Plant cell plasma membrane structure and properties under clinostatting.

Structural-functional organization of plasma membrane of pea roots seedling was investigated by methods of chemiluminescence, fluorescence probes, chromatography and freeze-fracture studies under normal conditions and clinostatting. Phase character of lipid peroxidation intensity was fixed. The initial phase of this process is characterized by lipid peroxidation decreasing with its next induction. The primary changes depending on free-radical mechanisms of lipid peroxidation were excellently revealed by chemiluminescence. Plasmalemma microviscosity increased on the average of 15-20% under microgravity at the initial stages of its phenomenon. There were major changes of phosphatidilcholine and phosphatidilethanolamine contents. The total quantity of phospholipids remained rather stable. Changes of phosphatide acid concentration point to degradation and phospholipids biosynthesis. There were increases of unsaturated fatty acids mainly at the expense of linoleic and linolenic acids and also a decrease of saturated fatty acid content at the expense of palmitic and stearic acids. Unsaturation index of fatty acids increased as well. On the whole fatty acid composition was variable in comparison with phospholipids. Probably it is one of mechanisms of maintaining of microviscosity within definite limits. Considerable structural changes in organization of plasmalemma protein-lipid complex were not revealed by the freeze-fracture studies.     

Long-term preservation of microbial ecosystems in permafrost.

It has been established that significant numbers (up to 10 million cells per gram of sample) of living microorganisms of various ecological and morphological groups have been preserved under permafrost conditions, at temperatures ranging from -9 to -13 degrees C and depths of up to 100 m, for thousands and sometimes millions of years. Preserved since the formation of permafrost in sand-clay sediments of the Pliocene-Quaternary period and in paleosols and peats buried among them, these cells art the only living organisms that have survived for a geologically significant period of time. The complexity of the microbial community preserved varies with the age of the permafrost. Eukaryotes are found only in Holocene sediments; while prokaryotes are found to greater ages, i.e., Pliocene and Pleistocene. The diversity of microorganisms decreases with increasing age of sediments, and as a result cocci and corynebacteria are predominant. Enzyme activity (catalase and hydrolytic enzymes) and photosynthetic pigments (chlorophyll and pheophytin have also been detected in permafrost sediments. These results permit us to outline some approaches to the search for traces of life in the permafrost of Martian sediments by borehole core sampling. It is in the deep horizons (and not on the planet surface), isolated by permafrost from the external conditions, that results similar to those obtained on Earth can be expected.     

Sikkim's Teesta River fog, mist and dust particle monitoring using monostatic LIDAR

Fog, mist, and atmospheric dust particles, having the dimension of one micrometer or less, play an important role in the deterioration of visibility, as well as in causing local warming in the atmosphere. With an attempt to reduce the deterioration, a scientific approach has to be taken to determine their origins. A monostatic LIDAR may be one of the best instruments for such work. The authors are tempted to develop such a LIDAR for fog, mist, and dust particle monitoring over River Teesta at Sikkim. LIDAR is an acronym for Light Detection And Ranging. What can we do with LIDAR? Measure distance, measure speed, measure rotation, measure chemical composition and concentration, and measure cross-sections of the targets. The digital technique is always utilized for its development which results in better security, lower power consumption, higher power efficiency, higher reliability, lower transmitter power, lower multipath effect, higher interference suppression as compared to an analog system. The commercial systems like disdometer, rain radar, mobile robot, etc., utilizing LIDAR principles are operational in different parts of the world. The authors are highly motivated for such LIDAR development and their development effort follows.     

Dynamic trapping of electrons in the porcupine ionospheric ion beam experiment

Electrons are needed to maintain quasineutrality in a case where positive ions are injected across the magnetic field into a limited volume in a magnetized plasma. In the absence of collisions, a positive potential builds up and traps the electrons which enter the region along the magnetic field. If the added density of ions exceeds the ambient density, large potential differences along the magnetic field can be maintained this way. The process explains several features of the Porcupine xenon ion beam injection experiment, where strong magnetic-field-aligned electric fields were measured in the vicinity of a xenon ion beam which was injected into the ambient ionosphere from a spinning subpayload.     

Error Covariance Analysis of a Karhunen-Loeve Collocation Estimator

An error covariance analysis of a two-dimensional gravity compensation technique (KLC) employing a Karhunen-Loeve gravity disturbance model and the linear least-square collocation algorithm for its estimation is presented, without actually using any data. Its performance is compared with another gravity compensation technique (KLE), whose error covariance analysis was previously presented by Gupta. From the mismodeling analysis, KLC appears to be superior to KLE.     

Observation of enhanced sub-iron (Sc---Cr) to iron ration in low energy cosmic rays of 50–100 MeV/N in spacelab-3

Relative abundances of sub-iron (Sc-Cr) to iron nuclei in low energy (50–100 MeV/N) galactic cosmic rays have been determined from an analysis of about 100 events of heavy ions (Z = 10−28) recorded in a detector assembly flown in the Anuradha cosmic ray experiment in the Spacelab-3 on a six day mission in April–May 1985. The measured abundance ratio of (Sc-Cr)/Fe nuclei in 50–100 MeV/N energy range is 1.1 ± 0.3, and the present result of enhanced ratio of sub-iron to iron nuclei is in agreement with other experimental results in 200–800 MeV/N range. The over-abundance of iron secondaries at these low energies cannot be explained in the conventional models for propagation of cosmic rays. Available experimental data indicate a very different time history for the low energy iron-group, as compared to those of lighter nuclei in galactic cosmic rays.     

Mammalian cell cultivation in space.

Equipment used in space for the cultivation of mammalian cells does not meet the usual standard of earth bound bioreactors. Thus, the development of a space worthy bioreactor is mandatory for two reasons: First, to investigate the effect on single cells of the space environment in general and microgravity conditions in particular, and second, to provide researchers on long term missions and the Space Station with cell material. However, expertise for this venture is not at hand. A small and simple device for animal cell culture experiments aboard Spacelab (Dynamic Cell Culture System; DCCS) was developed. It provides 2 cell culture chambers, one is operated as a batch system, the other one as a perfusion system. The cell chambers have a volume of 200 microliters. Medium exchange is achieved with an automatic osmotic pump. The system is neither mechanically stirred nor equipped with sensors. Oxygen for cell growth is provided by a gas chamber that is adjacent to the cell chambers. The oxygen gradient produced by the growing cells serves to maintain the oxygen influx by diffusion. Hamster kidney cells growing on microcarriers were used to test the biological performance of the DCCS. On ground tests suggest that this system is feasible.     

Ultrastructural and growth indices of Chlorella culture in multicomponent aquatic systems under space flight conditions.

Submicroscopic organization of Chlorella cells cultivated under space flight conditions in three-component aquatic system has been studied. Comparison of the experimental cells with that of the controls revealed certain rearrangements of cell organelles particularly, a reduction in the amount of reserve polysaccharides in chloroplasts, increase of cell vacuolization and mitochondrion volume, complication configuration of plasmalemma evaginations and invaginations, and also disturbances in the process of cytokinesis. More over an increase in the number of Chlorella cells infected by bacteria was shown in the experimental variant. No considerable differences were established in the growth characteristics of the experimental and control populations. A comparative cytological analysis revealed general regularities of organelles in Chlorella cells cultivated under space flight condition in the uni- and multicomponent systems.     

High LET-induced H2AX phosphorylation around the Bragg curve.

We investigated the spatial distribution of the induction of the phosphorylated form of the histone protein H2AX (gamma-H2AX), known to be activated by DSBs. Following irradiation of human fibroblast cells with 600 MeV/nucleon silicon and 600 MeV/nucleon iron ions we observed the formation of gamma-H2AX aggregates in the shape of streaks stretching over several micrometers in an x/y plane. Polyethylene shielding was used to achieve a Bragg curve distribution with beam geometry parallel to the monolayer of cells. We present data that highlights the formation of immunofluorescent gamma-H2AX tracks showing the ion trajectories across the Bragg peak of irradiated human fibroblast cells. Qualitative analyses of these distributions indicated potentially increased clustering of DNA damage before the Bragg peak, enhanced gamma-H2AX distribution at the peak, and provided visual evidence of high-linear energy transfer particle traversal of cells beyond the Bragg peak in agreement with one-dimensional transport approximations. Spatial assessment of gamma-H2AX fluorescence may provide direct insights into DNA damage across the Bragg curve for high charge and energy ions including the biological consequences of shielding and possible contributors to bystander effects.