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291.
Exposure of fungal conidia (Aspergillus ochraceus) or spores of Bacillus subtilis to extreme dryness or vacuum induces DNA lesions, including strand breaks and the formation of DNA-protein cross-links. In wet cells only a small amount of protein is bound to DNA, but exposure to conditions of lowered water activity results in an increasing number of cross-links between DNA and proteins. In fungal conidia these cross-links are detected after selective iodination (125 J) of the DNA-bound proteins followed by gel electrophoresis and subsequent autoradiography. Another approach is the labelling of DNA with 32P by means of nick translation and the detection of differences in the electrophoretic mobility of DNA before and after digestion with proteinase K of proteins bound to DNA.  相似文献   
292.
We investigated the effect of substratum adhesiveness on stimulated lymphocyte blastogenesis by reducing and blocking cell adhesion with poly (2-hydroxyethyl methacrylate) (poly-HEMA) in a simple on-ground system. Cells grown on medium-thick and thick poly-HEMA films were rounded in shape and displayed no signs of spreading. By contrast, on tissue culture plastic and very thin poly-HEMA films, they showed clear signs of spreading. The mitogenic response of lymphocytes grown on thick poly-HEMA films was reduced by up to 68% of the control (tissue culture plastic). Interferon-gamma production was near zero when the cells were grown on the least adhesive substratum. On uncoated plastic, activated lymphocytes subjected to high gravity (20g) exhibited an increased proliferation rate (40%) compared with 1g. By contrast, on poly-HEMA, high gravity did not improve lymphocyte responsiveness. These results show that activated lymphocytes need to anchor and spread prior to achieving an optimal proliferation response. We conclude that decreased lymphocyte adhesion could contribute to the depressed in vitro lymphocyte responsiveness found in the microgravity conditions of space flight.  相似文献   
293.
The Solwind coronagraph on the P78-1 earth-orbiting satellite has been monitoring the Sun routinely at 10-minute intervals during the 5-year interval from April, 1979 to the present. In a statistical analysis of about 1000 mass ejections observed through the end of 1981, we find an average occurrence rate of 1.8 mass ejections per day. Histograms of speed, central latitude, angular span, brightness, and other parameters have been constructed, and properties such as shape classification have been tabulated. These characteristics are summarized for these years near sunspot maximum. The average speed and mass estimate are found to be similar to those found at the declining phase of the previous sunspot cycle. The angular span and central latitude distributions are quite different than seen during the declining phase, and are very dependent upon structural class. The fluctuations in the occurrence rate of CMEs does not seem to match the fluctuations in the sunspot number. There is a tendency for high speed CMEs to occur more frequently in 1981 than in 1980, and more frequently in 1980 than in 1979.  相似文献   
294.
The CORONAS-F mission experiments and results have been reviewed. The observations with the DIFOS multi-channel photometer in a broad spectral range from 350 to 1500 nm have revealed the dependence of the relative amplitudes of p-modes of the global solar oscillations on the wavelength that agrees perfectly well with the earlier data obtained in a narrower spectral ranges. The SPIRIT EUV observations have enabled the study of various manifestations of solar activity and high-temperature events on the Sun. The data from the X-ray spectrometer RESIK, gamma spectrometer HELICON, flare spectrometer IRIS, amplitude–temporal spectrometer AVS-F, and X-ray spectrometer RPS-1 have been used to analyze the X- and gamma-ray emission from solar flares and for diagnostics of the flaring plasma. The absolute and relative content of various elements (such as potassium, argon, and sulfur) of solar plasma in flares has been determined for the first time with the X-ray spectrometer RESIK. The Solar Cosmic Ray Complex monitored the solar flare effects in the Earth’s environment. The UV emission variations recorded during solar flares in the vicinity of the 120-nm wavelength have been analyzed and the amplitude of relative variations has been determined.  相似文献   
295.
    
A multiple-beam satellite communications system that can adaptbeam power to varying beam traffic is proposed. A new type active-array fed, single reflector antenna design and an S band, 5-beamsystem concept including interbeam exchange are described. Basedon these concepts, a high EIRP multiple-beam system with trafficadaptability similar to a single-beam system is realized.  相似文献   
296.
    
A wide variety of organisms (the so-called \"anhydrobiotes') is able to survive long periods of time in a state of utmost dehydration and can thus survive in extremely dry environments including artificially imposed or space vacuum. Known strategies of survival include the accumulation of certain polyols, especially disaccharides, which help prevent damage to membranes and proteins. Here we report that DNA in vacuum-dried spores is damaged to a very substantial degree by processes leading to DNA strand breaks. Most of these lesions are obviously repaired during germination, but extensive damage to DNA and enzymes after long exposure times (months to years) finally diminish the chances of survival.  相似文献   
297.
    
The Cassini-Huygens Cosmic Dust Analyzer (CDA) is intended to provide direct observations of dust grains with masses between 10−19 and 10−9 kg in interplanetary space and in the jovian and saturnian systems, to investigate their physical, chemical and dynamical properties as functions of the distances to the Sun, to Jupiter and to Saturn and its satellites and rings, to study their interaction with the saturnian rings, satellites and magnetosphere. Chemical composition of interplanetary meteoroids will be compared with asteroidal and cometary dust, as well as with Saturn dust, ejecta from rings and satellites. Ring and satellites phenomena which might be effects of meteoroid impacts will be compared with the interplanetary dust environment. Electrical charges of particulate matter in the magnetosphere and its consequences will be studied, e.g. the effects of the ambient plasma and the magnetic field on the trajectories of dust particles as well as fragmentation of particles due to electrostatic disruption.The investigation will be performed with an instrument that measures the mass, composition, electric charge, speed, and flight direction of individual dust particles. It is a highly reliable and versatile instrument with a mass sensitivity 106 times higher than that of the Pioneer 10 and 11 dust detectors which measured dust in the saturnian system. The Cosmic Dust Analyzer has significant inheritance from former space instrumentation developed for the VEGA, Giotto, Galileo, and Ulysses missions. It will reliably measure impacts from as low as 1 impact per month up to 104 impacts per second. The instrument weighs 17 kg and consumes 12 W, the integrated time-of-flight mass spectrometer has a mass resolution of up to 50. The nominal data transmission rate is 524 bits/s and varies between 50 and 4192 bps.This revised version was published online in July 2005 with a corrected cover date.  相似文献   
298.
针对雷达目标观测和处理在不同的坐标系下完成,本文提出了联合滤波算法来跟踪机动目标。该算法以卡尔曼滤波器为基础,直角坐标系下和极坐标系下的算法相联合,不仅克服了两种坐标系下滤波算法的不足,而且对机动目标有很好的跟踪效果。仿真实验结果表明了该算法的有效性。  相似文献   
299.
    
Daily Be-7 concentrations in air at the height of 15 m are continuously observed at 38°15.2′N, 140°20.9′E, between 2000 and 2001. The average concentration and the relative standard deviation were 4.0 mBq/m3 and 50% in 2000–2001, respectively. The Be-7 concentrations increased 2.5% with the decrease in the sunspot numbers by 6.7% for the term of two years. From the power spectral analysis, the periodicity of 26 days is shown for the daily Be-7 concentrations. The folding analysis indicates that the time variation of the Be-7 concentration is similar to that of the ground-based neutron counting rate, and the phase delay for the minimum portion of Be-7 concentration was roughly 8 days to the maximum sunspot number. These results indicate that the Be-7 concentrations in the air at ground level have 26 day periodicity as a component of time variations and the time variation is caused by the solar modulation of galactic cosmic rays, which corresponds to the variation of the sunspot number due to the rotation of the sun.  相似文献   
300.
A modification of the Doppler Interferometry Technique is suggested to enable estimating angles of arrival of comparatively broadband HF signals scattered by random irregularities of the ionospheric plasma with the use of small-size weakly directional antennas. The technique is based on the measurements of cross-spectra phases of the probe radiation recorded at least in three spatially separated points. The developed algorithm has been used to investigate the angular and frequency-time characteristics of HF signals propagating at frequencies above the maximum usable one (MUF) for the direct radio path Moscow-Kharkiv. The received signal spectra show presence of three families of spatial components attributed, respectively, to scattering by plasma irregularities near the middle point of the radio path, ground backscatter signals and scattering of the sounding signals by the intense plasma turbulence associated with auroral activations. It has been shown that the regions responsible for the formation of the third family components are located well inside the auroral oval. The drift velocity and direction of the auroral ionosphere plasma have been determined. The obtained estimates are consistent with the classical conception of the ionospheric plasma convection at high latitudes and do not contradict the results of investigations of the auroral ionosphere dynamics using the SuperDARN network.  相似文献   
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