Identification of specific gravity sensitive signal transduction pathways in human A431 carcinoma cells.

Epidermal growth factor (EGF) activates a well characterized signal transduction cascade in human A431 epidermoid carcinoma cells. The influence of gravity on EGF-induced EGF-receptor clustering and early gene expression as well as on actin polymerization and actin organization have been investigated. Different signalling pathways induced by the agents TPA, forskolin and A23187 that activate gene expression were tested for sensitivity to gravity. EGF-induced c-fos and c-jun expression were decreased in microgravity. However, constitutive beta-2 microglobulin expression remained unaltered. Under simulated weightlessness conditions EGF- and TPA-induced c-fos expression was decreased, while forskolin- and A23187-induced c-fos expression was independent of the gravity conditions. These results suggest that gravity affects specific signalling pathways. Preliminary results indicate the EGF-induced EGF-receptor clustering remained unaltered irrespective of the gravity conditions. Furthermore, the relative filamentous actin content of steady state A431 cells was enhanced under microgravity conditions and actin filament organization was altered. Under simulated weightlessness actin filament organization in steady state cells as well as in EGF-treated cells was altered as compared to the 1 G reference experiment. Interestingly the microtubule and keratin organization in untreated cells showed no difference with the normal gravity samples. This indicates that gravity may affect specific components of the signal transduction circuitry.     

Aircraft vertical profile implementation using directed-graphmethods

Aircraft vertical profile simulation is realized using a demand-driven minimal-calculation directed graph structure to reduce calculation time and to force synchronization of the performance measurement functions with the system state variables. Performance-directed model adaptation makes dynamic vertical profile path corrections, in the presence of fixed drag variations, possible. Drag variations ranging from +10% to -10% yielded fuel consumption improvements of less than 1% in the majority of the cases. Calculation time improvement for path simulation ranges from a factor of 1.19 in the worst case to 1.5 in the best case     

Earth benefits from space life sciences

Contributions of space exploration which are widely recognized are those dealing with the impact of space technology on public health and medical services in both urban and remote rural areas. Telecommunications, image enhancement, 3-dimensional image reconstructions, miniaturization, automation, and data analysis, have transformed the delivery of medical care and have brought about a new impetus to the field of biomedicine. Many areas of medical care and biological research have been affected. These include technological breakthroughs in such areas as: (1) diagnosis, treatment, and prevention of cardiovascular diseases, (2) new approaches to the understanding of osteoporosis, (3) early detection of genetic birth defects, (4) emergency medical care, and (5) treatment of chronic metabolic disorders. These are but a few examples where technology originally developed to support space medicine or space research has been applied to solving medical and health care delivery problems on Earth.     

IR-spectroscopy of ionosphere from stratospheric balloons

The radiation spectra of the ionosphere in the 4–5 μm region have been determined from stratospheric balloons by means of the specially elaborated method of the registration of angular and spectral distribution of the radiation. The radiation bands of 4.4 μm and 4.8 μm which have 0.1–0.2 erg cm^?2 sr^?1s^?1. brightness obtained in two flights, are identified with the vibration-rotation transitions of the ion NO⁺ (0 - 1) and the molecules N¹⁴N¹⁵ (0 - 1) and CO (0 - 1) and (3 - 2) from altitudes between 120 and 500 km.     

Performance Degradation of MTI Circuits Due to Amplitude Limiting

The loss in output signal-to-noise ratio (SNR) due to amplitude limiting is obtained for a radar circuit consisting of a bandpass limiter, coherent demodulator, matched filter, and moving-target-indicator (MTI) filter. The circuit is used in scanning MTI radars. The tandem connection of the limiter and coherent demodulator is a model for the saturation of the intermediate-frequency (IF) demodulator of an MTI radar. Results on special functions are used to obtain simple formulas for the loss in output SNR relative to a linear IF demodulator when the input SNR is less than -15 dB and the number of hits per 3-dB beamwidth exceeds 15.     

Vertical profiles of halocarbons in the stratosphere

Stratospheric air samples collected between 10 and 35 km altitude my means of a cryogenic sampler were analyzed by gaschromatography. Thus vertical profiles of source gases for halogen radicals were derived, such as CCl₄, CCl₃F, CCl₂F₂, CClF₃, CF₄, C₂F₃Cl₃, C₂F₄Cl₂, C₂F₅Cl, C₂F₆, CH₃Cl, CH₃CCl₃, CHF₂Cl, CH₃Br, CBrF₃, and CBrCl₂F. Systematic discrepancies between measured and modelled halocarbon profiles point to deficiencies of present one- and two-dimensional models. Measurements of fully halogenated hydrocarbons provide a tool for systematically studying these deficiencies and thus improving the models.     

ELF radio signals produced in the auroral ionosphere by non-linear demodulation of signals from high-power amplitude-modulated transmitters

ELF and VLF radio signals were recorded in the afternoon to early morning (local time) between 24 March and 4 April 1979, in Northern Scandinavia. Apart from signals of natural origin, timing signals, i.e. six pips of equal duration of 105 ± 8 ms, at 1 kHz ± 0.5 Hz, were observed on the hour UT. Such signals only occur on days of relatively high geomagnetic activity during enhanced auroral electrojet activity. They are believed to be generated by non-linear demodulation (self-detection) of signals from two or more amplitude modulated transmitters in the USSR, operating at 173, 200, 236, 263 and 657 kHz. The simplest explanation for the observations is provided by the three transmitters operating at 173 kHz.     

Metallic multilayers: New possibilities in X-UV optics

Reflectivities measured on metallic multilayers obtained by evaporation methods have demonstrated the validity of theoretical predictions. Experimental tests using wavelengths from 1.54 Å to several hundred of angstroems give a firm basis for proposed new designs for X-UV optics. This new kind of Bragg reflector can be tailored for any wavelength and any incidence angle, because the period of evaporation of the layered synthetic medium can be freely chosen. Such artificial Bragg reflectors are more efficient than natural, organic or molecular crystals which have been employed until now in the X-UV, and can overcome the weak luminosity and astigmatism problems encountered in grazing incidence optics. The possibility of evaporating multilayers on shaped substrates will lead to optics with focussing and selectivity fuctions given by a single reflection.     

Simulation of Permanent Magnet Generator/Rectifier Combination

Permanent magnet generators are often used as the pilot exciters of three-stage generating units to provide an efficient and reliable source of electrical power. No external power supply is then necessary, and the problems associated with brushwear and with arcing at the rubbing contacts are eliminated. A technique is presented here by which the performance of a permanent magnet generator, when combined with a rectifier-fed d.c. load at its output terminals, may be accurately and efficiently computed. The machine model takes into account the magnetization characteristic of the permanent magnet rotor and involves only parameters which can be determined from terminal measurements on the machine at standstill. Both computed and measured characteristics for an experimental machine are presented, and a comparison of these characteristics shows that the model can predict accurately all the major characteristics of the machine, as well as its detailed internal performance, when supplying a typical resistiveinductive load.     

A Nonlinear Tracker Using Attitude Measurements

The subject of this paper involves tracking the present position of a maneuvering aircraft as well as predicting its future position. A tracking filter is developed that uses aircraft attitude angles (yaw, pitch, roll) in addition to the usual radar measurements. Computer simulation of tracker performance when tracking violently maneuvering aircraft indicates that a dramatic improvement is obtained by using attitude information. The approach taken is to develop a 12-or 15-state extended Kalman filter that models both translational and rotational degrees of freedom. By measuring and estimating attitude it is possible to approximately determine the magnitude and direction of the force system acting on the vehicle and therefore determine vehicle linear acceleration. Knowledge of acceleration is then used to improve the estimate of present and future position of the vehicle being tracked. Simulation of a T-38 aircraft performing a 5 g turn indicates that the new tracker produces maximum trajectory prediction errors that are 36 percent of the errors experienced by more conventional trackers.