replan the cosmonaut's activities when contingengies occurr onboard and to change or cancel Payload activities when required. In order to act efficiently during the mission, the Ground Team must be prepared in advance of the flight and able to operate special tools for tracking the mission's progress, anticipating problems and taking decisions in realtime.

This document sets out the approach for conducting such a preparation for Ground Operation. It will be focused on the Altaïr mission performed in July 1993 onboard the Russian Mir space station.     

Aircraft vertical profile implementation using directed-graphmethods

Aircraft vertical profile simulation is realized using a demand-driven minimal-calculation directed graph structure to reduce calculation time and to force synchronization of the performance measurement functions with the system state variables. Performance-directed model adaptation makes dynamic vertical profile path corrections, in the presence of fixed drag variations, possible. Drag variations ranging from +10% to -10% yielded fuel consumption improvements of less than 1% in the majority of the cases. Calculation time improvement for path simulation ranges from a factor of 1.19 in the worst case to 1.5 in the best case     

Host–parasite interactions in closed and open microbial cultivation system

The study addresses interaction of bacteria and phages in the host–parasite system in batch and continuous cultures. The study system consists of the auxotrophic strain of Brevibacterium – Brevibacterium sp. 22L – and the bacteriophage of Brevibacterium sp., isolated from the soil by the enrichment method.

1.

Closed system. In the investigation of the relationship between the time of bacterial lysis and multiplicity of phage infection it has been found that at a lower phage amount per cell it takes a longer time for the lysis of the culture to become discernible. Another important factor determining cytolysis in liquid medium is the physiological state of bacterial population. Specific growth rate of bacteria at the moment of phage infection has been chosen as an indicator of the physiological state of bacteria. It has been shown that the shortest latent period and the largest output of the phage are observed during the logarithmic growth phase of bacteria grown under favorable nutrient conditions. In the stationary phase, bacterial cells become “a bad host” for the phage, whose reproduction rate decreases, and the lysis either slows down significantly or does not occur at all.