Immobilization induces a very rapid increase in osteoclast activity

We studied in a randomized, strictly controlled cross-over design, the effects of 6 days 6 degrees head-down tilt bed rest (HDT) in eight male healthy subjects in our metabolic ward. The study consisted of two periods (phases) of 11 days each in order to allow for the test subjects being their own controls. Both study phases were identical with respect to environmental conditions, study protocol and diet. Two days before arriving in the metabolic ward the subjects started with a diet. The diet was continued in the metabolic ward. The metabolic ward period (1l days) was divided into three parts: 4 ambulatory days, 6 days either HDT or control and 1 recovery day. Continuous urine collection started on the first day in the metabolic ward to analyze calcium excretion and bone resorption markers. On the 2nd ambulatory day in the metabolic ward and on the 5th day in HDT or control blood was drawn to analyze serum calcium, parathyroid hormone, and bone formation markers. Urinary calcium excretion was, as early as the first day in immobilization, increased (p<0.01). CTX- and NTX-excretion stayed unchanged in the first 24 h in HDT compared to the control. But already on the 2nd day of immobilization, both bone resorption markers significantly increased. We conclude from these results--pronounced rise of bone resorption markers--that already 24 h of immobilization induce a significant rise in osteoclast activity in healthy subjects. Thus, it appears possible to use short-term bed rest studies as a first step for the development of countermeasures to immobilization.     

Magnetic Field Fluctuations in the Solar Wind, Foreshock and Magnetosheath： Cluster Data Analysis

46 magnetosheath crossing events from the two years (2001.2-2003.1) of Cluster magnetic field measurements are identified and used to investigate the characters of the magnetic field fluctuations in the regions of undisturbed solar wind, foreshock, magnetosheath. The preliminary results indicate the properties of the plasma turbulence in the magnetosheath are strongly controlled by IMF orientation with respect to the bow shock normal. The amplitude of the magnetic field magnitude and direction variations behind quasi-parallel bow shock are larger than those behind quasi-perpendicular bow shock. Almost purely compressional waves are found in quasi-perpendicular magnetosheath.     

Setting a standard: the limulus amebocyte lysate assay and the assessment of microbial contamination on spacecraft surfaces

Historically, colony-forming units as determined by plate cultures have been the standard unit for microbiological analysis of environmental samples, medical diagnostics, and products for human use. However, the time and materials required make plate cultures expensive and potentially hazardous in the closed environments of future NASA missions aboard the International Space Station and missions to other Solar System targets. The Limulus Amebocyte Lysate (LAL) assay is an established method for ensuring the sterility and cleanliness of samples in the meat-packing and pharmaceutical industries. Each of these industries has verified numerical requirements for the correct interpretation of results from this assay. The LAL assay is a rapid, point-of-use, verified assay that has already been approved by NASA Planetary Protection as an alternate, molecular method for the examination of outbound spacecraft. We hypothesize that standards for molecular techniques, similar to those used by the pharmaceutical and meat-packing industries, need to be set by space agencies to ensure accurate data interpretation and subsequent decision making. In support of this idea, we present research that has been conducted to relate the LAL assay to plate cultures, and we recommend values obtained from these investigations that could assist in interpretation and analysis of data obtained from the LAL assay.     

The Mawrth Vallis region of Mars: A potential landing site for the Mars Science Laboratory (MSL) mission

The primary objective of NASA's Mars Science Laboratory (MSL) mission, which will launch in 2011, is to characterize the habitability of a site on Mars through detailed analyses of the composition and geological context of surface materials. Within the framework of established mission goals, we have evaluated the value of a possible landing site in the Mawrth Vallis region of Mars that is targeted directly on some of the most geologically and astrobiologically enticing materials in the Solar System. The area around Mawrth Vallis contains a vast (>1?×?10? km2) deposit of phyllosilicate-rich, ancient, layered rocks. A thick (>150?m) stratigraphic section that exhibits spectral evidence for nontronite, montmorillonite, amorphous silica, kaolinite, saponite, other smectite clay minerals, ferrous mica, and sulfate minerals indicates a rich geological history that may have included multiple aqueous environments. Because phyllosilicates are strong indicators of ancient aqueous activity, and the preservation potential of biosignatures within sedimentary clay deposits is high, martian phyllosilicate deposits are desirable astrobiological targets. The proposed MSL landing site at Mawrth Vallis is located directly on the largest and most phyllosilicate-rich deposit on Mars and is therefore an excellent place to explore for evidence of life or habitability.     

Computational aerodynamic analysis on perimeter reinforced （PR）-compliant wing

Abstract Implementing the morphing technique on a micro air vehicle （MAV） wing is a very chal- lenging task, due to the MAWs wing size limitation and the complex morphing mechanism. As a result, understanding aerodynamic characteristics and flow configurations, subject to wing structure deformation of a morphing wing MAV has remained obstructed. Thus, this paper presents the investigation of structural deformation, aerodynamics performance and flow formation on a pro- posed twist morphing MAV wing design named perimeter reinforced （PR）-compliant wing. The numerical simulation of two-way fluid structure interaction （FSI） investigation consist of a quasi- static aeroelastic structural analysis coupled with 3D incompressible Reynolds-averaged Navier- Stokes and shear-stress-transport （RANS-SST） solver utilized throughout this study. Verification of numerical method on a rigid rectangular wing achieves a good correlation with available exper- imental results. A comparative aeroelastic study between PR-compliant to PR and rigid wing per- formance is organized to elucidate the morphing wing performances. Structural deformation results show that PR-compliant wing is able to alter the wing＇s geometric twist characteristic, which has directly influenced both the overall aerodynamic performance and flow structure behavior. Despite the superior lift performance result, PR-compliant wing also suffers from massive drag penalty, which has consequently affected the wing efficiency in general. Based on vortices investigation, the results reveal the connection between these aerodynamic performances with vortices formation on PR-comoliant wing.     

The microbial habitability of weathered volcanic glass inferred from continuous sensing techniques

Basaltic glasses (hyaloclastite) are a widespread habitat for life in volcanic environments, yet their interior physical conditions are poorly characterized. We investigated the characteristics of exposed weathered basaltic glass from a surface outcrop in Iceland, using microprobes capable of continuous sensing, to determine whether the physical conditions in the rock interior are hospitable to microbial life. The material provided thermal protection from freeze-thaw and rapid temperature fluctuations, similar to data reported for other rock types. Water activity experiments showed that at moisture contents less than 13% wet weight, the glass and its weathering product, palagonite, had a water activity below levels suitable for bacterial growth. In pore spaces, however, these higher moisture conditions might be maintained for many days after a precipitation event. Gas exchange between the rock interior and exterior was rapid (< 10 min) when the rocks were dry, but when saturated with water, equilibration took many hours. During this period, we demonstrated the potential for low oxygen conditions within the rock caused by respiratory stimulation of the heterotrophic community within. These conditions might exist within subglacial environments during the formation of the rocks or in micro-environments in the interior of exposed rocks. The experiments showed that microbial communities at the site studied here could potentially be active for 39% of the year, if the depth of the community within the outcrop maintains a balance between access to liquid water and adequate protection from freezing. In the absence of precipitation, the interior of weathered basaltic glass is an extreme and life-limiting environment for microorganisms on Earth and other planets.     

液氧/液氢高压膨胀循环发动机初步试验

膨胀循环具有很好的性能和很高的可靠性,这一点已由普拉特·惠特尼公司的“半人马座”上面级推进系统RL10发动机证实,但是这种循环对燃烧室压力增加和推力增加都有限制作用,因为它仅仅利用了燃料在冷却燃烧室和喷管延伸段时吸收的热能,来推进燃料和氧化剂涡轮泵.建议采用具有内部热交换器的先进方案燃烧室,这种热交换器可以收集许多燃烧热量.叙述使用内部热交换器的高压膨胀循环发动机的主要性能,并给出了这种发动机的初步试验结果.热交换器中的平均热通量是Bartz相关计算值的70%.燃烧性能不会因装了内部热交换器而受到严重影响.     

Some ecological mechanisms to generate habitability in planetary subsurface areas by chemolithotrophic communities: the Río Tinto subsurface ecosystem as a model system

Chemolithotrophic communities that colonize subsurface habitats have great relevance for the astrobiological exploration of our Solar System. We hypothesize that the chemical and thermal stabilization of an environment through microbial activity could make a given planetary region habitable. The MARTE project ground-truth drilling campaigns that sampled cryptic subsurface microbial communities in the basement of the Río Tinto headwaters have shown that acidic surficial habitats are the result of the microbial oxidation of pyritic ores. The oxidation process is exothermic and releases heat under both aerobic and anaerobic conditions. These microbial communities can maintain the subsurface habitat temperature through storage heat if the subsurface temperature does not exceed their maximum growth temperature. In the acidic solutions of the Río Tinto, ferric iron acts as an effective buffer for controlling water pH. Under anaerobic conditions, ferric iron is the oxidant used by microbes to decompose pyrite through the production of sulfate, ferrous iron, and protons. The integration between the physical and chemical processes mediated by microorganisms with those driven by the local geology and hydrology have led us to hypothesize that thermal and chemical regulation mechanisms exist in this environment and that these homeostatic mechanisms could play an essential role in creating habitable areas for other types of microorganisms. Therefore, searching for the physicochemical expression of extinct and extant homeostatic mechanisms through physical and chemical anomalies in the Mars crust (i.e., local thermal gradient or high concentration of unusual products such as ferric sulfates precipitated out from acidic solutions produced by hypothetical microbial communities) could be a first step in the search for biological traces of a putative extant or extinct Mars biosphere.