Bacterial community in ancient Siberian permafrost as characterized by culture and culture-independent methods

The microbial composition of ancient permafrost sediments from the Kolyma lowland of Northeast Eurasia was examined through culture and culture-independent approaches. These sediments have been continuously frozen for 5,000 to 2-3 million years. A total of 265 Bacteria 16S rRNA gene sequences were amplified from the permafrost total-community genomic DNA and screened by amplified ribosomal 16S rRNA restriction analysis. Members of three major lineages were found: gamma-Proteobacteria (mostly Xanthomonadaceae), Actinobacteria, and Firmicutes. We also determined partial 16S rRNA gene sequences of 49 isolates from a collection of 462 aerobes isolated from these sediments. The bacteria included Actinomycetales (Arthrobacter and Microbacteriaceae); followed by the Firmicutes (Exiguobacterium and Planomicrobium); the Bacteroidetes (Flavobacterium); the gamma-Proteobacteria (Psychrobacter); and the alpha-Proteobacteria (Sphingomonas). Both culture and culture-independent approaches showed the presence of high and low G+C Gram-positive bacteria and gamma-Proteobacteria. Some of the 16S rRNA gene sequences of environmental clones matched those of Arthrobacter isolates. Two-thirds of the isolates grew at -2.5 degrees C, indicating that they are psychroactive, and all are closely related to phylogenetic groups with strains from other cold environments, mostly commonly from Antarctica. The culturable and non-culturable microorganisms found in the terrestrial permafrost provide a prototype for possible life on the cryogenic planets of the Solar System.     

Spectral properties of Lake Superior banded iron formation: application to Martian hematite deposits

Several locations have been identified on Mars that expose bulk, coarsely crystalline gray hematite. These deposits have been interpreted as being sedimentary and formed in aqueous environments. Lake Superior Type (LST) banded iron formation (BIF) was investigated as a spectral and possible process analog to these deposits. In northern Michigan, LST BIF formed in a sedimentary, continental shelf or shallow basin environment under stable tectonic conditions, and the oxide facies contains gray, crystalline hematite. These deposits are Proterozoic in age and contain microfossils associated with the early diversification of life on Earth. Samples of the hematite-bearing oxide facies, as well as the carbonate facies, were collected and analyzed for their spectral and geochemical characteristics. Sample spectra were measured in the visible, near-infrared, and thermal infrared for comparison with remote and in situ spectra obtained at Mars. Thin section analysis, as well as X-ray diffraction and scanning electron microscopy measurements, were performed to determine detailed geochemistry. There is no evidence for BIF at Opportunity's Meridiani landing site, and the results of this work will provide useful data for determining whether BIFs exist elsewhere on Mars and are, thus, relevant to current and future Mars exploration missions.     

Doppler characteristics of HF spread Doppler clutter recorded at Alice Springs,Australia

Mean night-time peak power, Doppler shift and Doppler width of spread Doppler clutter (SDC) received by a high frequency backscatter radar located at Alice Springs, Australia from 2000 to 2018 is presented as a function of azimuth, sunspot number, time of year and frequency. The sampled region covers 90 degrees from West to North and includes the northern and southern equatorial anomalies.SDC peak power diminished across all azimuths during the winter solstice from around May to August (local winter) coinciding with the global decrease in F layer density due to the annual non-seasonal F2 anomaly but was generally constant during the equinoxes. In contrast, SDC Doppler width and inbound Doppler shift both increased during the equinoxes and exhibited azimuthal dependence related to the eastward equatorial plasma drift.SDC peak power increased with increasing sunspot number with frequency dependence during winter but not summer. Inbound Doppler shift and Doppler width increased with increasing sunspot number during equinox but not solstice with a strong dependence on azimuth and a weak dependence on frequency.     

Deorbiter CubeSat mission design

This paper presents the mission design for a CubeSat-based active debris removal approach intended for transferring sizable debris objects from low-Earth orbit to a deorbit altitude of 100 km. The mission consists of a mothership spacecraft that carries and deploys several debris-removing nanosatellites, called Deorbiter CubeSats. Each Deorbiter is designed based on the utilization of an eight-unit CubeSat form factor and commercially-available components with significant flight heritage. The mothership spacecraft delivers Deorbiter CubeSats to the vicinity of a predetermined target debris, through performing a long-range rendezvous maneuver. Through a formation flying maneuver, the mothership then performs in-situ measurements of debris shape and orbital state. Upon release from the mothership, each Deorbiter CubeSat proceeds to performing a rendezvous and attachment maneuver with a debris object. Once attached to the debris, the CubeSat performs a detumbling maneuver, by which the residual angular momentum of the CubeSat-debris system is dumped using Deorbiter’s onboard reaction wheels. After stabilizing the attitude motion of the combined Deorbiter-debris system, the CubeSat proceeds to performing a deorbiting maneuver, i.e., reducing system’s altitude so much so that the bodies disintegrate and burn up due to atmospheric drag, typically at around 100 km above the Earth surface. The attitude and orbital maneuvers that are planned for the mission are described, both for the mothership and Deorbiter CubeSat. The performance of each spacecraft during their operations is investigated, using the actual performance specifications of the onboard components. The viability of the proposed debris removal approach is discussed in light of the results.     

A novel satellite image encryption algorithm based on hyperchaotic systems and Josephus problem

With the rapid growth of the number of Earth observation satellite (EOS) supporting critical applications, it is required to improve the security techniques to protect the sensitive data and images during the transmission between the satellites and the ground stations. This paper introduces a new satellite image encryption algorithm based on the Linear Feedback Shift Register (LFSR) generator, SHA 512 hash function, hyperchaotic systems, and Josephus problem. LFSR generates a matrix that is used to construct the 512-bits value of the hash function. These bits are used to set the initial values and parameters of the proposed encryption algorithm. Firstly, the six dimensions (6-D) hyperchaotic system is divided into three parts, where every two equations are considered as one part. Secondly, the 1-D hyperchaotic logistic-tent system is considered as the controller to select one part. The selected part is used to generate a matrix that is XORed with the original image. Thirdly, the scrambling operation by Josephus sequences is applied to the output of the previous step by scrambling the rows and the columns according to the selected part to produce the pre-encrypted image. Finally, if the number of iterations is less than the required number which is considered as a parameter of the secret key, the previous operations will be repeated in the pre-encrypted image; otherwise, the pre-encrypted image is considered as the final cipher image. Experimental and analyses results show that the proposed algorithm has good performance in terms of high level of security, large enough key-space, tolerance to Single Event Upsets (SEU) as well as low time complexity.     

Effect of Thermal Treatment on CFRP Parts Before and After Adhesive Bonding

Numerous non-destructive techniques are being investigated for assuring quality of the adhesive bonds.The research presented here is focused on non-destructive assessment of carbon fibre reinforced polymer(CFRP)parts.The surface condition directly influences the performance of adhesive bonds.The structural joints should ensure safe usage of a structure.However,some modifications of the surface may lead to weak bond that cannot carry the desired load.This is why there is a search for methods of surface assessment before bonding.Moreover,reliable techniques are required to allow to verify the integrity of the adhesive bond after manufacturing or bonded repair.We focus on the laser induced fluorescence(LIF)method for assessing the surface state.The LIF is a noncontact measurement method.In the context of adhesive bond assessment the electromechanical impedance(EMI)method is studied.The EMI uses surface bonded piezoelectric sensors for excitation and sensing.The investigated samples were made of CFRP layers.The samples were treated at elevated temperatures.The influence of the thermal treatment was studied using LIF.The thermal treatment at 220℃could be clearly distinguishedrom the rest of the considered samples.The thermally treated plates were bonded to untreated plate and then they were measured with the EMI method to study the influence of the treatment on the adhesive bond.The changes of EMI spectra were significant for the treatment at 280 ℃ and for some thermally treated samples that were later contaminated with de-icing fluid.     

Numerical simulation of effects of the August 11, 1999 solar eclipse in the outer ionosphere

The results of a numerical simulation of such parameters of the topside ionosphere as concentration N _e and temperature T _e of electrons, and concentration n(H⁺) and fluxes along the magnetic field lines Φ(H⁺) of H⁺ ions at an altitude of ～2000 km for the conditions of the August 11, 1999 solar eclipse are presented. The calculations were performed using the Global Self-consistent Model of the Thermosphere, Ionosphere, and Protonosphere of the Earth (GSM TIP). It is shown that during the eclipse, in addition to a region of decreased values of T _e in the Northern Hemisphere and in the magnetically conjugate region in the Southern Hemisphere, regions of electron heating emerge in both hemispheres. Simultaneously, an extended region of decreased values of N _e comes into existence and moves behind the Moon’s shadow. Regions with decreased (down to ～30%) and enhanced (up to ～50%) concentrations of H⁺ ions are detected in the global distribution of these ions.