Long-term survival of bacterial spores in space.

On board of the NASA Long Duration Exposure Facility (LDEF), spores of Bacillus subtilis in monolayers (10(6)/sample) or multilayers (10(8)/sample) were exposed to the space environment for nearly six years and their survival was analyzed after retrieval. The response to space parameters, such as vacuum (10(-6) Pa), solar electromagnetic radiation up to the highly energetic vacuum-ultraviolet range (10(9) J/m2) and/or cosmic radiation (4.8 Gy), was studied and compared to the results of a simultaneously running ground control experiment. If shielded against solar ultraviolet (UV)-radiation, up to 80 % of spores in multilayers survive in space. Solar UV-radiation, being the most deleterious parameter of space, reduces survival by 4 orders of magnitude or more. However, up to 10(4) viable spores were still recovered, even in completely unprotected samples. Substances, such as glucose or buffer salts serve as chemical protectants. With this 6 year study in space, experimental data are provided to the discussion on the likelihood of "Panspermia".     

Surface tension-driven flows in horizontal liquid metal layers

The problem of surface tension-driven flows in horizontal liquid layers has been studied experimentally, and theoretically by direct numerical simulation and small perturbation analysis. We focus our attention on situations in which the depth of the fluid (liquid tin; small Prandtl number, Pr=0.015) is small enough to ensure the predominance of the surface tension forces over those due to the buoyancy. The surface velocity has been experimentally obtained for liquid tin layer with various aspect ratio (length to height) in the range 5<A<83. The thermal gradients are ranged from 5 to 40°K/cm. In the numerical study, the Navier-Stokes and energy equations are solved by an efficient finite difference technique. The parameters governing the flow behaviour in the liquid are varied to determine their effects on thermocapillary convection: the Reynolds number 10<Re<210⁴ and the aspect ratio 2<A<25; with Pr kept constant at Pr=0.015. The linear eigenequation resulting from small spatial disturbances of the Couette flow solution is solved using an Tau-Chebyshev approximation. A notable feature of the theoretical study is the totally different end circulations. In the region near the cold wall a multicell structure is evident. This agrees with the eigensolution which is of complex type, indicating spatial periodicity. In the hot wall region the flow is accelerated to reach the velocity value for the fully-developed Couette flow which is reached under conditions such as Re/A<20. The transition from viscous to boundary layer regime occurs for a critical value (Re/A)_c of nearly about 200, as deduced from the numerical and experimental results.     

Repair of DNA double-strand breaks and cell killing by charged particles.

It has been suggested that it is not simple double-strand breaks (dsb) but the non-reparable breaks which correlate well with the high biological effectiveness of high LET radiations for cell killing (Kelland et al., 1988; Radford, 1986). We have compared the effects of charged particles on cell death in 3 pairs of cell lines which are normal or defective in the repair of DNA dsbs. For the cell lines SL3-147, M10, and SX10 which are deficient in DNA dsb repair, RBE values were close to unity for cell killing induced by charged particles with linear energy transfer (LET) up to 200 keV/micrometer and were even smaller than unity for the LET region greater than 300 keV/micrometer. The inactivation cross section (ICS) increased with LET for all 3 pairs. The ICS of dsb repair deficient mutants was always larger than that of their parents for all the LET ranges, but with increasing LET the difference in ICS between the mutant and its parent became smaller. Since a small difference in ICS remained at LET of about 300 keV/micrometer, dsb repair may still take place at this high LET, even if its role is apparently small. These results suggest that the DNA repair system does not play a major role in protection against the attack of high LET radiations and that a main muse of cell death is non-reparable dsb which are produced at a higher yield compared with low LET radiations. No correlation was observed between DNA content or nuclear area and ICS.     

Development of a plant growth unit for growing plants over a long-term life cycle under microgravity conditions.

To study the effect of the space environment on plant growth including the reproductive growth and genetic aberration for a long-term plant life cycle, we have initiated development of a new type of facility for growing plants under microgravity conditions. The facility is constructed with subsystems for controlling environmental elements. In this paper, the concept of the facility design is outlined. Subsystems controlling air temperature, humidity, CO2 concentration, light and air circulation around plants and delivering recycled water and nutrients to roots are the major concerns. Plant experiments for developing the facility and future plant experiments with the completed facility are also overviewed. We intend to install this facility in the Japan Experiment Facility (JEM) boarded on the International Space Station.     

Computational fluid dynamics and material processing (buoyancy and thermocapillary convections in melts during directional crystallization)

Modelisation and solution of heat and mass transfer problems relevant for material processing are generally hard to be handled, as they often involve 3D unsteady flows, viscous mixtures, phase changes, moving liquid-solid fronts, deforming liquid-gas interfaces, etc.… For space applications, material processing benefits of reduced buoyancy convection but can be faced to a strongly increased complexity due to variable g, mainly in manned flight.

Computational techniques used to analyse fluid motions in material processing, accounting for free surface, crystallization front and bulk convection in melt, are reviewed with emphasis to directional crystallization. Hydrodynamics stability and bifurcation analysis are shown to be useful complementary tools for correlating data, and for a better understanding of the physical laws. This last point will be illustrated in the case of the onset of oscillations in metallic melts.     

A calibration neutron monitor: Statistical accuracy and environmental sensitivity

This paper is a follow-on of that of Krüger et al. [Krüger, H., Moraal, H., Bieber, J.W., Clem, J.M., Evenson, P.A., Pyle, K.R., Duldig, M.L., Humble, J.E. A calibration neutron monitor: energy response and instrumental temperature sensitivity. J. Geophys. Res. 113, A08101, doi:10.1029/2008JA013229, 2008], that describes the characteristics of a pair of calibration neutron monitors that were developed to intercalibrate the count rates of the world’s neutron monitors against each other. Such an intercalibration will allow the calculation of energy (rigidity) spectra, which will enhance the quality of the neutron monitor data. Krüger et al. (2008) investigated the energy and temperature response of the calibrators. This paper studies the statistical accuracy of the calibration procedure, its repeatability, and the sensitivity to its environment. The paper concludes with a calibration procedure that can minimise the uncertainties caused by these five effects, or at least correct for them.     

Mapping Regolith and Gossan for Mineral Exploration in the Eastern Kumaon Himalaya,India using hyperion data and object oriented image classification

Crystalline in the Kumaon Himalaya, India near Askot area is a prominent site of the base metal mineralization and gossanised surface. This area is hosted by the sulphides and sulphates of Cu, Pb, Zn and Au and Ag mineralization with the altered rocks like sericite chlorite schist, gneiss etc. Due to the deep weathering this area is also a good illustration site of the gossanised outcrop.     

Charge exchange lifetimes for ring current ions

In applying the charge exchange mechanism to ion phenomena within the Earth's magnetosphere it is critical to the proper interpretation of observations that the charge exchange lifetimes for the ions be known as accurately as possible. Various new results have been published which significantly modify the charge exchange lifetimes which have been used in space physics research during the past decade and a half. Some of the newer results have been used in the application of the charge exchange decay mechanism but the use has been limited and for the most part incomplete. The neutral hydrogen density distribution now yields lifetimes which are shorter than previously calculated, while the functional dependence of the lifetimes on pitch angle provides for slower decay for ions mirroring off the geomagnetic equator. This review coalesces and summarizes the latest and best measurements of the physical quantities involved in the complete calculation of the charge exchange lifetime of the mirroring magnetospheric ions.     

Position-Location Solutions by Taylor-Series Estimation

Taylor-series estimation gives a least-sum-squared-error solution to a set of simultaneous linearized algebraic equations. This method is useful in solving multimeasurement mixed-mode position-location problems typical of many navigational applications. While convergence is not proved, examples show that most problems do converge to the correct solution from reasonable initial guesses. The method also provides the statistical spread of the solution errors.     

An optimum filter for direct A/D conversion

The author describes a design procedure to find the optimum finite-impulse-response (FIR) filter coefficients when the characteristics of the bandpass filter preceding the analog-to-digital (A/D) converter are known. A simulation of direct A/D conversion performance shows the improvement over the filter coefficients designed to minimize the maximum error over a specified band of frequencies. DC cancellation may be added to the process with very little change in the coefficients of the optimum FIR filter or its performance.<>