SOLID3: a multiplex antibody microarray-based optical sensor instrument for in situ life detection in planetary exploration

The search for unequivocal signs of life on other planetary bodies is one of the major challenges for astrobiology. The failure to detect organic molecules on the surface of Mars by measuring volatile compounds after sample heating, together with the new knowledge of martian soil chemistry, has prompted the astrobiological community to develop new methods and technologies. Based on protein microarray technology, we have designed and built a series of instruments called SOLID (for "Signs Of LIfe Detector") for automatic in situ detection and identification of substances or analytes from liquid and solid samples (soil, sediments, or powder). Here, we present the SOLID3 instrument, which is able to perform both sandwich and competitive immunoassays and consists of two separate functional units: a Sample Preparation Unit (SPU) for 10 different extractions by ultrasonication and a Sample Analysis Unit (SAU) for fluorescent immunoassays. The SAU consists of five different flow cells, with an antibody microarray in each one (2000 spots). It is also equipped with an exclusive optical package and a charge-coupled device (CCD) for fluorescent detection. We demonstrated the performance of SOLID3 in the detection of a broad range of molecular-sized compounds, which range from peptides and proteins to whole cells and spores, with sensitivities at 1-2?ppb (ng?mL?1) for biomolecules and 10? to 103 spores per milliliter. We report its application in the detection of acidophilic microorganisms in the Río Tinto Mars analogue and report the absence of substantial negative effects on the immunoassay in the presence of 50?mM perchlorate (20 times higher than that found at the Phoenix landing site). Our SOLID instrument concept is an excellent option with which to detect biomolecules because it avoids the high-temperature treatments that may destroy organic matter in the presence of martian oxidants.     

A two-tiered approach to assessing the habitability of exoplanets

In the next few years, the number of catalogued exoplanets will be counted in the thousands. This will vastly expand the number of potentially habitable worlds and lead to a systematic assessment of their astrobiological potential. Here, we suggest a two-tiered classification scheme of exoplanet habitability. The first tier consists of an Earth Similarity Index (ESI), which allows worlds to be screened with regard to their similarity to Earth, the only known inhabited planet at this time. The ESI is based on data available or potentially available for most exoplanets such as mass, radius, and temperature. For the second tier of the classification scheme we propose a Planetary Habitability Index (PHI) based on the presence of a stable substrate, available energy, appropriate chemistry, and the potential for holding a liquid solvent. The PHI has been designed to minimize the biased search for life as we know it and to take into account life that might exist under more exotic conditions. As such, the PHI requires more detailed knowledge than is available for any exoplanet at this time. However, future missions such as the Terrestrial Planet Finder will collect this information and advance the PHI. Both indices are formulated in a way that enables their values to be updated as technology and our knowledge about habitable planets, moons, and life advances. Applying the proposed metrics to bodies within our Solar System for comparison reveals two planets in the Gliese 581 system, GJ 581 c and d, with an ESI comparable to that of Mars and a PHI between that of Europa and Enceladus.     

试论高职教育教材建设的基本思路

教材是实现教育目的的主要载体 ,教材编写得如何 ,更是能否体现高职教育特色的关键。本文针对教材建设 ,从教材编写的基本原则、基本要求及教材的发展趋势等方面提出了一些建议和设想     

Kinematics of Saturn's spokes

Voyager 2 images of Saturn's rings have been analyzed for spoke activity. More than 80 and 40 different spokes have been measured at the morning and at the evening ansa, respectively. Higher rate of spoke formation has been found at 145° ± 15° SLS and at 305° ± 15° SLS which persisted for at least 3 Saturn revolutions. Higher spoke activity (formation and growth in width) by more than a factor 3 has been observed over the nightside hemisphere of Saturn than over the dayside hemisphere. The age distribution (i.e. time from radial formation until observation, assuming Keplerian shear) of the leading (old) edges of spokes has its maximum at ～ 9,000 s and ～ 6,000 s for spokes observed at the morning ansa and at the evening ansa, respectively. The highest spoke age observed is ～ 20,000 s. The age distribution of the trailing (young) edges of spokes peaks at < 2,000 s at both ansae but has its mean at ～ 4,500 s and ～ 3,500 s, respectively. On the average the observed spokes grew in width for ～ 4,500 s at the morning ansa and for ～ 2,500 s at the evening ansa. The maximum time of growth in width was ～ 12,000 s.     

Remote sounding of atmospheric gravity waves with satellite limb and nadir techniques

Recent advances in satellite techniques hold great potential for mapping global gravity wave (GW) processes at various altitudes. Poor understanding of small-scale GWs has been a major limitation to numerical climate and weather models for making reliable forecasts. Observations of short-scale features have important implication for validating and improving future high-resolution numerical models. This paper summarizes recent GW observations and sensitivities from several satellite instruments, including MLS, AMSU-A, AIRS, GPS, and CLAES. It is shown in an example that mountain waves with horizontal wavelengths as short as 30 km now can be observed by AIRS, reflecting the superior horizontal resolution in these modern satellite instruments. Our studies show that MLS, AMSU-A and AIRS observations reveal similar GW characteristics, with the observed variances correlated well with background winds. As a complementary technique, limb sounding instruments like CRISTA, CLAES, and GPS can detect GWs with better vertical but poorer horizontal resolutions. To resolve different parts of the broad GW spectrum, both satellite limb and nadir observing techniques are needed, and a better understanding of GW complexities requires joint analyses of these data and dedicated high-resolution model simulations.     

Determination of surface global radiation using METEOSAT images and ground based visibility measurements

Our purpose is the forecast of the global radiation. As a first step we try to determine the global radiation as function of other predictable parameters. First the daily average values of the relative global radiation was considered as parabolic function of the cloud coverage obtained from METEOSAT images, an empirical formula was determined for calculation of the relative global radiation from the cloud amount. The correct this formula the cloud coverage and ground based visibility data were used. The value of the multiple correlation coefficient presenting the accuracy of the new formula was 0.96 for Budapest, and 0.93 for region of Hungary. This fact indicates that we have a sufficiently correct formula for calculation of the global radiation.     

Two-component Bénard convection: Interfacial deformation,oscillatory instabilities and the onset of turbulence

Instabilities and convection in binary fluid mixtures (liquids with impurities, binary gas mixtures, etc.) have been the subject of intense research over the past decade. The major finding has been that mere ppm impurity concentration can play a drastic role in the stability of fluid layers subjected to thermal gradients. For layers open to ambient air the impurity cooperates or competes according to the sign of its migration in the thermal fields with the interfacial deformation thus leading to convective flows. For a layer enclosed between copper plates, say, the impurity can interplay with the slightest buoyancy or buoyancy variations in the environment thus amplifying this force and bringing instability under circumstances where with pure fluids no instability is expected. Recent predictions reported here refer to oscillatory instabilities with or without interfacial deformation and the onset of turbulence in the form of chaotic flows which correspond to irregular patterns with decaying correlations and broadband noise in their power spectra.     

基于模态分析的配电产品耐振性优化设计

振动是导致机载电子设备损坏的重要载荷,线路板部件是电子设备的核心部件。在配电产品的设计 阶段,需要对线路板进行动力学分析和抗振优化设计。根据某机载配电产品的方案设计,采用 MSC.Patran和 MSC.Nastran软件对产品结构进行模态分析、振动分析,得到产品的固有频率及振动响应曲线;基于模态分析, 提出不同的优化方案,并进行建模及模态分析。结果表明:通过增加线路板厚度、合理布局线路板上的元器件、 对加强筋进行翻边处理以及在较易发生变形的部位增加螺钉约束等手段,可以提高产品的耐振性。     

Validation of the in-flight calibration procedures for the MICROSCOPE space mission

The MICROSCOPE space mission aims to test the Equivalence Principle with an accuracy of 10^-15${10}^{-15}$. The drag-free micro-satellite will orbit around the Earth and embark a differential electrostatic accelerometer including two cylindrical test masses submitted to the same gravitational field and made of different materials. The experience consists in testing the equality of the electrostatic acceleration applied to the masses to maintain them relatively motionless. The accuracy of the measurements exploited for the test of the Equivalence Principle is limited by our a priori knowledge of several physical parameters of the instrument. These parameters are partially estimated on-ground, but with an insufficient accuracy, and an in-orbit calibration is therefore required to correct the measurements. The calibration procedures have been defined and their analytical performances have been evaluated. In addition, a simulator software including the dynamics model of the instrument, the satellite drag-free system and the perturbing environment has been developed to numerically validate the analytical results. After an overall presentation of the MICROSCOPE mission, this paper will describe the calibration procedures and focus on the simulator. Such an in-flight calibration is mandatory for similar space missions taking advantage of a drag-free system.     

Wireless sensor networks for planetary exploration: Experimental assessment of communication and deployment

Planetary surface exploration is an appealing application of wireless sensor networks that has been investigated in recent years by the space community, including the European Space Agency. The idea is to deploy a number of self-organizing sensor nodes forming a wireless networked architecture to provide a distributed instrument for the study and exploration of a planetary body. To explore this concept, ESA has funded the research project RF Wireless for Planetary Exploration (RF-WIPE), carried out by GMV, SUPSI and UPM. The purpose of RF-WIPE was to simulate and prototype a wireless sensor network in order to assess the potential and limitations of the technology for the purposes of planetary exploration.