Distribution of calmodulin in corn seedlings: Immunocytochemical localization in coleoptiles and root apices

Immunofluorescence techniques have been used to study the distribution of calmodulin in several tissues in etiolated corn ( , var. Bear Hybrid) seedlings. Uniform staining was seen in the background cytoplasm of most cell types. Cell walls and vacuoles were not stained. In coleoptile mesophyll cells the nucleoplasm of most nuclei was stained as was the stroma of most amyloplasts. The lumen border of mature tracheary elements in coleoptiles also stained. In the rootcap the most intensely stained regions were the cytoplasms of columella cells and of the outermost cells enmeshed in the layer of secreted slime. Nuclei in the rootcap cells did not stain distinctly, but those in all cell types of the root meristem did. Also in the root meristem, the cytoplasm of metaxylem elements stained brightly. These results are compared and contrasted with previous data on the localization of calmodulin in pea root apices and epicotyls and discussed in relation to current hypotheses on mechanisms of gravitropism.     

Theoretical consideration of the chemical pathways for radiation-induced strand breaks.

A theoretical approach to the understanding of the biochemical mechanisms of indirect action of ionizing radiation on SV40 DNA in aqueous solution is presented. The extent of OH attack on the sugar moiety and bases has been calculated. A realistic model for the DNA (in B form) based on available X-ray diffraction data is used and specific reaction sites for the OH radicals are obtained. A Monte Carlo scheme is used to follow the diffusion and reaction of the OH radicals. Effects of track structure have been considered and the single strand break D37 values for 14 MeV electrons (low-LET) and 670 MeV/u and 40 MeV/u neon particles are presented. Calculated results are in agreement with available experimental data. It has been found that regardless of the qualities of radiation, 80% of the OH attack on DNA is on the bases and 20% is on the deoxyribose. From probability considerations only, it appears that the number of double strand breaks varies linearly with dose.     

Growth and development of cultured carrot cells and embryos under spaceflight conditions

Morphogenetically competent proembryonic cells and well-developed somatic embryos of carrot at two levels of organization were exposed for 18.5 days to a hypogravity environment aboard the Soviet Biosatellite Cosmos 1129. It was confirmed that cultured totipotent cells of carrot can give rise to embryos with well-developed roots and minimally developed shoots. It was also shown that the space hypogravity environment could support the further growth of already-organized, later somatic embryonic stages and give rise to fully developed embryo-plantlets with roots and shoots.     

Low energy observations of Cygnus X-2 by Ariel VI

Ariel VI observations of Cygnus X-2 have revealed a rather flat spectrum between 0.1 and 1.5 keV with variable emission at low energy. Of the two conflicting interpretations of this object in terms of i) a distant high-luminosity (L_x 10³⁸ ergs s⁻¹) binary and ii) a nearby low-luminosity (L_x 10³⁵ ergs s⁻¹) degenerate dwarf system, our measurements support the latter.     

Analysis of the physical conditions in a strong X-ray flare

The temperature distribution of the hot plasma emission measure in a large but slowly developing flare has been investigated using the following data obtained from the INTERCOSMOS 4 satellite: (1) the X - ray spectra in the range 1.7 – 1.9 Å, (2) the hard X - ray fluxes in the range 10 – 40 keV. It has been found that all the data can be explained by a consistent thermal model of the emitting region.     

Recurrent isolation of hydrogen peroxide-resistant spores of Bacillus pumilus from a spacecraft assembly facility

While the microbial diversity of a spacecraft assembly facility at the Jet Propulsion Laboratory (Pasadena, CA) was being monitored, H2O2-resistant bacterial strains were repeatedly isolated from various surface locations. H2O2 is a possible sterilant for spacecraft hardware because it is a low-temperature process and compatible with various modern-day spacecraft materials, electronics, and components. Both conventional biochemical testing and molecular analyses identified these strains as Bacillus pumilus. This Bacillus species was found in both unclassified (entrance floors, anteroom, and air-lock) and classified (floors, cabinet tops, and air) locations. Both vegetative cells and spores of several B. pumilus isolates were exposed to 5% liquid H2O2 for 60 min. Spores of each strain exhibited higher resistance than their respective vegetative cells to liquid H2O2. Results indicate that the H2O2 resistance observed in both vegetative cells and spores is strain-specific, as certain B. pumilus strains were two to three times more resistant than a standard Bacillus subtilis dosimetry strain. An example of this trend was observed when the type strain of B. pumilus, ATCC 7061, proved sensitive, whereas several environmental strains exhibited varying degrees of resistance, to H2O2. Repeated isolation of H2O2-resistant strains of B. pumilus in a clean-room is a concern because their persistence might potentially compromise life-detection missions, which have very strict cleanliness and sterility requirements for spacecraft hardware.     

A search for chiral signatures on Mars

It is thought that the chiral molecules of living material can induce circular polarization in light at levels much higher than expected from abiotic processes. We therefore obtained high quality imaging circular polarimetry of the martian surface during the favorable opposition of 2003 to seek evidence of anomalous optical activity. We used two narrow-band filters covering 43% of the martian surface, 15% of it in-depth. With polarization noise levels <0.1% (4.3 upper limits 0.2-0.3%) and spatial resolution 210 km, we did not find any regions of circular polarization. When data were averaged over the observed face of the planet, we did see a small non-zero circular polarization 0.02%, which may be due to effects associated with the opposition configuration though it is at the limit of the instrumental capability. Our observations covered only a small fraction of parameter space, so although we obtained a null result, we cannot exclude the presence of optical activity at other wavelengths, in other locations, or at higher spatial resolution.     

Loading effects on rat craniomandibular morphology: a system for gravity studies

Gravity effects on muscle and bone are a major impediment to long-term space travel. We introduce a model for studying these effects, the craniomandibular system. Some advantages of this system include: (1) craniomandibular morphology is determined by epigenetic factors including gravity, (2) relatively light forces can significantly alter its morphology, and (3) soft diet and tooth loss produce effects that are similar to those produced in lower limbs by weightlessness. In the study, implants made either of gold (experimental group) or lightweight acrylic (controls) were attached to adult rats' mandibles. After 13 weeks, the animals' skulls and mandibles were dissected. Pair-wise comparisons indicated that the experimental animals showed significantly shortened and narrowed cranial bases, and significant changes in the posterior zygomatic arch region. These results indicate that simulated macrogravity influences bone remodeling in the adult craniomandibular system.     

Vanguard--a European robotic astrobiology-focussed Mars sub-surface mission proposal

We present a new European Mars mission proposal to build on the UK-led Beagle2 Mars mission and continue its astrobiology-focussed investigation of Mars. The small surface element to be delivered to the Martian surface--Vanguard--is designed to be carried by a Mars Express-type spacecraft bus to Mars and adopts a similar entry, descent and landing system as Beagle2. The surface element comprises a triad of robotic devices--a lander, a micro-rover of the Sojourner class for surface mobility, and three ground-penetrating moles mounted onto the rover for sub-surface penetration to 5 m depth. The major onboard instruments on the rover include a Raman spectrometer/imager, a laser plasma spectrometer, an infrared spectrometer--these laser instruments provide the basis for in situ "remote" sensing of the sub-surface Martian environment within a powerful scientific package. The moles carry the instruments' sensor head array to the sub-surface. The moles are thus required to undergo a one-way trip down the boreholes without the need for recovery of moles or samples, eliminating much of the robotic complexity invoked by such operations.     

Effect of variable thermal properties of the solid phase on composite solid propellant combustion

Effects of variable thermal properties (specific heat and thermal conductivity) of the solid phase on the combustion of composite solid propellants are studied analytically. Both steady and unsteady burning are considered. It is shown that the effects of variable thermal properties are small and can be accounted for by choosing proper average values of specific heat and thermal conductivity.