Signal transduction in T lymphocytes--a comparison of the data from space, the free fall machine and the random positioning machine.

In this paper we discuss the effect of microgravity on T cells and we present the data of studies with two new machines for 0 g simulations. Several experiments in space show that mitogenic T cell activation is lost at 0 g. Immunocytochemistry indicates that such effect is associated with changes of the cytoskeleton. Biochemical studies suggest that the lack of expression of the interleukin-2 receptor is one of the major causes of the loss of activity. In fact, interleukin-2 is the third signal required for full activation. In order to deepen our investigations we are now working with the free-fall machine, FFM, invented by D. Mesland, and with the random positioning machine, RPM, or three-dimensional clinostat, developed by T. Hoson. The FFM produces periods of free-fall lasting approximately 800 ms followed by bounces of 15-30 g lasting 45-60 ms. The RPM eliminates the effect of gravity by rotating biological specimen randomly around two orthogonal axes. While the FFM failed to reproduce the results obtained with T lymphocytes in space, the data from the RPM are in good agreement with those in real microgravity. In fact, the inhibition of the mitotic index in the RPM is 89% compared to static controls. The RPM (as the FFM) can carry markedly larger specimen than the fast rotating clinostat and thus allows to conduct comprehensive studies to select suitable biological objects for further investigations in space.     

The principle of constructing a computation model of equilibrium ribbed stiffened shells in linear displacement-based FEM analysis

The equilibrium between the rods and panels in the ribbed shells is considered based on the total system of structural mechanics equations as applied to a discrete model. Also shown is an approach to solving the problems that permits the stiffness matrix of shear panels to be obtained with regard for the equilibrium along the boundary with rods. The connection with the standard hybrid method of constructing stiffness matrices is indicated. The solutions for separate elements and examples illustrating the approach being proposed are presented.     

High temperature alters the growth reaction of Pottia protonemata.

Under gravistimulation, dark-grown protonemata of Pottia intermedia revealed negative gravitropism with a growth rate of approximately 28 μm·h⁻¹ at room temperature (20 °C). In 7 days, the protonema formed a bundle of vertically oriented filaments. At an elevated temperature (30 °C), bundles of vertically growing filaments were also formed. However, both filament growth rate and amplitude of the gravicurvature were reduced. Red light (RL) irradiation induced a positive phototropism of most apical protonemal cells at 20 °C. In a following period of darkness, approximately two-thirds of such cells began to grow upward again, recovering their negative gravitropism. RL irradiation at the elevated temperature caused a partial increase in the number of protonemal cells with negative phototropism, but the protonemata did not exhibit negative gravitropism after transfer to darkness. The negative gravitropic reaction was renewed only when protonemata were placed at 20 °C. A dramatic decrease in starch amount in protonemal apical cells, which are sensitive to both gravity and light, occurred at the higher temperature. Such a decrease may be one of the reasons for the inhibition of the protonemal gravireaction at the higher temperature. The observation has a bearing on the starch-statolith theory.     

The importance of charged aerosols in the polar mesosphere in connection with Noctilucent Clouds and Polar Mesosphere Summer Echoes

A number of campaigns have been conducted in order to study Polar Mesosphere Summer Echos (PMSE) and Noctilucent Clouds (NLC) in the period 1991–1994. Several sounding rockets have been launched through these layers with measurements being performed on upleg as well as downleg. These include measurements of positive ions and electrons in both ram and wake positions, as well as measurements of charged aerosols in ram on upleg. In this paper we will review these measurements and make a preliminary classification of the data based upon the presence of PMSE and/or NLC. One of the mechanisms responsible for PMSE is the presence of neutral air turbulence in combination with a high Schmidt number. We will briefly discuss this type of echo using in situ rocket data. Differences and similarities of PMSE and NLC as observed both in the Arctic and the Antarctic will be discussed. Observations show that especially PMSE are much more frequent in the Arctic. This may be due to a difference in the water vapour content or the temperature at mesopause heights. Lack of data in the Antarctic makes it difficult to decide which of these two factors are the most important. More measurements, especially co-ordinated in situ and ground-based lidar and radar measurements, are needed to discuss the Arctic and Antarctic similarities and differences in further detail.     

Modelling of genetically engineered microorganisms introduction in closed artificial microcosms.

The possibility of introducing genetically engineered microorganisms (GEM) into simple biotic cycles of laboratory water microcosms was investigated. The survival of the recombinant strain Escherichia coli Z905 (Apr, Lux+) in microcosms depends on the type of model ecosystems. During the absence of algae blooming in the model ecosystem, the part of plasmid-containing cells E. coli decreased fast, and the structure of the plasmid was also modified. In conditions of algae blooming (Ankistrodesmus sp.) an almost total maintenance of plasmid-containing cells was observed in E. coli population. A mathematics model of GEM's behavior in water ecosystems with different level of complexity has been formulated. Mechanisms causing the difference in luminescent exhibition of different species are discussed, and attempts are made to forecast the GEM's behavior in water ecosystems.     

Approaches in the determination of plant nutrient uptake and distribution in space flight conditions.

The effective growth and development of vascular plants rely on the adequate availability of water and nutrients. Inefficiency in either the initial absorption, transportation, or distribution of these elements are factors which impinge on plant structure and metabolic integrity. The potential effect of space flight and microgravity conditions on the efficiency of these processes is unclear. Limitations in the available quantity of space-grown plant material and the sensitivity of routine analytical techniques have made an evaluation of these processes impractical. However, the recent introduction of new plant cultivating methodologies supporting the application of radionuclide elements and subsequent autoradiography techniques provides a highly sensitive investigative approach amenable to space flight studies. Experiments involving the use of gel based 'nutrient packs' and the radionuclides calcium-45 and iron-59 were conducted on the Shuttle mission STS-94. Uptake rates of the radionuclides between ground and flight plant material appeared comparable.     

Effects of Local Detachment of a Melt from Ampoule Walls,Crystal Vibration,and Magnetic Field at Crystal Growth under Zero Gravity

A numerical investigation of the melt flow and heat and mass transfer is carried out at the crystal growth under zero gravity, when the melt detachment from ampoule walls, crystal vibration, and various magnetic fields are active. Specific features of the melt flow are demonstrated depending on the size of a detachment zone adjacent to the crystallization boundary. The velocity of the averaged flow generated by crystal vibration is determined as a function of the vibration intensity. It is shown that the crystal vibration cannot compensate a thermal capillary flow (caused by detachment of the melt from the ampoule wall) and reduce the macrosegregation of impurities. It is shown that the application of steady and rotating magnetic fields are inefficient for all ampoule methods of crystal growth under microgravity conditions.     

Coordinated Continual Planning Methods for Cooperating Rovers

Eight evaluation metrics are used to compare and contrast three coordination schemes for a system that continuously plans to control collections of rovers (or spacecraft) using collective mission goals instead of goals or command sequences for each spacecraft. These schemes use a central coordinator to either: 1) micromanage rovers one activity at a time; 2) assign mission goals to rovers; or 3) arbitrate mission goal auctions among rovers. A self-commanding collection of rovers would autonomously coordinate itself to satisfy high-level science and engineering goals in a changing partially understood environment - making the operation of tens or even a hundred spacecraft feasible     

Mean winds of the mesosphere and lower thermosphere (60–110 km): A global distribution from radar systems (MF,Meteor, VHF)

During the last decade a large number of radars (～12) have been developed, which have produced substantial quantities of tidally-corrected mean winds data. The distribution of the radars is not global, but many areas are well covered: the Americas with Poker Flat (65°N), Saskatoon (52°N), Durham (43°N), Atlanta (34°N), Puerto Rico (18°N); Europe with Kiruna (68°), Garchy (47°N) and Monpazier (44°N); and Oceania with Christchurch (44°S), Adelaide (35°S), Townsville (20°S), and Kyoto (35°N). Zonal and meridional wind height-time cross-sections from $\text{60}\text{80}$ km (MF/Meteor Radar) to ～110 km have been prepared for the last 5–6 years. They are compared with cross-sections from CIRA-72 for zonal winds, and Groves (1969) for meridional winds.It is shown that while CIRA-72 is still a useful model for many purposes, significant differences exist between it and the new radar data. The latter demonstrate important seasonal, latitudinal, longitudinal and hemispheric variations. The new meridional cross-sections are of great value. The common features with Groves (1969) are the equatorward cells in summer near 85 km; however their strength (～10 ms^?1) and size are less. Systematic and somewhat different variations emerge at higher (?52°N) and middle (35–44°) latitudes.     

A balloon-borne sun-tracking multichannel photometer for atmospheric aerosol measurements

A balloon borne multichannel photometer for measurement of atmospheric scattering in the near ultraviolet and the visible wavelength regions has been developed at the Physical Research Laboratory, Ahmedabad for study of the size distribution and number density of aerosols at tropospheric and lower stratospheric altitudes. The instrumentation involves tracking the sun in elevation and scanning in azimuth. The payload was recently flown on a 100 kg. balloon from the Hyderabad Balloon Facility on 18 April 1984. The balloon reached a float altitude of 35 km and good quality data has been obtained from an altitude of 6 km upto float altitude. Data analysis is still in progress. The present paper details the instrument design and presents a few illustrations of the instrument performance from this flight.