The role of DNA repair on cell killing by charged particles.

It can be noted that it is not simple double strand breaks (dsb) but the non-reparable breaks that are associated with high biological effectiveness in the cell killing effect for high LET radiation. Here, we have examined the effectiveness of fast neutrons and low (initial energy = 12 MeV/u) or high (135 MeV/u) energy charged particles on cell death in 19 mammalian cell lines including radiosensitive mutants. Some of the radiosensitive lines were deficient in DNA dsb repair such as LX830, M10, V3, and L5178Y-S cells and showed lower values of relative biological effectiveness (RBE) for fast neutrons if compared with their parent cell lines. The other lines of human ataxia-telangiectasia fibroblasts, irs 1, irs 2, irs 3 and irs1SF cells, which were also radiosensitive but known as proficient in dsb repair, showed moderated RBEs. Dsb repair deficient mutants showed low RBE values for heavy ions. These experimental findings suggest that the DNA repair system does not play a major role against the attack of high linear energy transfer (LET) radiations. Therefore, we hypothesize that a main cause of cell death induced by high LET radiations is due to non-reparable dsb, which are produced at a higher rate compared to low LET radiations.     

Simulation experiments of the effect of space environment on bacteriophage and DNA thin films.

The main goal of PUR experiment (phage and uracil response) is to examine and quantify the effect of specific space conditions on nucleic acid models. To achieve this an improved method was elaborated for the preparation of DNA and bacteriophage thin films. The homogeneity of the films was controlled by UV spectroscopy and microscopy. To provide experimental evidence for the hypothesis that interplanetary transfer of the genetic material is possible, phage T7 and isolated T7 DNA thin films have been exposed to selected space conditions: intense UVC radiation (lambda=254 nm) and high vacuum (10(-4) Pa). The effects of DNA hydration, conformation and packing on UV radiation damage were examined. Characteristic changes in the absorption spectrum, in the electrophoretic pattern of DNA and the decrease of the amount of PCR products have been detected indicating the photodamage of isolated and intraphage DNA.     

Influence of long-term altered gravity on the swimming performance of developing cichlid fish: including results from the 2nd German Spacelab Mission D-2.

This study presents qualitative and quantitative data concerning gravity-dependent changes in the swimming behaviour of developing cichlid fish larvae (Oreochromis mossambicus) after a 9 resp. 10 days exposure to increased acceleration (centrifuge experiments), to reduced gravity (fast-rotating clinostat), changed accelerations (parabolic aircraft flights) and to near weightlessness (2nd German Spacelab Mission D-2). Changes of gravity initially cause disturbances of the swimming performance of the fish larvae. With prolonged stay in orbit a step by step normalisation of the swimming behaviour took place in the fish. After return to 1g earth conditions no somersaulting or looping could be detected concerning the fish, but still slow and disorientated movements as compared to controls occurred. The fish larvae adapted to earth gravity within 3-5 days. Fish seem to be in a distinct early developmental stages extreme sensitive and adaptable to altered gravity; However, elder fish either do not react or show compensatory behaviour e.g. escape reactions.     

Enzyme conversion of lignocellulosic plant materials for resource recovery in a Controlled Ecological Life Support System.

A large amount of inedible plant material composed primarily of the carbohydrate materials cellulose, hemicellulose, and lignin is generated as a result of plant growth in a Controlled Ecological Life-Support System (CELSS). Cellulose is a linear homopolymer of glucose, which when properly processed will yield glucose, a valuable sugar because it can be added directly to human diets. Hemicellulose is a heteropolymer of hexoses and pentoses that can be treated to give a sugar mixture that is potentially a valuable fermentable carbon source. Such fermentations yield desirable supplements to the edible products from hydroponically-grown plants such as rapeseed, soybean, cowpea, or rice. Lignin is a three-dimensionally branched aromatic polymer, composed of phenyl propane units, which is susceptible to bioconversion through the growth of the white rot fungus, Pluerotus ostreatus. Processing conditions, that include both a hot water pretreatment and fungal growth and that lead to the facile conversion of plant polysaccharides to glucose, are presented.     

James Webb Space Telescope: Project Overview

The James Webb Space Telescope (JWST) project at the NASA, Goddard Space Flight Center (GSFC) is responsible for the development, launch, flight, and science operations for the telescope. The project is in phase B with its launch scheduled for no earlier than June 2013. The project is a partnership among NASA, the European Space Agency (ESA), and the Canadian Space Agency (CSA). The JWST mission team is fully in place, including major ESA and CSA subcontractors. This provides an overview of the planned JWST science, current architecture focusing on the instrumentation, and mission status, including technology developments, and risks.     

Recent Breakthroughs in RF Photonics for Radar Systems

Radar systems require transmission of very high purity signals. Photonics is now mature enough to achieve analog transmission with very low noise, strong immunity, and wide-bandwidth even in harsh environments. We present our recent developments of optimized optical links dedicated to radar and multifunction systems     

HMS SCOTT ring laser gyro navigator integration

HMS SCOTT is a United Kingdom ocean survey vessel that hosts a state-of-the-art deep ocean mapping system which was designed, developed, and is currently maintained and periodically updated by the Space and Naval Warfare Systems Center, San Diego, (SSC San Diego). A recent update of this system consisted of the replacement of an obsolete and very costly to maintain inertial navigation system. Another reason for updating the ship's inertial system was to provide higher accuracy attitude data, than was previously available with the old inertial system, to the high resolution multi-beam sonar system in order to produce improved bathymetric charts. After conducting a market survey of suitable inertial navigator systems, the Kearfott SEANAV ring laser gyro navigator (RLGN) system, using a monolithic T-24 gyro, was selected to replace the old inertial system. The selection of the SEANAV RLGN was based on its relatively low cost, high reliability, and, particularly, the roll and pitch data accuracies of typically less than 1-arc-minute. This attitude data accuracy was key to enable a significant improvement in the bathymetry data accuracy. Two SEANAV systems were integrated with GPS receivers, a system master time code generator, and electro magnetic (EM) log and Doppler sonar speed sensors to provide the navigation portion of the mapping data. Operational testing of this updated system aboard HMS SCOTT, in November, 2003, showed a marked improvement in the quality of the map product due, in part, to the improved attitude data provided by the SEANAV RLGN system.     

Microdosimetric measurements of heavy ion tracks.

The biological effectiveness of radiations depends on the spatial pattern of ionizations and excitations produced by the charged particle tracks involved. Ionizations produced by both the primary ion and by energetic delta rays may contribute to the production of biologically relevant damage and to the concentration of damage which may effect the probability of repair. Although average energy concentration (dose) can be calculated using homogeneous track models, the energy is actually concentrated in small volumes containing segments of the ion and delta ray tracks. These local concentrations are studied experimentally using low pressure proportional counters, and theoretically, using Monte Carlo methods. Small volumes near an ion track may be traversed by a delta ray. If they are, the energy deposited will be similar to that produced by a single electron track in a low-energy x-ray irradiation. The probability of a delta ray interaction occurring decreases with the square of the radial distance from the track. The average energy deposited is the product of this probability and the energy deposited in an interaction. Average energy deposited calculated from measured interaction probability is in good agreement with the results of homogeneous track models.