Comparative survival analysis of Deinococcus radiodurans and the haloarchaea Natrialba magadii and Haloferax volcanii exposed to vacuum ultraviolet irradiation

The haloarchaea Natrialba magadii and Haloferax volcanii, as well as the radiation-resistant bacterium Deinococcus radiodurans, were exposed to vacuum UV (VUV) radiation at the Brazilian Synchrotron Light Laboratory. Cell monolayers (containing 10(5) to 10(6) cells per sample) were prepared over polycarbonate filters and irradiated under high vacuum (10(-5) Pa) with polychromatic synchrotron radiation. N. magadii was remarkably resistant to high vacuum with a survival fraction of (3.77±0.76)×10(-2), which was larger than that of D. radiodurans (1.13±0.23)×10(-2). The survival fraction of the haloarchaea H. volcanii, of (3.60±1.80)×10(-4), was much smaller. Radiation resistance profiles were similar between the haloarchaea and D. radiodurans for fluences up to 150?J m(-2). For fluences larger than 150?J m(-2), there was a significant decrease in the survival of haloarchaea, and in particular H. volcanii did not survive. Survival for D. radiodurans was 1% after exposure to the higher VUV fluence (1350?J m(-2)), while N. magadii had a survival lower than 0.1%. Such survival fractions are discussed regarding the possibility of interplanetary transfer of viable microorganisms and the possible existence of microbial life in extraterrestrial salty environments such as the planet Mars and Jupiter's moon Europa. This is the first work to report survival of haloarchaea under simulated interplanetary conditions.     

Magnetic turbulence at the magnetopause, a key problem for understanding the solar wind/ magnetosphere exchanges

According to ideal MHD, the magnetopause boundary should split the terrestrial environment in two disconnected domains: outside, the solar wind (including its shocked part, the magnetosheath), and inside, the magnetosphere. This view is at variance with the experimental data, which show that the magnetopause is not tight and that a net transfer of matter exists from the solar wind to the magnetosphere; it implies that the frozen-in condition must break down on the magnetopause, either over the whole boundary or at some points. In the absence of ordinary collisions, only short scale phenomena (temporal and/or spatial) can be invoked to explain this breakdown, and the best candidates in this respect appear to be the ULF magnetic fluctuations which show very strong amplitudes in the vicinity of the magnetopause boundary. It has been shown that these fluctuations are likely to originate in the magnetosheath, probably downstream of the quasi-parallel shock region, and that they can get amplified by a propagation effect when crossing the magnetopause. When studying the propagation across the magnetopause boundary, several effects are to be taken into account simultaneously to get reliable results: the magnetopause density gradient, the temperature effects, and the magnetic field rotation can be introduced while remaining in the framework of ideal MHD. In these conditions, the magnetopause amplification has been interpreted in term of Alfvén and slow resonances occurring in the layer. When, in addition, one takes the ion inertia effects into account, by the way of the Hall-MHD equations, the result appears drastically different: no resonance occurs, but a strong Alfvén wave can be trapped in the boundary between the point where it is converted from the incident wave and the point where it stops propagating back, i.e., the point where k _\|=0, which can exist thanks to the magnetic field rotation. This effect can bring about a new interpretation to the magnetopause transfers, since the Hall effect can allow reconnection near this particular point. The plasma transfer through the magnetopause could then be interpreted in terms of a reconnection mechanism directly driven by the magnetosheath turbulence, which is permanent, rather than due to any local instability of the boundary, for instance of the tearing type, which should be subject to an instability threshold and thus, as far as it exists, more sporadic.     

Approaches in the determination of plant nutrient uptake and distribution in space flight conditions.

The effective growth and development of vascular plants rely on the adequate availability of water and nutrients. Inefficiency in either the initial absorption, transportation, or distribution of these elements are factors which impinge on plant structure and metabolic integrity. The potential effect of space flight and microgravity conditions on the efficiency of these processes is unclear. Limitations in the available quantity of space-grown plant material and the sensitivity of routine analytical techniques have made an evaluation of these processes impractical. However, the recent introduction of new plant cultivating methodologies supporting the application of radionuclide elements and subsequent autoradiography techniques provides a highly sensitive investigative approach amenable to space flight studies. Experiments involving the use of gel based 'nutrient packs' and the radionuclides calcium-45 and iron-59 were conducted on the Shuttle mission STS-94. Uptake rates of the radionuclides between ground and flight plant material appeared comparable.     

Composition and physical properties of starch in microgravity-grown plants.

The effect of spaceflight on starch development in soybean (Glycine max L., BRIC-03) and potato (Solanum tuberosum, Astroculture-05) was compared with ground controls by biophysical and biochemical measurements. Starch grains from plants from both flights were on average 20-50% smaller in diameter than ground controls. The ratio delta X/delta rho (delta X --difference of magnetic susceptibilities, delta rho--difference of densities between starch and water) of starch grains was ca. 15% and 4% higher for space-grown soybean cotyledons and potato tubers, respectively, than in corresponding ground controls. Since the densities of particles were similar for all samples (1.36 to 1.38 g/cm3), the observed difference in delta X/delta rho was due to different magnetic susceptibilities and indicates modified composition of starch grains. In starch preparations from soybean cotyledons (BRIC-03) subjected to controlled enzymatic degradation with alpha-amylase for 24 hours, 77 +/- 6% of the starch from the flight cotyledons was degraded compared to 58 +/- 12% in ground controls. The amylose content in starch was also higher in space-grown tissues. The good correlation between the amylose content and delta X/delta rho suggests, that the magnetic susceptibility of starch grains is related to their amylose content. Since the seedlings from the BRIC-03 experiment showed elevated post-flight ethylene levels, material from another flight experiment (GENEX) which had normal levels of ethylene was examined and showed no difference to ground controls in size distribution, density, delta X/delta rho and amylose content. Therefore the role of ethylene appears to be more important for changes in starch metabolism than microgravity.     

Updated Review of Planetary Atmospheric Electricity

This paper reviews the progress achieved in planetary atmospheric electricity, with focus on lightning observations by present operational spacecraft, aiming to fill the hiatus from the latest review published by Desch et al. (Rep. Prog. Phys. 65:955–997, 2002). The information is organized according to solid surface bodies (Earth, Venus, Mars and Titan) and gaseous planets (Jupiter, Saturn, Uranus and Neptune), and each section presents the latest results from space-based and ground-based observations as well as laboratory experiments. Finally, we review planned future space missions to Earth and other planets that will address some of the existing gaps in our knowledge.