Terahertz circular dichroism spectroscopy: a potential approach to the in situ detection of life's metabolic and genetic machinery

We propose a terahertz (far-infrared) circular dichroism-based life-detection technology that may provide a universal and unequivocal spectroscopic signature of living systems regardless of their genesis. We argue that, irrespective of the specifics of their chemistry, all life forms will employ well-structured, chiral, stereochemically pure macromolecules (>500 atoms) as the catalysts with which they perform their metabolic and replicative functions. We also argue that nearly all such macromolecules will absorb strongly at terahertz frequencies and exhibit significant circular dichroism, and that this circular dichroism unambiguously distinguishes biological from abiological materials. Lastly, we describe several approaches to the fabrication of a terahertz circular dichroism spectrometer and provide preliminary experimental indications of their feasibility. Because terahertz circular dichroism signals arise from the molecular machinery necessary to carry out life's metabolic and genetic processes, this life-detection method differs fundamentally from more well-established approaches based on the detection of isotopic fractionation, "signature" carbon compounds, disequilibria, or other by-products of metabolism. Moreover, terahertz circular dichroism spectroscopy detects this machinery in a manner that makes few, if any, assumptions as to its chemical nature or the processes that it performs.     

Deep Impact: A Large-Scale Active Experiment on a Cometary Nucleus

The Deep Impact mission will provide the first data on the interior of a cometary nucleus and a comparison of those data with data on the surface. Two spacecraft, an impactor and a flyby spacecraft, will arrive at comet 9P/Tempel 1 on 4 July 2005 to create and observe the formation and final properties of a large crater that is predicted to be approximately 30-m deep with the dimensions of a football stadium. The flyby and impactor instruments will yield images and near infrared spectra (1–5 μm) of the surface at unprecedented spatial resolutions both before and after the impact of a 350-kg spacecraft at 10.2 km/s. These data will provide unique information on the structure of the nucleus near the surface and its chemical composition. They will also used to interpret the evolutionary effects on remote sensing data and will indicate how those data can be used to better constrain conditions in the early solar system.     

United States space policy and international partnership

Has the current US space policy improved the USA's overall strategic position? Does it affect favorably international partnerships? These questions are examined in terms of security, political economy, and influence. In today's context, where there are more space players, more options, more potential for unintended consequences, and higher stakes, unilateral action is more limited in its effectiveness than in earlier times. Surveying current US space policy, it is not clear that data-driven, analytically based decisions are being made to affect positively national independence, innovation, market creation, and international perceptions of the USA as a trustworthy partner. More promising are the steps taken to bolster a predictable space operational environment and economic competitiveness. Ultimately, in order to achieve American excellence and leadership, a ‘closed loop’ on the policy system is needed, to gauge regularly and systematically whether the US is achieving the desired national outcomes.     

Swords into ploughshares Missiles as commercial launchers

The signing of an INF arms control treaty in 1987 and the reduction in East-West tension has resulted in a surplus of missiles. Getting rid of them remains a problem, but two distinct initiatives have been advanced in the USA and the USSR for their conversion into low-cost launchers. The author describes these two initiatives and discusses the political and economic issues they raise before speculating on the likelihood of their achievement.     

The origin and early evolution of membrane channels

The origin and early evolution of ion channels are considered from the point of view that the transmembrane segments of membrane proteins are structurally quite simple and do not require specific sequences to fold. We argue that the transport of solute species, especially ions, required an early evolution of efficient transport mechanisms, and that the emergence of simple ion channels was protobiologically plausible. We also argue that, despite their simple structure, such channels could possess properties that, at the first sight, appear to require markedly greater complexity. These properties can be subtly modulated by local modifications to the sequence rather than global changes in molecular architecture. In order to address the evolution and development of ion channels, we focus on identifying those protein domains that are commonly associated with ion channel proteins and are conserved throughout the three main domains of life (Eukarya, Bacteria, and Archaea). We discuss the potassium-sodium-calcium superfamily of voltage-gated ion channels, mechanosensitive channels, porins, and ABC-transporters and argue that these families of membrane channels have sufficiently universal architectures that they can readily adapt to the diverse functional demands arising during evolution.     

Microbial diversity of Indian Ocean hydrothermal vent plumes: microbes tolerant of desiccation, peroxide exposure, and ultraviolet and gamma-irradiation

The microbial diversity of Kali chimney plumes, part of a hydrothermal vent field in the Rodriguez Triple Junction, Indian Ocean (depth approximately 2,240 m), was examined in an attempt to discover "extremotolerant" microorganisms that have evolved unique resistance capabilities to this harsh environment. Water and sediment samples were collected from the vent and from sediments located at various distances (2-20 m) away from and surrounding the chimney. Samples were screened for hypertolerant microbes that are able to withstand multiple stresses. A total of 46 isolates were selected for exposure to a number of perturbations, such as heat shock, desiccation, H(2)O(2), and ultraviolet (UV) and gamma-irradiation. The survival of Psychrobacter sp. L0S3S-03b following exposure to >1,000 J/m(2) UV(254) radiation was particularly intriguing amid a background of varying levels of resistance. Vegetative cells of this non-spore-forming microbe not only survived all of the treatments, but also exhibited a 90% lethal dose of 30 s when exposed to simulated martian UV radiation and a 100% lethal dose of 2 min when exposed to full spectrum UV, which is comparable to findings for bacterial endospores.