SOLID3: a multiplex antibody microarray-based optical sensor instrument for in situ life detection in planetary exploration

The search for unequivocal signs of life on other planetary bodies is one of the major challenges for astrobiology. The failure to detect organic molecules on the surface of Mars by measuring volatile compounds after sample heating, together with the new knowledge of martian soil chemistry, has prompted the astrobiological community to develop new methods and technologies. Based on protein microarray technology, we have designed and built a series of instruments called SOLID (for "Signs Of LIfe Detector") for automatic in situ detection and identification of substances or analytes from liquid and solid samples (soil, sediments, or powder). Here, we present the SOLID3 instrument, which is able to perform both sandwich and competitive immunoassays and consists of two separate functional units: a Sample Preparation Unit (SPU) for 10 different extractions by ultrasonication and a Sample Analysis Unit (SAU) for fluorescent immunoassays. The SAU consists of five different flow cells, with an antibody microarray in each one (2000 spots). It is also equipped with an exclusive optical package and a charge-coupled device (CCD) for fluorescent detection. We demonstrated the performance of SOLID3 in the detection of a broad range of molecular-sized compounds, which range from peptides and proteins to whole cells and spores, with sensitivities at 1-2?ppb (ng?mL?1) for biomolecules and 10? to 103 spores per milliliter. We report its application in the detection of acidophilic microorganisms in the Río Tinto Mars analogue and report the absence of substantial negative effects on the immunoassay in the presence of 50?mM perchlorate (20 times higher than that found at the Phoenix landing site). Our SOLID instrument concept is an excellent option with which to detect biomolecules because it avoids the high-temperature treatments that may destroy organic matter in the presence of martian oxidants.     

Satellite altimetry determination of differences between mean sea-level heights and testing geopotential models

The Satellite Altimeter Data has been used for a) Determination of differences between mean ocean levels; b) Testing the accuracy of different geopotential models as regards their internal structure over the oceans.     

Relation algebras over containers and surfaces: An ontological study of a room space

Recent research in geographic information systems hasbeen concerned with the construction of algebras tomake inferences about spatial relations by embeddingspatial relations within a space in which decisionsabout compositions are derived geometrically. Wepursue an alternative approach by studying spatialrelations and their inferences in a concrete spatialscenario, a room space that contains such manipulableobjects as a box, a ball, a table, a sheet of paper,and a pen. We derive from the observed spatialproperties an algebra related to the fundamentalspatial concepts of containers and surfaces and showthat this container-surface algebra holds allproperties of Tarski's relation algebra, except forthe associativity. The crispness of the compositionscan be refined by considering the relative size of theobjects) and their roles (i.e., whether it isexplicitly known that the objects are containers orsurfaces).     

Chaotic exchange of solid material between planetary systems: implications for lithopanspermia

Abstract We examined a low-energy mechanism for the transfer of meteoroids between two planetary systems embedded in a star cluster using quasi-parabolic orbits of minimal energy. Using Monte Carlo simulations, we found that the exchange of meteoroids could have been significantly more efficient than previously estimated. Our study is relevant to astrobiology, as it addresses whether life on Earth could have been transferred to other planetary systems in the Solar System's birth cluster and whether life on Earth could have been transferred from beyond the Solar System. In the Solar System, the timescale over which solid material was delivered to the region from where it could be transferred via this mechanism likely extended to several hundred million years (as indicated by the 3.8-4.0?Ga epoch of the Late Heavy Bombardment). This timescale could have overlapped with the lifetime of the Solar birth cluster (～100-500?Myr). Therefore, we conclude that lithopanspermia is an open possibility if life had an early start. Adopting parameters from the minimum mass solar nebula, considering a range of planetesimal size distributions derived from observations of asteroids and Kuiper Belt objects and theoretical coagulation models, and taking into account Oort Cloud formation models, we discerned that the expected number of bodies with mass>10?kg that could have been transferred between the Sun and its nearest cluster neighbor could be of the order of 10(14) to 3·10(16), with transfer timescales of tens of millions of years. We estimate that of the order of 3·10(8)·l (km) could potentially be life-bearing, where l is the depth of Earth's crust in kilometers that was ejected as the result of the early bombardment. Key Words: Extrasolar planets-Interplanetary dust-Interstellar meteorites-Lithopanspermia. Astrobiology 12, 754-774.     

一种视频SAR运动目标成像算法

视频合成孔径雷达ViSAR(Video Synthetic Aperture Radar)成像模式与传统SAR成像模式不同,此模式需要持续监测目标区域.通过子孔径划分的方法将目标区域的回波划分孔径,然后针对每一个划分后的子孔径进行单独成像和配准,最后把每一帧图像进行处理形成视频,利用序列图像形成的视频去提取目标的运动信...     

企业投资股市的收益与风险

我国正处于由计划经济向市场经济转轨时期，在社会总需求相对不足情况下，分析了企业剩余资金投资于股市的可行性，并提出了减少投资风险，提高投资收益的渠道。     

Linear and circular structures of the Bohemian Massif — Comparison of satellite and geophysical data

Two structural schemes of the Bohemian Massif are presented and compared. The first one is a result of interpretation of various geophysical data and the second one is compiled on the basis of decoding of space imagery from different sources (Kosmos, Landsat etc.). Both schemes have many structural features in common, but there are diversities namely in the hierarchy of directions and regional distribution of linear structures. These problems are discussed in detail. For example, the most impressive system of structures is the NW-SE system in both schemes, whereas the NE-SW is more remarkable in the geophysical scheme; the N-S and the E-W systems are best expressed in the scheme of photolineaments. These facts are due to the genesis, age, development and dynamic characteristics of respective structural systems. The circular structural features namely those of large size are mostly remarkable in space imagery. At present, they are not distinguished genetically but only described regionally.     

Biosignatures from Earth-like planets around M dwarfs

Coupled one-dimensional photochemical-climate calculations have been performed for hypothetical Earth-like planets around M dwarfs. Visible/near-infrared and thermal-infrared synthetic spectra of these planets were generated to determine which biosignature gases might be observed by a future, space-based telescope. Our star sample included two observed active M dwarfs-AD Leo and GJ 643-and three quiescent model stars. The spectral distribution of these stars in the ultraviolet generates a different photochemistry on these planets. As a result, the biogenic gases CH4, N2O, and CH3Cl have substantially longer lifetimes and higher mixing ratios than on Earth, making them potentially observable by space-based telescopes. On the active M-star planets, an ozone layer similar to Earth's was developed that resulted in a spectroscopic signature comparable to the terrestrial one. The simultaneous detection of O2 (or O3) and a reduced gas in a planet's atmosphere has been suggested as strong evidence for life. Planets circling M stars may be good locations to search for such evidence.