Changes in vacuolation in the root apex cells of soybean seedlings in microgravity.

Changes in the vacuolation in root apex cells of soybean (Glycine max L. [Merr.]) seedlings grown in microgravity were investigated. Spaceflight and ground control seedlings were grown in the absence or presence of KMnO4 (to remove ethylene) for 6 days. After landing, in order to study of cell ultrastructure and subcellular free calcium ion distribution, seedling root apices were fixed in 2.5% (w/v) glutaraldehyde in 0.1 M cacodylate buffer and 2% (w/v) glutaraldehyde, 2.5% (w/v) formaldehyde, 2% (w/v) potassium antimonate K[Sb(OH)6] in 0.1 M K2HPO4 buffer with an osmolarity (calculated theoretically) of 0.45 and 1.26 osmol. The concentrations of ethylene in all spaceflight canisters were significantly higher than in the ground control canisters. Seedling growth was reduced in the spaceflight-exposed plants. Additionally, the spaceflight-exposed plants exhibited progressive vacuolation in the root apex cells, particularly in the columella cells, to a greater degree than the ground controls. Plasmolysis was observed in columella cells of spaceflight roots fixed in solutions with relatively high osmolarity (1.26 osmol). The appearance of plasmolysis permitted the evaluation of the water status of cells. The water potential of the spaceflight cells was higher than the surrounding fixative solution. A decrease in osmotic potential and/or an increase in turgor potential may have induced increases in cell water potential. However, the plasmolysed (i.e. non-turgid) cells implied that increases in water potential were accompanied with a decrease in osmotic potential. In such cells changes in vacuolation may have been involved to maintain turgor pressure or may have been a result of intensification of other vacuolar functions like digestion and storage.     

Chemistry of the interstellar medium--an evolutionary dead end?

An analysis of the experimental data available and of the present theoretical concepts shows that even the initial physicochemical chemical precellular stages of biological evolution are impossible in the interstellar medium, while biomonomers possibly formed on asteroids and comets might have participated after transportation to the Earth in the final stages of the origin of the first precellular biological structures and then in the first living cells.     

An approach to the detection of microbe life in planetary environments through charge-coupled devices

The use of charge-coupled devices is suggested as a means for detecting growth of micro-organism colonies. The accuracy of the method is determined by channel width and the sensitivity by the signal/noise ratio. The method was tested on a dense nutrient medium, which is to be considered more suitable to micro-organisms of the dry Martian soil than the water solution of nutrients employed in the Viking's strategy.     

The Lightning And Radio Emission Detector (LRD) instrument

The Lightning and Radio Emission Detector (LRD) instrument will be carried by the Galileo Probe into Jupiter's atmosphere. The LRD will verify the existence of lightning in the atmosphere and will determine the details of many of its basic characteristics. The instrument, operated in its magnetospheric mode at distances of about 5, 4, 3, and 2 planetary radii from Jupiter's center, will also measure the radio frequency (RF) noise spectrum in Jupiter's magnetosphere. The LRD instrument is composed of a ferritecore radio frequency antenna ( 100 Hz to 100 kHz) and two photodiodes mounted behind individual fisheye lenses. The output of the RF antenna is analyzed both separately and in coincidence with the optical signals from the photodiodes. The RF antenna provides data both in the frequency domain (with three narrow-band channels, primarily for deducing the physical properties of distant lightning) and in the time domain with a priority scheme (primarily for determining from individual RF waveforms the physical properties of closeby-lightning).     

The Spacelab-Mir-1 "Greenhouse-2" experiment.

The Spacelab-Mir-1 (SLM-1) mission is the first docking of the Space Shuttle Atlantis (STS-71) with the Orbital Station Mir in June 1995. The SLM-1 "Greenhouse-2" experiment will utilize the Russian-Bulgarian-developed plant growth unit (Svet). "Greenhouse-2" will include two plantings (1) designed to test the capability of Svet to grow a crop of Superdwarf wheat from seed to seed, and (2) to provide green plant material for post-flight analysis. Protocols, procedures, and equipment for the experiment have been developed by the US-Russian science team. "Greenhouse-2" will also provide the first orbital test of a new Svet Instrumentation System (SIS) developed by Utah State University to provide near real time data on plant environmental parameters and gas-exchange rates. SIS supplements the Svet control and monitoring system with additional sensors for substrate moisture, air temperature, IR leaf temperature, light, oxygen, pressure, humidity, and carbon-dioxide. SIS provides the capability to monitor canopy transpiration and net assimilation of the plants growing in each vegetation unit (root zone) by enclosing the canopy in separate, retractable, ventilated leaf chambers. Six times during the seed-to-seed experiment, plant samples will be collected, leaf area measured, and plant parts fixed and/or dried for ground analysis. A second planting initiated 30 days before the arrival of a U.S. Shuttle [originally planned to be STS-71] is designed to provide green material at the vegetative development stage for ground analysis. [As this paper is being edited, the experiment has been delayed until after the arrival of STS-71.]     

Transition region line-shifts in the rebound shock spicule model

Spectral emission lines created in the solar chromosphere — corona transition region show net red-shifts. It has been proposed that this may be the result of the return of spicular material. We simulate a spicule numerically using the rebound shock model and find that the resulting hydrodynamic evolution leads to a perceived up-flow in transition region spectral lines even though the average velocity in the line forming region is directed downward. The explanation for this apparent paradox is found in the correlation between density and velocity in the waves generated by the rebound shock spicule.     

Radiation risk of the crew members of the expeditions on the "MIR" station during the 22nd solar activity cycle.

The radiation risk at the end of the flight was calculated for the members of the main expeditions on the "Mir" station. It was based on the absorbed dose dynamics data measured by the board dosimeter. The radiation damage models created for standards of the radiation safety of the space flights were used in the calculations. The analysis of the obtained values of the risk and its dynamics for some cosmonauts are presented in the topic. The risk values delta P are close to the limited levels given by equation of delta P = 0.6 x 10 x T(-4), [this equation appears also as delta RHrad = 0.6 x 10(-4) x T later in the text] where T--is flight duration in months.     

Induction of high grade astrocytoma (HGA) by protons: molecular mechanisms and RBE considerations.

Protons of a specific energy, 55 MeV, have been found to induce primary high grade astrocytomas (HGA) in the Rhesus monkey (Macaca mulatta). Brain tumors of this type were not induced by protons of other energies (32-2,300 MeV). Induction of HGA has been identified in human patients who have had radiation therapy to the head. We believe that the induction of HGA in the monkey is a consequence of dose distribution, not some unique "toxic" property of protons. Comparison of the human experience with the monkey data indicates the RBE for induction of brain tumors to be about one. It is unlikely that protons cause an unusual change in oncogenic expression, as compared to conventional electromagnetic radiation.     

Development of plant protoplasts during the IML-1 mission.

During the 8 day IML-1 mission, regeneration of cell walls and cell divisions in rapeseed protoplasts were studied using the Biorack microscope onboard the Space Shuttle "Discovery". Samples from microgravity and 1g protoplast cultures were loaded on microscope slides. Visual microscopic observations were reported by the payload specialist Roberta Bondar, by down-link video transmission and by use of a microscope camera. Protoplasts grown under microgravity conditions do regenerate cell walls but to a lesser extent than under 1g. Cell divisions are delayed under microgravity. Few cell aggregates with maximum 4-6 cells per aggregate are formed under microgravity conditions, indicating that microgravity may have a profound influence on plant cell differentiation.     

Nonlinear problems of physics of the geomagnetic pulsations

The current state of research involving manifestations of nonlinearity in geomagnetic pulsations is reviewed. Traditionally, the attention of researchers was focused on the effects of resonant interaction of geomagnetic pulsations with small groups of energetic particles, which actually means the study of the quasi-linear relaxation of radiation belt ions, the modulation of auroral electron fluxes, etc. The present review concentrates on the problem of the nonlinear effect influence of pulsations on the backgroud (cold) plasma and on the geomagnetic field. This kind of interaction results in a significant modification of the plasma distribution in the magnetosphere. Self-consistent wave structures—solitons and vortices may occur as well. Such nonlinear effects contribute to physics of geomagnetic pulsations and are also of fundamental importance for general physics. Another set of more narrow problems considered in the review, is related to phenomenological modeling of fluctuational and critical phenomena in the magnetosphere. The essence of our approach is to present the magnetosphere as a black box, whose properties should be determined by the statistical characteristics of its output signals. This approach to phenomenology can be a useful supplement to the methods of microscopic modeling aimed at detecting nonlinear manifestations of geomagnetic pulsations.