Hypergravity-induced changes in gene expression in Arabidopsis hypocotyls.

Under hypergravity conditions, the cell wall of stem organs becomes mechanically rigid and elongation growth is suppressed, which can be recognized as the mechanism for plants to resist gravitational force. The changes in gene expression by hypergravity treatment were analyzed in Arabidopsis hypocotyls by the differential display method, for identifying genes involved in hypergravity-induced growth suppression. Sixty-two cDNA clones were expressed differentially between the control and 300 g conditions: the expression levels of 39 clones increased, whereas those of 23 clones decreased under hypergravity conditions. Sequence analysis and database searching revealed that 12 clones, 9 up-regulated and 3 down-regulated, have homology to known proteins. The expression of these genes was further analyzed using RT-PCR. Finally, six genes were confirmed to be up-regulated by hypergravity. One of such genes encoded 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (HMGR), which catalyzes a reaction producing mevalonic acid, a key precursor of terpenoids such as membrane sterols and several types of hormones. The expression of HMGR gene increased within several hours after hypergravity treatment. Also, compactin, an inhibitor of HMGR, prevented hypergravity-induced growth suppression, suggesting that HMGR is involved in suppression of Arabidopsis hypocotyl growth by hypergravity. In addition, hypergravity increased the expression levels of genes encoding CCR1 and ERD15, which were shown to take part in the signaling pathway of environmental stimuli such as temperature and water, and those of the alpha-tubulin gene. These genes may be involved in a series of cellular events leading to growth suppression of stem organs under hypergravity conditions.     

Variation of foF2 with solar activity

Ionospheric hourly monthly-median values of the F2-layer critical frequency, foF2, from six European stations are correlated with the corresponding 12-month running mean values of each of the six solar indices, the Zurich sunspot number R, the solar radio noise flux at 10.7 cm F, the ionospheric index of solar activity IF2, the index IG, the Australian T index and the Russian RS ionospheric index, using various models. The statistical analysis shows that there is no difference in the degree of correlation in using one index over another. Their statistical behaviour is virtually identical. Furthermore, it is shown that there is a slight degree of favourability for a quadratic relation between foF2 and any index of solar activity.     

Bayesian gamma mixture model approach to radar target recognition

This paper develops a Bayesian gamma mixture model approach to automatic target recognition (ATR). The specific problem considered is the classification of radar range profiles (RRPs) of military ships. However, the approach developed is relevant to the generic discrimination problem. We model the radar returns (data measurements) from each target as a gamma mixture distribution. Several different motivations for the use of mixture models are put forward, with gamma components being chosen through a physical consideration of radar returns. Bayesian formalism is adopted and we obtain posterior distributions for the parameters of our mixture models. The distributions obtained are too complicated for direct analytical use in a classifier, so Markov chain Monte Carlo (MCMC) techniques are used to provide samples from the distributions. The classification results on the ship data compare favorably with those obtained from two previously published techniques, namely a self-organizing map and a maximum likelihood gamma mixture model classifier.     

Capability of Bhaskara-II satellite microwave radiometer brightness temperature data to discriminate soil moisture conditions of Indian landmass

With the objective of developing Microwave Remote Sensing technology in the country, India has launched a series of Satellites Bhaskara-I and II with the microwave radiometer capability. In this paper, an attempt is made to demonstrate the capability of the brightness temperature data acquired by these radiometers to discriminate various soil moisture conditions of Indian land mass. The analysis show that large areas assessment of soil moisture is possible to a limited extent.     

Reliability comparison of matrix and other converter topologies

Several rectifier-inverter and matrix converter topologies suitable for aerospace applications are compared, and their reliability is predicted. The military handbook MIL-HDBK-217F guidelines have been used to predictreliability. The matrix converter has several attractive features for aerospace applications such as potential size and weight savings. Although the matrix converter has a higher number of semiconductor switches, they are subjected to a lower voltage stress, which decreases their failure rate. This results in the reliability indicators of the different converter topologies being very similar     

IMF Direction Derived from Cycloid-Like Ion Distributions Observed by Mars Express

Although the Mars Express (MEX) does not carry a magnetometer, it is in principle possible to derive the interplanetary magnetic field (IMF) orientation from the three dimensional velocity distribution of pick-up ions measured by the Ion Mass Analyser (IMA) on board MEX because pick-up ions' orbits, in velocity phase space, are expected to gyrate around the IMF when the IMF is relatively uniform on a scale larger than the proton gyroradius. During bow shock outbound crossings, MEX often observed cycloid distributions (two dimensional partial ring distributions in velocity phase space) of protons in a narrow channel of the IMA detector (only one azimuth for many polar angles). We show two such examples. Three different methods are used to derive the IMF orientation from the observed cycloid distributions. One method is intuitive (intuitive method), while the others derive the minimum variance direction of the velocity vectors for the observed ring ions. These velocity vectors are selected either manually (manual method) or automatically using simple filters (automatic method). While the intuitive method and the manual method provide similar IMF orientations by which the observed cycloid distribution is well arranged into a partial circle (representing gyration) and constant parallel velocity, the automatic method failed to arrange the data to the degree of the manual method, yielding about a 30° offset in the estimated IMF direction. The uncertainty of the derived IMF orientation is strongly affected by the instrument resolution. The source population for these ring distributions is most likely newly ionized hydrogen atoms, which are picked up by the solar wind.     

Variation of the orbital elements for parabolic trajectories due to a small impulse using Gauss equations

Firstly we derive Gauss’ perturbation equation for parabolic motion using Murray–Dermott and Kovalevsky procedures. Secondly, we easily deduce the variations of the orbital elements for the parabolic trajectories due to a small impulse at any point along the path and at the vertex of the parabola.     

X-ray spectral properties of non-magnetic cataclysmic variables using Chandra HETG

Results from a study of high resolution spectra obtained with the Chandra X-ray observatory for a sample of 6 Cataclysmic Variables (CVs) are presented. A global fit approach has been employed to obtain the spectral characteristics of the sources. The line-rich high-resolution spectra of these sources clearly indicate multi-temperature nature of the emitting plasma. Multi-temperature APEC models describe the spectra very well. Detection of significantly broad emission lines, indicates the presence of high velocity gas in SS Cyg and U Gem during the optical outbursts.     

CNS development under altered gravity: cerebellar glial and neuronal protein expression in rat neonates exposed to hypergravity.

The future of space exploration depends on a solid understanding of the developmental process under microgravity, specifically in relation to the central nervous system (CNS). We have previously employed a hypergravity paradigm to assess the impact of altered gravity on the developing rat cerebellum. The present study addresses the molecular mechanisms involved in the cerebellar response to hypergravity. Specifically, the study focuses on the expression of selected glial and neuronal cerebellar proteins in rat neonates exposed to hypergravity (1.5 G) from embryonic day (E)11 to postnatal day (P)6 or P9 (the time of maximal cerebellar changes) comparing them against their expression in rat neonates developing under normal gravity. Proteins were analyzed by quantitative Western blots of cerebellar homogenates; RNA analysis was performed in the same samples using quantitative PCR. Densitometric analysis of Western blots suggested a reduction in glial (glial acidic protein, GFAP) and neuronal (neuronal cell adhesion molecule, NCAM-L1, synaptophysin) proteins, but the changes in individual cerebellar proteins in hypergravity-exposed neonates appeared both age- and gender-specific. RNA analysis suggested a reduction in GFAP and synaptophysin mRNAs on P6. These data suggest that exposure to hypergravity may interfere with the expression of selected cerebellar proteins. These changes in protein expression may be involved in mediating the effect of hypergravity on the developing rat cerebellum.