High phosphorylase activity is correlated with increased potato minituber formation and starch content during extended clinorotation.

The major purpose of these experiments were to investigate growth of potato storage organs and starch synthesis in minitubers at slow horizontal clinorotation (2 rpm), which partly mimics microgravity, and a secondary goal was to study the activity and localization of phosphorylase (EC 2.4.1.1) in storage parenchyma under these conditions. Miniplants of Solanum tuberosum L. (cv Adreta) were grown in culture for 30 days for both the vertical control and the horizontal clinorotation. During long-term clinorotation, an acceleration of minituber formation, and an increase of amyloplast number and size in storage parenchyma cells, as well as increased starch content, was observed in the minitubers. The differences among cytochemical reaction intensity, activity of phosphorylase, and carbohydrate content in storage parenchyma cells of minitubers grown in a horizontal clinostat were established by electron-cytochemical and biochemical methods. It is shown that high phosphorylase activity is correlated with increased starch content during extended clinorotation. The results demonstrate the increase in carbohydrate metabolism and possible accelerated growth of storage organs under the influence of microgravity, as mimicked by clinorotation; therefore, clinorotation can be used as a basis for future studies on mechanisms of starch synthesis under microgravity.     

Effects of RF absorbers on measurements of small antennas in smallanechoic chambers

In this work, the influence of resistive absorbers in the vicinity of small antennas was investigated, in order to define a lower limit for the size of a small anechoic chamber for antenna calibration and radiation pattern measurements, with respect to the distance between the antenna under test (AUT) and the RF absorbers lining the walls. Furthermore, the influence of a relatively narrow transmission channel, formed by the absorbers on the side walls between the measurement antenna and the AUT was investigated. It was found by measurements that absorbers with the length of 610 mm do not have a measurable effect on the radiation characteristics of a small antenna at around 1.8 GHz, as long as they are kept more than 100 mm from the AUT, and the transmission channel formed by the absorbers between the antennas is wider than 100 mm     

Observations of Magnetospheric Waves and their Relation to Precipitation

Magnetospheric wave observations are discussed from the viewpoint of their potential importance for precipitation of charged particles into the auroral zones. While wave processes are a fundamental part of magnetospheric plasma physics, occurring most of the time in most of the magnetospheric regions, their direct role in and relative importance for auroral precipitation are not easy to assess. The role of the waves varies from one spatial region to another and is very different for electrons and ions. Furthermore, the distinction between wave processes and other precipitation mechanisms is not at all straightforward. This review focuses on four main topics: The problem of diffuse electron precipitation, the recent surprise on the detailed structure of broad-banded electrostatic noise in the plasma sheet boundary layer, ion precipitation through electromagnetic ion cyclotron waves, and the role of low-altitude waves in precipitation. It is concluded that, while the observational status of high-altitude ion cyclotron waves is reasonably good, in most areas more thorough studies of existing data as well as refined observations are very much needed. Successful observational studies are to be carried out jointly with theoretical work as well as with studies on the large-scale context of the often localized wave processes. This is especially important when interests are moving toward more nonlinear phenomena, such as shocks, double layers, or strong quasi-static gradients, where a strict adherence to classical wave concepts is becoming more and more diffuse and less motivated.     

Radiation biodosimetry: applications for spaceflight.

The multiparametric dosimetry system that we are developing for medical radiological defense applications could be adapted for spaceflight environments. The system complements the internationally accepted personnel dosimeters and cytogenetic analysis of chromosome aberrations, considered the best means of documenting radiation doses for health records. Our system consists of a portable hematology analyzer, molecular biodosimetry using nucleic acid and antigen-based diagnostic equipment, and a dose assessment management software application. A dry-capillary tube reagent-based centrifuge blood cell counter (QBC Autoread Plus, Becton [correction of Beckon] Dickinson Bioscience) measures peripheral blood lymphocytes and monocytes, which could determine radiation dose based on the kinetics of blood cell depletion. Molecular biomarkers for ionizing radiation exposure (gene expression changes, blood proteins) can be measured in real time using such diagnostic detection technologies as miniaturized nucleic acid sequences and antigen-based biosensors, but they require validation of dose-dependent targets and development of optimized protocols and analysis systems. The Biodosimetry Assessment Tool, a software application, calculates radiation dose based on a patient's physical signs and symptoms and blood cell count analysis. It also annotates location of personnel dosimeters, displays a summary of a patient's dosimetric information to healthcare professionals, and archives the data for further use. These radiation assessment diagnostic technologies can have dual-use applications supporting general medical-related care.     

Ultracapacitors + DC-DC converters in regenerative braking system

An ultracapacitor system for an electric vehicle has been implemented. The device allows higher accelerations and decelerations of the vehicle with minimal loss of energy and minimal degradation of the main battery pack. The system uses a DC-DC power converter, which is connected between the ultracapacitor and the main battery pack. The design has been optimized in weight and size, by using water-cooled heat sinks for the power converter, and an aluminum coil with air core for the smoothing inductance. The ratings of the ultracapacitor are: nominal voltage: 300 Vdc; nominal current: 200 Adc; capacitance: 20 Farads. The amount of energy stored allows us to have 40 kW of power during 20 seconds, which is enough to accelerate the vehicle without the help of the traction batteries. The vehicle uses a brushless DC motor with a nominal power of 32 kW and a peak power of 53 kW. A control system based on a Digital Signal Processor (DSP) manipulates all the aforementioned variables and controls the Pulse Width Modulation (PWM) switching pattern of the converter transistors. The car used for the implementation of this system is a Chevrolet LUV truck.     

Development of a Digital Array Radar (DAR)

Twenty-first century littoral and open-sea missions present US Navy (USN) shipboard-radar systems with the challenge of detecting small targets in severe clutter and against multiple sources of interference. In Fiscal Year 2000 (FY00), the Office of Naval Research (ONR) sponsored a program to develop an active array radar that includes a digital beamforming (DBF) architecture. The DBF radar system has the potential for improved time-energy management, improved signal-to-clutter (S/C) ratios, improved reliability and reduced life-cycle costs. This paper summarizes the latest developments of the program during FY00     

System identification of dynamic closed-loop control of total peripheral resistance by arterial and cardiopulmonary baroreceptors.

Prolonged exposure to microgravity in space flight missions (days) impairs the mechanisms responsible for defense of arterial blood pressure (ABP) and cardiac output (CO) against orthostatic stress in the post-flight period. The mechanisms responsible for the observed orthostatic intolerance are not yet completely understood. Additionally, effective counter measures to attenuate this pathophysiological response are not available. The aim of this study was to investigate the ability of our proposed system identification method to predict closed-loop dynamic changes in TPR induced by changes in mean arterial pressure (MAP) and right atrial pressure (RAP). For this purpose we designed and employed a novel experimental animal model for the examination of arterial and cardiopulmonary baroreceptors in the dynamic closed-loop control of total peripheral resistance (TPR), and applied system identification to the analysis of beat-to-beat fluctuations in the measured signals. Grant numbers: NAG5-4989.     

Energy storage at 77 K in multilayer ceramic capacitors

A ceramic material having a large dielectric constant at 77 K, ε=8000-12000, has been developed for capacitive energy storage at this temperature. A large matrix of multilayer ceramic capacitors were fabricated using conventional tape-casting methods to optimize the dielectric breakdown strength at 77 K, and measured energy storage values on these capacitors range up to 6 J/cm³ at 77 K. An unfused bank of these capacitors was voltage-cycled 10⁵ times at 77 K without failure, and the heating effects during cycling were immeasurably small (i.e., nitrogen boiloff was monitored). An electrocaloric effect on discharge (ΔT~1 K) contributes to the thermal stability. Measurements of the frequency dependence of the dielectric properties of the ceramic at 77 K indicate a fundamental limit of about 8 μs for the switching repetition rate. Improved capacitor-manufacturing methods are discussed which can increase the energy density to the 20-30 J/cm³ range