Spaceflight hardware allowing unilateral irradiation and chemical fixation in petri dishes.

To accommodate a spaceflight experiment with moss (SPM), experiment-unique equipment (EUE) was developed by engineers at Kennedy Space Center. The hardware allows sterile culture for an extended period of time in commercial petri dishes, lateral illumination of each culture with light of a specific wavelength (660 nm; other wavelengths are possible) and a range of intensities (0.05-5 micromoles photons m-2 s-1), incubation in complete darkness, and chemical fixation to terminate the experiment under conditions of microgravity. The use of a fixative required triple containment to protect the astronaut crew. An external panel on the experiment container allowed the timing of illumination and fixation to be controlled by the crew. Light quality is provided by light emitting diodes (LEDs) that are located in the lid of the outer container, the BRIC (Biological Research In Canisters)-LED. Each canister accommodates 6 Petri Dish Fixation Units (PDFUs), and each PDFU holds one 6 cm petri dish. All components are autoclavable. LED illumination is piped through a transparent glass rod. Each PDFU contains fixative in a reservoir that is released by the depression of an actuator. This hardware performed well during its first flight, the 16-day STS-87 mission in Nov./Dec., 1997 as part of the Collaborative USA and Ukrainian Experiment (CUE). It supported vigorous and sterile moss growth, cells were maintained in position and were well-fixed, and there was a vigorous and consistent response to light. Although here used for moss, in future flight experiments this unique new hardware can be used for many types of organisms normally grown in petri dishes, with or without a requirement for illumination.     

Robust altitude estimation for over-the-horizon radar using a state-space multipath fading model

In previous work, a matched-field estimate of aircraft altitude from multiple over-the-horizon (OTH) radar dwells was presented. This approach exploits the altitude dependence of direct and surface reflected returns off the aircraft and the relative phase changes of these micro-multipath arrivals across radar dwells. Since this previous approach assumed high dwell-to-dwell predictability, it has been found to be sensitive to mismatch between modeled versus observed micro-multipath phase and amplitude changes from dwell-to-dwell. A generalized matched-field altitude estimate is presented here based on a state-space model that accounts for random ionospheric and target-motion effects that degrade the dwell-to-dwell predictability of target returns. The new formulation results in an efficient, robust recursive maximum likelihood (ML) estimation of aircraft altitude. Simulations suggest that the proposed technique can achieve accuracy within 5,000 ft of the true aircraft altitude, even with relatively high levels of uncertainty in modeling of dwell-to-dwell changes in the target return. A real data result is also presented to illustrate the technique.     

Overview of the effects of neutral gas releases on high-voltage sounding rocket platforms

Four recent sounding rocket experiments demonstrate that the release of neutral gas from both positively and negatively charged vehicles returns the vehicle potential to near the plasma potential. Early active experiment payloads, CHARGE-2 and SPEAR-1, observed enhanced currents from/to the vehicles during unplanned attitude thruster firings. The follow-on CHARGE-2B and SPEAR-3 payloads directly measured the changes to the vehicle potential caused by both planned argon and unplanned nitrogen releases. The gas releases from positively charged CHARGE-2B consistently reduced the potential to 20–50 V, whereas the gas releases from negatively charged SPEAR-3 brought the potential to between −200 and −500 V. The difference in grounding levels for positively and negatively charged payloads suggests that the gas responsible for grounding is near the vehicle, within a small fraction of the sheath size.     

Gasdynamic lasers: review and extension

A review of the physics and gasdynamics associated with conventional CO₂-N₂ gasdynamic lasers (GDL's) is given, including a short survey of the state of the art. The role of advanced, downstream mixing GDL's is examined, and the question is addressed: Can such downstream mixing GDL's provide an order-of-magnitude increase in power output over the conventional device? Finally, combustion driven GDL's with unconventional fuels are examined, and new results for gain and maximum available power are given for various fuel-oxidizer combinations.     

Plasma wave observations during electron gun experiments on ISEE-1

The ISEE-1 electron guns were operated during the final orbits of ISEE-1 in 1987 in tests designed to study the stimulation of plasma waves. The guns were operated in modes which varied from 10-μA, at 10-eV, to 100-μA at 45-eV. Experiments were run on inbound orbits, while moving from the solar wind into perigee on the dusk side. A broadband emission was generally found from 0.1–10-kHz (e.g. below the plasma frequency). Next, a strong signal was typically induced at about 80-kHz, well above the ambient plasma frequency. This is interpreted as being the plasma frequency associated with the “beam” electrons. There were occasionally intensifications of the naturally occurring signals at the electron cyclotron frequency and the electron plasma frequency (or upper hybrid resonance).     

Germination and elongation of flax in microgravity.

This experiment was conducted as part of a risk mitigation payload aboard the Space Shuttle Atlantis on STS-101. The objectives were to test a newly developed water delivery system, and to determine the optimal combination of water volume and substrate for the imbibition and germination of flax (Linum usitatissimum) seeds in space. Two different combinations of germination paper were tested for their ability to absorb, distribute, and retain water in microgravity. A single layer of thick germination paper was compared with one layer of thin germination paper under a layer of thick paper. Paper strips were cut to fit snugly into seed cassettes, and seeds were glued to them with the micropyle ends pointing outward. Water was delivered in small increments that traveled through the paper via capillary action. Three water delivery volumes were tested, with the largest (480 microliters) outperforming the 400 microliters and 320 microliters volumes for percent germination (90.6%) and root growth (mean=4.1 mm) during the 34-hour spaceflight experiment. The ground control experiment yielded similar results, but with lower rates of germination (84.4%) and shorter root lengths (mean=2.8 mm). It is not clear if the roots emerged more quickly in microgravity and/or grew faster than the ground controls. The single layer of thick germination paper generally exhibited better overall growth than the two layered option. Significant seed position effects were observed in both the flight and ground control experiments. Overall, the design of the water delivery system, seed cassettes and the germination paper strip concept was validated as an effective method for promoting seed germination and root growth under microgravity conditions.     

The International Space Station human life sciences experiment implementation process.

The selection, definition, and development phases of a Life Sciences flight research experiment has been consistent throughout the past decade. The implementation process, however, has changed significantly within the past two years. This change is driven primarily by the shift from highly integrated, dedicated research missions on platforms with well defined processes to self contained experiments with stand alone operations on platforms which are being concurrently designed. For experiments manifested on the International Space Station (ISS) and/or on short duration missions, the more modular, streamlined, and independent the individual experiment is, the more likely it is to be successfully implemented before the ISS assembly is completed. During the assembly phase of the ISS, science operations are lower in priority than the construction of the station. After the station has been completed, it is expected that more resources will be available to perform research. The complexity of implementing investigations increases with the logistics needed to perform the experiment. Examples of logistics issues include- hardware unique to the experiment; large up and down mass and volume needs; access to crew and hardware during the ascent or descent phases; maintenance of hardware and supplies with a limited shelf life,- baseline data collection schedules with lengthy sessions or sessions close to the launch or landing; onboard stowage availability, particularly cold stowage; and extensive training where highly proficient skills must be maintained. As the ISS processes become better defined, experiment implementation will meet new challenges due to distributed management, on-orbit resource sharing, and adjustments to crew availability pre- and post-increment.