Repair of radiation induced genetic damage under microgravity.

The influence of microgravity on the repair of radiation induced genetic damage in a temperature-conditional repair mutant of the yeast Saccharomyces cerevisiae (rad 54-3) was investigated onboard the IML-1 mission (January 22nd-30th 1992, STS-42). Cells were irradiated before the flight, incubated under microgravity at the permissive (22 degrees C) and restrictive (36 degrees C) temperature and afterwards tested for survival. The results suggest that repair may be reduced under microgravity.     

The stretched lens array (SLA) [spacecraft solar power]

At IECEC 2001, this team presented a paper on the new stretched lens array (SLA), including its evolution from the successful SCARLET array on the NASA/JPL Deep Space 1 spacecraft. Since that conference, the SLA team has made significant advances in SLA technology, including component-level improvements, array-level optimization, space environment exposure testing, and prototype hardware fabrication and evaluation. This paper describes the evolved version of the SLA, highlighting recent improvements in the lens, solar cell, photovoltaic receiver, rigid panel structure, and complete solar array wing. In addition to excellent durability in the space environment, the near-term SLA will provide outstanding wing-level performance parameters: 180 W/kg specific power; 300 W/m/sup 2/ power density; 300 V operational voltage; 85% savings in cell area (cm/sup 2//W) and cell-related cost ($/W) compared to planar arrays; 9 kW/m/sup 3/ stowed power at launch.     

Optical fiber wavelength division multiplexing

During flight, aircraft avionics transmit and receive RF signals to/from antennas over coaxial cables. As the density and complexity of onboard avionics increases, the electromagnetic interference (EMI) environment degrades proportionately, leading to decreasing signal-to-noise ratios (SNRs) and potential safety concerns. The coaxial cables are inherently lossy, limiting the RF signal bandwidth while adding considerable weight. To overcome these limitations, we have investigated a fiber optic communications link for aircraft that utilizes wavelength division multiplexing (WDM) to support the simultaneous transmission of multiple signals (including RF) over a single optical fiber. Optical fiber has many advantages over coaxial cable, particularly lower loss, greater bandwidth, and immunity to EMI. In this paper, we demonstrate that WDM can be successfully used to transmit multiple RF signals over a single optical fiber with no appreciable signal degradation. We investigate the transmission of FM and AM analog modulated signals, as well as FSK digital modulated signals, over a fiber optic link (FOL) employing WDM. We present measurements of power loss, delay, SNR, carrier-to-noise ratio (CNR), total harmonic distortion (THD), and bit error rate (BER). Our experimental results indicate that WDM is a fiber optic technology suitable for avionics applications.     

Three-dimensional transgenic cell model to quantify genotoxic effects of space environment.

In this paper we describe a three-dimensional, multicellular tissue-equivalent model, produced in NASA-designed, rotating wall bioreactors using mammalian cells engineered for genomic containment of multiple copies of defined target genes for genotoxic assessment. Rat 2 lambda fibroblasts, genetically engineered to contain high-density target genes for mutagenesis (Stratagene, Inc., Austin, TX), were cocultured with human epithelial cells on Cytodex beads in the High Aspect Ratio Bioreactor (Synthecon, Inc, Houston, TX). Multi-bead aggregates were formed by day 5 following the complete covering of the beads by fibroblasts. Cellular retraction occurred 8-14 days after coculture initiation culminating in spheroids retaining few or no beads. Analysis of the resulting tissue assemblies revealed: multicellular spheroids, fibroblasts synthesized collagen, and cell viability was retained for the 30-day test period after removal from the bioreactor. Quantification of mutation at the LacI gene in Rat 2 lambda fibroblasts in spheroids exposed to 0-2 Gy neon using the Big Blue color assay (Stratagene, Inc.), revealed a linear dose-response for mutation induction. Limited sequencing analysis of mutant clones from 0.25 or 1 Gy exposures revealed a higher frequency of deletions and multiple base sequencing changes with increasing dose. These results suggest that the three-dimensional, multicellular tissue assembly model produced in NASA bioreactors are applicable to a wide variety of studies involving the quantification and identification of genotoxicity including measurement of the inherent damage incurred in Space.     

Ground probing radar system

Two types of battery-powered, compact, ground probing radar (GPR) systems have been developed. The systems have CRT displays mounted on antenna units and are used to locate such underground objects as gas pipes, water pipes, and transmission cables. The performance of the systems and results of field tests are reported. The GPR detected 59 of 64 underground gas pipes; a 200-mm-diameter pipe is clearly detected at a depth of 2.3 m     

Swimming behaviour and calcium incorporation into inner ear otoliths of fish after vestibular nerve transection.

Previous investigations on neonate swordtail fish (Xiphophorus helleri) revealed that otolithic calcium incorporation (visualized using the calcium tracer alizarin complexone) and thus otolith growth had ceased after nerve transection, supporting a hypothesis according to which the gravity-dependent otolith growth is regulated neuronally. Subsequent investigations on larval cichlid fish (Oreochromis mossambicus) yielded contrasting results, repeatedly depending on the particular batch of cichlids investigated. Like most neonate swordtails, Type I cichlids revealed a stop of calcium incorporation after unilateral vestibular nerve transection. Their behaviour after transection was normal, and the otolithic calcium incorporation in controls of the same batch was symmetric. In Type II cichlids, however, vestibular nerve transection had no effect on otolithic calcium incorporation. They behaved kinetotically after transection (this kind of kinetosis was qualitatively similar to the swimming behaviour exhibited by larval cichlids during microgravity in the course of parabolic aircraft flights). The otolithic calcium incorporation in control animals was asymmetric. These results show that the effects of vestibular nerve transection as well as the efficacy of the mechanism, which regulates otolith growth/otolithic calcium incorporation, are--depending on the particular batch of animals--genetically predispositioned. In conclusion, the regulation of otolithic calcium incorporation is guided neuronally, in part via the vestibular nerve and, in part, via a further pathway, which remains to be addressed in the course of future investigations.     

Effect of hypergravity on carboanhydrase reactivity in inner ear ionocytes of developing cichlid fish.

It has been shown earlier that hypergravity slows down inner ear otolith growth in developing fish. Otolith growth in terms of mineralization mainly depends on the enzyme carboanhydrase (CA), which is responsible for the provision of the pH-value necessary for calcium carbonate deposition. Larval siblings of cichlid fish (Oreochromis mossambicus) were subjected to hypergravity (3 g, hg; 6 h) during development and separated into normally and kinetotically swimming individuals following the transfer to 1 g (i.e., stopping the centrifuge; kinetotically behaving fish performed spinning movements). Subsequently, CA was histochemically demonstrated in inner ear ionocytes (cells involved in the endolymphatic ion exchange) and enzyme reactivity was determined densitometrically. It was found that both the total macular CA-reactivity as well as the difference in reactivities between the left and the right maculae (asymmetry) were significantly lower (1) in experimental animals as compared to the 1 g controls and (2) in normally swimming hg-animals as compared to the kinetotically behaving hg-fish. The results are in complete agreement with earlier studies, according to which hypergravity induces a decrease of otolith growth and the otolithic calcium incorporation (visualized using the calcium-tracer alizarin complexone) of kinetotically swimming hg-fish was higher as compared to normally behaving hyper-g animals. The present study thus strongly supports the concept that a regulatory mechanism, which adjusts otolith size and asymmetry as well as otolithic calcium carbonate incorporation towards the gravity vector, acts via activation/deactivation of macular CA.     

Analog Computer Needed!

Science and technology advance neither steadily nor continuously. Concepts deemed obsolete and confined to oblivion find their niche in new developments. Discarded old methods revive as the key ingredients to new designs. Analog computing was the birthplace of two important technologies: Computer simulation, as applied to system design, was founded. The operational amplifier, to become the basis for modern linear circuitry, was perfected. Valuable as it was, analog simulation's quasi-hardware approach to analysis aggravated users as much as it aided them. When digital simulation languages arrived, simulation engineers readily adopted them. When the microprocessor transformed digital computers into inexpensive circuit components (more like gates, flip-flops, amplifiers, etc. than computers,) there arose unlimited prospects for digital control. But, the discrete digital and continuous analog worlds are not compatible. Timing discontinuities and variable resolution limitations create problems. Control system designs, especially microprocessor based ones, need laboratory development. For hands-on testing, the analog computer is as handy an instrument as a controls engineer could have. Its two unique and valuable functions are: Simulator of Systems to be Controlled... The electrical analogs or physical models, analog computer simulations offer predictable yet realistic representations of mechanisms and processes to be controlled. Programmable Linear Circuits Manifold... The terminal points for high quality, linear circuit devices, analog computer patch panels offer the only formal means of programing linear signal processing, interface and control circuits. This paper offers a discussion of these two analog computing uses.     

Minitact gyroscope-the low cost alternative

The allure of the solid state optical gyros is being challenged by the invention of a new spinning mass device, providing superior low noise drift performance, high reliability at a very low cost. The Minitact is a unique two axis gyroscope advancing the technology of spinning mass devices to set new low cost standards. Minitact provides all the low noise performance benefits of tuned rotor gyroscopes without the vagaries of fixture suspension systems. The Minitact design integrates together the advantages of a spherical hydrodynamic gas bearing and a three axis permanent magnet DC commutated, motor and torquer, into miniature two aids gyroscope with a dynamic range of greater than 10^-7. The design is described and performance data presented illustrating the capability of this low cost next generation rate gyroscope. The Minitact is the result of Integrated Product Development (IPD) and Design to Unit Production Cost (DTUPC) design processes and is expected to become the tactical performance benchmark     

A cost effective critical space systems design approach

NASA-ISC requires avionics platforms capable of serving a wide range of applications in a cost-effective manner. In part, making the avionics platform cost effective means adhering to open standards and supporting the integration of COTS products with custom products. Inherently, operation in space requires low power, mass, and volume while retaining high performance, reconfigurability, scalability, and upgradability. The Universal Mini-Controller (UMC) project is based on a modified PC/104-Plus architecture while maintaining full compatibility with standard COTS PC/104 products. The architecture consists of a library of stackable building block modules, which can be mixed and matched to meet a specific application. A set of NASA developed core building blocks, (e.g. processor card, analog input/output card, high level analog card, and a Mil-Std-1553 card) were constructed to meet critical functions and unique interfaces