The Dynamic Albedo of Neutrons (DAN) experiment for NASA's 2009 Mars Science Laboratory

We present a summary of the physical principles and design of the Dynamic Albedo of Neutrons (DAN) instrument onboard NASA's 2009 Mars Science Laboratory (MSL) mission. The DAN instrument will use the method of neutron-neutron activation analysis in a space application to study the abundance and depth distribution of water in the martian subsurface along the path of the MSL rover.     

Can identification of a fourth domain of life be made from sequence data alone, and could it be done on Mars?

A central question in astrobiology is whether life exists elsewhere in the universe. If so, is it related to Earth life? Technologies exist that enable identification of DNA- or RNA-based microbial life directly from environmental samples here on Earth. Such technologies could, in principle, be applied to the search for life elsewhere; indeed, efforts are underway to initiate such a search. However, surveying for nucleic acid-based life on other planets, if attempted, must be carried out with caution, owing to the risk of contamination by Earth-based life. Here we argue that the null hypothesis must be that any DNA discovered and sequenced from samples taken elsewhere in the universe are Earth-based contaminants. Experience from studies of low-biomass ancient DNA demonstrates that some results, by their very nature, will not enable complete rejection of the null hypothesis. In terms of eliminating contamination as an explanation of the data, there may be value in identification of sequences that lie outside the known diversity of the three domains of life. We therefore have examined whether a fourth domain could be readily identified from environmental DNA sequence data alone. We concluded that, even on Earth, this would be far from trivial, and we illustrate this point by way of examples drawn from the literature. Overall, our conclusions do not bode well for planned PCR-based surveys for life on Mars, and we argue that other independent biosignatures will be essential in corroborating any claims for the presence of life based on nucleic acid sequences.     

Biomarker and stable isotope characterization of coastal pond-derived organic matter, McMurdo Dry Valleys, Antarctica

Small coastal ponds that contain photosynthetic microbial mat communities represent an extreme environment where a potentially significant source of labile organic carbon can be found within the McMurdo Dry Valleys, Antarctica. To distinguish coastal pond-derived organic matter from other sources of organic matter in the Dry Valleys, bulk organic carbon, nitrogen, and sulfur isotope signatures and phospholipid fatty acid (PLFA) profiles of benthic microbial mats located at two sites--Hjorth Hill coast and Garwood Valley--were investigated. The average isotope values at Hjorth Hill coast and Garwood Valley are, respectively, -10.9 per thousand and -10.2 per thousand for delta(13) C, 3.7 per thousand and -1.3 per thousand for delta(15)N, and 8.1 per thousand and 16.7 per thousand for delta(34)S. Microbial mats from all ponds are dominated by monounsaturated PLFAs (indicative of Gram-negative bacteria) and polyunsaturated PLFAs (indicative of microeukaryotes). Biomarkers specific to aerobic prokaryotes, eukaryotes, and photoautotrophic microeukaryotes, as well as sulfur-reducing bacteria, are present in all samples. Benthic mats at Garwood Valley are thicker and more laminated, have a higher biomass, and have a greater carbon and nitrogen content, which suggests greater productivity than mats at Hjorth Hill coast. Greater productivity is supported, as well, by higher dissolved oxygen contents likely derived from heightened photosynthetic productivity. More productivity at Garwood Valley likely results from a larger influx of terrestrial surface waters together with a concomitant nutrient loading.     

Bacterial community in ancient Siberian permafrost as characterized by culture and culture-independent methods

The microbial composition of ancient permafrost sediments from the Kolyma lowland of Northeast Eurasia was examined through culture and culture-independent approaches. These sediments have been continuously frozen for 5,000 to 2-3 million years. A total of 265 Bacteria 16S rRNA gene sequences were amplified from the permafrost total-community genomic DNA and screened by amplified ribosomal 16S rRNA restriction analysis. Members of three major lineages were found: gamma-Proteobacteria (mostly Xanthomonadaceae), Actinobacteria, and Firmicutes. We also determined partial 16S rRNA gene sequences of 49 isolates from a collection of 462 aerobes isolated from these sediments. The bacteria included Actinomycetales (Arthrobacter and Microbacteriaceae); followed by the Firmicutes (Exiguobacterium and Planomicrobium); the Bacteroidetes (Flavobacterium); the gamma-Proteobacteria (Psychrobacter); and the alpha-Proteobacteria (Sphingomonas). Both culture and culture-independent approaches showed the presence of high and low G+C Gram-positive bacteria and gamma-Proteobacteria. Some of the 16S rRNA gene sequences of environmental clones matched those of Arthrobacter isolates. Two-thirds of the isolates grew at -2.5 degrees C, indicating that they are psychroactive, and all are closely related to phylogenetic groups with strains from other cold environments, mostly commonly from Antarctica. The culturable and non-culturable microorganisms found in the terrestrial permafrost provide a prototype for possible life on the cryogenic planets of the Solar System.     

Parametric models for helicopter identification using ANN

An artificial neural network (ANN) based helicopter identification system is proposed. The feature vectors are based on both the tonal and the broadband spectrum of the helicopter signal, ANN pattern classifiers are trained using various parametric spectral representation techniques. Specifically, linear prediction, reflection coefficients, cepstrum, and line spectral frequencies (LSF) are compared in terms of recognition accuracy and robustness against additive noise. Finally, an 8-helicopter ANN classifier is evaluated. It is also shown that the classifier performance is dramatically improved if it is trained using both clean data and data corrupted with additive noise.     

Radio plasma imager simulations and measurements

The Radio Plasma Imager (RPI) will be the first-of-its kind instrument designed to use radio wave sounding techniques to perform repetitive remote sensing measurements of electron number density (N _e) structures and the dynamics of the magnetosphere and plasmasphere. RPI will fly on the Imager for Magnetopause-to-Aurora Global Exploration (IMAGE) mission to be launched early in the year 2000. The design of the RPI is based on recent advances in radio transmitter and receiver design and modern digital processing techniques perfected for ground-based ionospheric sounding over the last two decades. Free-space electromagnetic waves transmitted by the RPI located in the low-density magnetospheric cavity will be reflected at distant plasma cutoffs. The location and characteristics of the plasma at those remote reflection points can then be derived from measurements of the echo amplitude, phase, delay time, frequency, polarization, Doppler shift, and echo direction. The 500 m tip-to-tip X and Y (spin plane) antennas and 20 m Z axis antenna on RPI will be used to measures echoes coming from distances of several R _E. RPI will operate at frequencies between 3 kHz to 3 MHz and will provide quantitative N _e values from 10^–1 to 10⁵ cm^–3. Ray tracing calculations, combined with specific radio imager instrument characteristics, enables simulations of RPI measurements. These simulations have been performed throughout an IMAGE orbit and under different model magnetospheric conditions. They dramatically show that radio sounding can be used quite successfully to measure a wealth of magnetospheric phenomena such as magnetopause boundary motions and plasmapause dynamics. The radio imaging technique will provide a truly exciting opportunity to study global magnetospheric dynamics in a way that was never before possible.     

Designing corrections for the trajectory of the <Emphasis Type="Italic">Spektr-R</Emphasis> spacecraft in the event of immersions into the Moon’s sphere of influence

The results of updating the parameters of motion of the Spektr-R spacecraft at the end of 2016 have shown that, in January 2018, with a probability close to unity, the condition that a spacecraft stay in the Earth’s shadow is violated; however, in May of the same year, the ballistic life of the spacecraft will be terminated. Thus, in 2017, the question arose of how to design the correction of flight of this spacecraft using its onboard propulsion system. The correction was designed with allowance for the fact that, for the first time since it was launched, the spacecraft in the course of several years, beginning with 2017, repeatedly approaches the Moon, deeply immersing into its sphere of influence. This paper presents the technologically and organizationally convenient, allowable versions of upcoming correction of the Spektr-R spacecraft trajectory and justifies the particular scheme of its implementation.     

Inflatable composite habitat structures for lunar and mars exploration

Recent advances in materials technology have improved the performance capabilities of inflatable, flexible composite structures, which have increased their potential for use in numerous space applications. Space suits, which are comprised of flexible composite components, are a good example of the successful use of inflatable composite structures in space. Space suits employ inflatables technology to provide a stand alone spacecraft for astronauts during extra-vehicular activity. A natural extension of this application of inflatables technology is in orbital or planetary habitat structures. NASA Johnson Space Center (JSC) is currently investigating flexible composite structures deployed via inflation for use as habitats, transfer vehicles and depots for continued exploration of the Moon and Mars.

Inflatable composite structures are being investigated because they offer significant benefits over conventional structures for aerospace applications. Inflatable structures are flexible and can be packaged in smaller and more complex shaped volumes, which result in the selection of smaller launch vehicles which dramatically reduce launch costs. Inflatable composite structures are typically manufactured from materials that have higher strength to weight ratios than conventional systems and are therefore lower in mass. Mass reductions are further realized because of the tailorability of inflatable composite structures, which allow the strength of the system to be concentrated where needed. Flexible composite structures also tend to be more damage tolerant due to their “forgiveness” as compared to rigid mechanical systems. In addition, inflatables have consistently proven to be lower in both development and manufacturing costs.

Several inflatable habitat development programs are discussed with their increasing maturation toward use on a flight mission. Selected development programs being discussed include several NASA Langley Research Center habitat programs that were conducted in the 1960s, the Lawrence Livermore National Laboratory inflatable space station study, the NASA JSC deployable inflatable Lunar habitat study, and the inflatable Mars TransHab study and test program currently ongoing at NASA JSC. Relevant technology developments made by ILC Dover are also presented.     

9. The venus ionosphere and solar wind interaction

The current state of knowledge of the chemistry, dynamics and energetics of the upper atmosphere and ionosphere of Venus is reviewed together with the nature of the solar wind-Venus interaction. Because of the weak, though perhaps not negligible, intrinsic magnetic field of Venus, the mutual effects between these regions are probably strong and unique in the solar system. The ability of the Pioneer Venus Bus and Orbiter experiments to provide the required data to answer the questions outstanding is discussed in detail.