Damage escape and repair in dried Chroococcidiopsis spp. from hot and cold deserts exposed to simulated space and martian conditions

The cyanobacterium Chroococcidiopsis, overlain by 3?mm of Antarctic sandstone, was exposed as dried multilayers to simulated space and martian conditions. Ground-based experiments were conducted in the context of Lichens and Fungi Experiments (EXPOSE-E mission, European Space Agency), which were performed to evaluate, after 1.5 years on the International Space Station, the survival of cyanobacteria (Chroococcidiopsis), lichens, and fungi colonized on Antarctic rock. The survival potential and the role played by protection and repair mechanisms in the response of dried Chroococcidiopsis cells to ground-based experiments were both investigated. Different methods were employed, including evaluation of the colony-forming ability, single-cell analysis of subcellular integrities based on membrane integrity molecular and redox probes, evaluation of the photosynthetic pigment autofluorescence, and assessment of the genomic DNA integrity with a PCR-based assay. Desiccation survivors of strain CCMEE 123 (coastal desert, Chile) were better suited than CCMEE 134 (Beacon Valley, Antarctica) to withstand cellular damage imposed by simulated space and martian conditions. Exposed dried cells of strain CCMEE 123 formed colonies, maintained subcellular integrities, and, depending on the exposure conditions, also escaped DNA damage or repaired the induced damage upon rewetting.     

Correction to: Water in the Earth’s Interior: Distribution and Origin

Space Science Reviews - Correction to: Space Sci Rev DOI This article has been corrected. Figure 3 was initially published with erroneous axis titles in Fig. 3B and 3D where the $x$ axis should be...     

Gravity Recovery and Interior Laboratory (GRAIL): Mapping the Lunar Interior from Crust to Core

The Gravity Recovery and Interior Laboratory (GRAIL) is a spacecraft-to-spacecraft tracking mission that was developed to map the structure of the lunar interior by producing a detailed map of the gravity field. The resulting model of the interior will be used to address outstanding questions regarding the Moon’s thermal evolution, and will be applicable more generally to the evolution of all terrestrial planets. Each GRAIL orbiter contains a Lunar Gravity Ranging System instrument that conducts dual-one-way ranging measurements to measure precisely the relative motion between them, which in turn are used to develop the lunar gravity field map. Each orbiter also carries an Education/Public Outreach payload, Moon Knowledge Acquired by Middle-School Students (MoonKAM), in which middle school students target images of the Moon for subsequent classroom analysis. Subsequent to a successful launch on September 10, 2011, the twin GRAIL orbiters embarked on independent trajectories on a 3.5-month-long cruise to the Moon via the EL-1 Lagrange point. The spacecraft were inserted into polar orbits on December 31, 2011 and January 1, 2012. After a succession of 19 maneuvers the two orbiters settled into precision formation to begin science operations in March 1, 2012 with an average altitude of 55 km. The Primary Mission, which consisted of three 27.3-day mapping cycles, was successfully completed in June 2012. The extended mission will permit a second three-month mapping phase at an average altitude of 23 km. This paper provides an overview of the mission: science objectives and measurements, spacecraft and instruments, mission development and design, and data flow and data products.     

Scientific visualization to study Flux Transfer Events at the Community Coordinated Modeling Center

In this paper we present recent additions to the visualization toolset offered by the Community Coordinated Modeling Center (CCMC). Two suites of visualization tools are available that can address different needs during the analysis of model simulations of the magnetosphere that are provided by the CCMC. The online, server-side visualization allows the user to quickly browse through simulation runs and now can create maps of magnetic field line topology in the magnetosphere. The second tool, SWX, can be used on the client computer after data have been downloaded. With this second tool the user can interact directly with the three-dimensional objects that are being rendered. We present results from a simulation of a Flux Transfer Event that was performed at the CCMC using a magnetohydrodynamic model of the Earth’s magnetosphere with a high resolution grid focused on the dayside magnetosheath and dayside magnetopause. The simulation shows that the FTE that results from localized magnetic reconnection is a complicated three-dimensional structure that requires modern visualization techniques. Visualization techniques that are presented here allow the researcher to fully appreciate the complexity contained in magnetospheric simulation results.     

On the practical exploitation of perturbative effects in low Earth orbit for space debris mitigation

This paper presents the results of a numerical evaluation of the natural lifetime reduction in low Earth orbit, due to dynamical perturbations. The study considers two values for the area-to-mass ratio, a nominal ratio which resembles a typical value of spacecraft in orbit today, and an enhanced ratio which covers the surface augmentation. The results were obtained with two orbit propagators, one of a semi-analytical nature and the second one using non-averaged equations of motion. The simulations for both propagators were set up similarly to allow comparison. They both use the solar radiation pressure and the secular terms of the geopotential ($J_2,J_4$ and $J_6$). The atmospheric drag was turned on and off in both propagators to alternatively study the eccentricity build up and the residual lifetime. The non-averaging case also covers a validation with the full 6?×?6 geopotential. The results confirm the findings in previous publications, that is, the possibility for de-orbiting from altitudes above the residual atmosphere if a solar sail is deployed at the end-of-life, due to the combined effect of solar radiation pressure and the oblateness of the Earth. At near polar inclinations, shadowing effects can be exploited to the same end. The results obtained with the full, non-averaging propagator revealed additional de-orbiting corridors associated with solar radiation pressure which were not found by previous work on space debris mitigation. The results of both tools are compared for specific initial conditions. For nominal values of area-to-mass ratio, instead, it is confirmed that this resonance effect is negligible.The paper then puts the findings in the perspective of the current satellite catalogue. It identifies space missions which are currently close to a resonance corridor and shows the orbit evolution within the resonances with a significantly shorter residual orbital lifetime. The paper finishes with a discussion on the exploitation of these effects with regards to the long-term simulation of the space debris environment and a flux and collision probability comparison.     

JIRAM, the image spectrometer in the near infrared on board the Juno mission to Jupiter

The Jovian InfraRed Auroral Mapper (JIRAM) has been accepted by NASA for inclusion in the New Frontiers mission "Juno," which will launch in August 2011. JIRAM will explore the dynamics and the chemistry of Jupiter's auroral regions by high-contrast imaging and spectroscopy. It will also analyze jovian hot spots to determine their vertical structure and infer possible mechanisms for their formation. JIRAM will sound the jovian meteorological layer to map moist convection and determine water abundance and other constituents at depths that correspond to several bars pressure. JIRAM is equipped with a single telescope that accommodates both an infrared camera and a spectrometer to facilitate a large observational flexibility in obtaining simultaneous images in the L and M bands with the spectral radiance over the central zone of the images. Moreover, JIRAM will be able to perform spectral imaging of the planet in the 2.0-5.0 microm interval of wavelengths with a spectral resolution better than 10 nm. Instrument design, modes, and observation strategy will be optimized for operations onboard a spinning satellite in polar orbit around Jupiter. The JIRAM heritage comes from Italian-made, visual-infrared imaging spectrometers dedicated to planetary exploration, such as VIMS-V on Cassini, VIRTIS on Rosetta and Venus Express, and VIR-MS on the Dawn mission.     

Severe disruption of the cytoskeleton and immunologically relevant surface molecules in a human macrophageal cell line in microgravity—Results of an in vitro experiment on board of the Shenzhou-8 space mission

During spaceflight the immune system is one of the most affected systems of the human body. During the SIMBOX (Science in Microgravity Box) mission on Shenzhou-8, we investigated microgravity-associated long-term alterations in macrophageal cells, the most important effector cells of the immune system. We analyzed the effect of long-term microgravity on the cytoskeleton and immunologically relevant surface molecules. Human U937 cells were differentiated into a macrophageal phenotype and exposed to microgravity or 1g on a reference centrifuge on-orbit for 5 days. After on-orbit fixation, the samples were analyzed with immunocytochemical staining and confocal microscopy after landing. The unmanned Shenzhou-8 spacecraft was launched on board a Long March 2F (CZ-2F) rocket from the Jiuquan Satellite Launch Center (JSLC) and landed after a 17-day-mission. We found a severely disturbed actin cytoskeleton, disorganized tubulin and distinctly reduced expression of CD18, CD36 and MHC-II after the 5 days in microgravity. The disturbed cytoskeleton, the loss of surface receptors for bacteria recognition, the activation of T lymphocytes, the loss of an important scavenger receptor and of antigen-presenting molecules could represent a dysfunctional macrophage phenotype. This phenotype in microgravity would be not capable of migrating or recognizing and attacking pathogens, and it would no longer activate the specific immune system, which could be investigated in functional assays. Obviously, the results have to be interpreted with caution as the model system has some limitations and due to numerous technical and biological restrictions (e.g. 23 °C and no CO₂ supply during in-flight incubation). All parameter were carefully pre-tested on ground. Therefore, the experiment could be adapted to the experimental conditions available on Shenzhou-8.     

Heuristics of node selection criteria to assess robustness of world airport network

The world airport network (WAN) is one of the networked infrastructures that shape today's economic and social activity,so its resilience against incidents affecting the WAN is an important problem.In this paper,the robustness of air route networks is extended by defining and testing several heuristics to define selection criteria to detect the critical nodes of the WAN.In addition to heuristics based on genetic algorithms and simulated annealing,custom heuristics based on node damage and node betweenness are defined.The most effective heuristic is a multiattack heuristic combining both custom heuristics.Results obtained are of importance not only for advance in the understanding of the structure of complex networks,but also for critical node detection.