LET dependence of cell death, mutation induction and chromatin damage in human cells irradiated with accelerated carbon ions.

We investigated the LET dependence of cell death, mutation induction and chromatin break induction in human embryo (HE) cells irradiated by accelerated carbon-ion beams. The results showed that cell death, mutation induction and induction of non-rejoining chromatin breaks detected by the premature chromosome condensation (PCC) technique had the same LET dependence. Carbon ions of 110 to 124keV/micrometer were the most effective at all endpoints. However, the number of initially induced chromatin breaks was independent of LET. About 10 to 15 chromatin breaks per Gy per cell were induced in the LET range of 22 to 230 keV/micrometer. The deletion pattern of exons in the HPRT locus, analyzed by the polymerase chain reaction (PCR), was LET-specific. Almost all of the mutants induced by 124 keV/micrometer beams showed deletion of the entire gene, while all mutants induced by 230keV/micrometer carbon-ion beams showed no deletion. These results suggest that the difference in the density distribution of carbon-ion track and secondary electron with various LET is responsible for the LET dependency of biological effects.     

Protein requirements for long term missions.

A key component of the diet for a space mission is protein. This first part of this paper reviews the reasons for emphasizing protein nutrition and then discusses what the requirements are likely to be. The second part discusses potential advantages of modifying these requirements and describes potential approaches to effecting these modifications based on well established ground based models.     

The ASTROCULTURE(TM) flight experiment series, validating technologies for growing plants in space.

A flight experiment, ASTROCULTURE(TM)-1 (ASC-1), to evaluate the operational characteristics and hardware performance of a porous tube nutrient delivery system (PTNDS) was flown on STS-50 as part of the U.S. Microgravity Laboratory-1 mission, 25 June to 9 July, 1992. This experiment is the first in a series of planned ASTROCULTURE(TM) flights to validate the performance of subsystems required to grow plants in microgravity environments. Results indicated that the PTNDS was capable of supplying water and nutrients to plants in microgravity and that its performance was similar in microgravity to that in 1g on Earth. The data demonstrated that water transfer rates through a rooting matrix are a function of pore size of the tubes, the degree of negative pressure on the 'supply' fluid, and the pressure differential between the 'supply' and 'recovery' fluid loops. A slightly greater transfer rate was seen in microgravity than in 1g, but differences were likely related to the presence of hydrostatic pressure effects at 1g. Thus, this system can be used to support plant growth in microgravity or in partial gravity as on a lunar or Mars base. Additional subsystems to be evaluated in the ASTROCULTURE(TM) flight series of experiments include lighting, humidity control and condensate recovery, temperature control, nutrient composition control, CO2 and O2 control, and gaseous contaminant control.     

Beginner's guide to neural networks

This guide introduces the basics of neural networks and is intended to prepare the reader to search, read, use, and understand the literature. A brief history is followed by a section devoted to keywords, phrases, and definitions. The basic building blocks with illustrations are included. A matrix of major neural-network paradigms with intended use and suggested applications is presented. Recommended readings and software programs with problem types are suggested. The potentials and limitations of neural networks are highlighted     

Modeling of the radiation exposure during the flight of the second Bulgarian cosmonaut on board the Mir Space Station.

An experiment involving active detection of space radiation was carried out in the Space Research Institute (SRI) of Bulgarian Academy of Sciences, in preparation of the flight of the second Bulgarian cosmonaut. The radiations that would be encountered on the flight were modelled including solar and galactic cosmic rays and the particle radiation in the Earth's radiation belts. The dose rate was calculated for these different radiations behind the shielding of the space station. The variations in dose rates over the period of the flight were calculated and compared with measurements made during the orbit of the Mir Space Station. The calculated and measured dose rates agreed within 15-35%.     

Reducing the risks of using Ada onboard the Space Station

The Ada programming language was chosen by NASA as the primary computer programming language for the development of new software for the US Space Station. Ada was selected based on the results of investigations coordinated through Johnson Space Center (JSC) and that resulted in the identification of a set of problems and risks associated with using software developed in Ada. Some of the specific solutions to problems identified through these investigations are described. Three areas in which Ada's use poses risks are discussed: real-time process control; the testing and verification of flight software for man-rated systems; and software error detection, identification, and recovery required in safety-critical systems     

Surface emitting lasers for avionics applications

Vertical cavity surface emitting lasers offer many features that are suitable for applications in optical interconnect, optical data bus, optical backplane, and optical signal processing. The authors describe basic device structure and fabrication techniques of GaAs-based vertical cavity surface emitting lasers and discuss key laser performance characteristics such as emission wavelength, beam pattern, threshold current, power output, quantum efficiency, and modulation response that are important to these potential applications. Results on monolithic integration of the laser with an n-channel MESFET driver are described     

The innovation process: interpreting customer requirements

In this article we argue that the interpreter role serves an important function in the innovation of new products and processes. In our survey of over 1000 United States Air Force engineers and project managers, we found that translating customer requirements into specification and statement of work form is one of the most difficult tasks to do in a program office. In analyzing the reasons for this difficulty, we address possible concerns about the nature of the requirement's definition process