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161.
Induction and repair of DNA strand breaks in bovine lens epithelial cells after high LET irradiation. 总被引:1,自引:0,他引:1
C Baumstark-Khan J Heilmann H Rink 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2003,31(6):1583-1591
The lens epithelium is the initiation site for the development of radiation induced cataracts. Radiation in the cortex and nucleus interacts with proteins, while in the epithelium, experimental results reveal mutagenic and cytotoxic effects. It is suggested that incorrectly repaired DNA damage may be lethal in terms of cellular reproduction and also may initiate the development of mutations or transformations in surviving cells. The occurrence of such genetically modified cells may lead to lens opacification. For a quantitative risk estimation for astronauts and space travelers it is necessary to know the relative biological effectiveness (RBE), because the spacial and temporal distribution of initial physical damage induced by cosmic radiation differ significantly from that of X-rays. RBEs for the induction of DNA strand breaks and the efficiency of repair of these breaks were measured in cultured diploid bovine lens epithelial cells exposed to different LET irradiation to either 300 kV X-rays or to heavy ions at the UNILAC accelerator at GSI. Accelerated ions from Z=8 (O) to Z=92 (U) were used. Strand breaks were measured by hydroxyapatite chromatography of alkaline unwound DNA (overall strand breaks). Results showed that DNA damage occurs as a function of dose, of kinetic energy and of LET. For particles having the same LET the severity of the DNA damage increases with dose. For a given particle dose, as the LET rises, the numbers of DNA strand breaks increase to a maximum and then reach a plateau or decrease. Repair kinetics depend on the fluence (irradiation dose). At any LET value, repair is much slower after heavy ion exposure than after X-irradiation. For ions with an LET of less than 10,000 keV micrometers-1 more than 90 percent of the strand breaks induced are repaired within 24 hours. At higher particle fluences, especially for low energetic particles with a very high local density of energy deposition within the particle track, a higher proportion of non-rejoined breaks is found, even after prolonged periods of incubation. At the highest LET value (16,300 keV micrometers-1) no significant repair is observed. These LET-dependencies are consistent with the current mechanistic model for radiation induced cataractogenesis which postulates that genomic damage to the surviving fraction of epithelial cells is responsible for lens opacification. 相似文献
162.
V Blum 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2003,31(7):1683-1691
The Closed Equilibrated Biological Aquatic System (C.E.B.A.S.) is an artificial aquatic ecosystem which contains teleost fishes, water snails, ammonia oxidizing bacteria and edible non-gravitropic water plants. It serves as a model for aquatic food production modules which are not seriously affected by microgravity and other space conditions. Its space flight version, the so-called C.E.B.A.S. MINI-MODULE was already successfully tested in the STS-89 and STS-90 (NEUROLAB) missions. It will be flown a third time in space with the STS-107 mission in January 2003. All results obtained so far in space indicate that the basic concept of the system is more than suitable to drive forward its development. The C.E.B.A.S. MINI-MODULE is located within a middeck locker with limited space for additional components. These technical limitations allow only some modifications which lead to a maximum experiment time span of 120 days which is not long enough for scientifically essential multi-generation-experiments. The first necessary step is the development of "harvesting devices" for the different organisms. In the limited space of the plant bioreactor a high biomass production leads to self-shadowing effects which results in an uncontrolled degradation and increased oxygen consumption by microorganisms which will endanger the fishes and snails. It was shown already that the latter reproduce excellently in space and that the reproductive functions of the fish species are not affected. Although the parent-offspring-cannibalism of the ovoviviparous fish species (Xiphophorus helleri) serves as a regulating factor in population dynamics an uncontrolled snail reproduction will also induce an increased oxygen consumption per se and a high ammonia concentration in the water. If harvesting locks can be handled by astronauts in, e. g., 4-week intervals their construction is not very difficult and basic technical solutions are already developed. The second problem is the feeding of the animals. Although C.E.B.A.S.-based aquaculture modules are designed to be closed food loop systems (edible herbivorous fish species and edible water plants) which are already verified on Earth this will not be possible in space without devices in which the animals are fed from a food storage. This has to be done at least once daily which would waste too much crew time when done by astronauts. So, the development of a reliable automated food dispenser has highest priority. Also in this case basic technical solutions are already elaborated. The paper gives a comprehensive overview of the proposed further C.E.B.A.S.-based development of longer-term duration aquatic food production modules. 相似文献
163.
D O Klymchuk E L Kordyum T V Vorobyova D K Chapman C S Brown 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2003,31(10):2283-2288
Changes in the vacuolation in root apex cells of soybean (Glycine max L. [Merr.]) seedlings grown in microgravity were investigated. Spaceflight and ground control seedlings were grown in the absence or presence of KMnO4 (to remove ethylene) for 6 days. After landing, in order to study of cell ultrastructure and subcellular free calcium ion distribution, seedling root apices were fixed in 2.5% (w/v) glutaraldehyde in 0.1 M cacodylate buffer and 2% (w/v) glutaraldehyde, 2.5% (w/v) formaldehyde, 2% (w/v) potassium antimonate K[Sb(OH)6] in 0.1 M K2HPO4 buffer with an osmolarity (calculated theoretically) of 0.45 and 1.26 osmol. The concentrations of ethylene in all spaceflight canisters were significantly higher than in the ground control canisters. Seedling growth was reduced in the spaceflight-exposed plants. Additionally, the spaceflight-exposed plants exhibited progressive vacuolation in the root apex cells, particularly in the columella cells, to a greater degree than the ground controls. Plasmolysis was observed in columella cells of spaceflight roots fixed in solutions with relatively high osmolarity (1.26 osmol). The appearance of plasmolysis permitted the evaluation of the water status of cells. The water potential of the spaceflight cells was higher than the surrounding fixative solution. A decrease in osmotic potential and/or an increase in turgor potential may have induced increases in cell water potential. However, the plasmolysed (i.e. non-turgid) cells implied that increases in water potential were accompanied with a decrease in osmotic potential. In such cells changes in vacuolation may have been involved to maintain turgor pressure or may have been a result of intensification of other vacuolar functions like digestion and storage. 相似文献
164.
K O'Brien H H Sauer 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2003,32(1):73-80
High-energy solar particles, produced in association with solar flares and coronal mass ejections, occasionally bombard the earth's atmosphere. resulting in radiation intensities additional to the background cosmic radiation. Access of these particles to the earth's vicinity during times of geomagnetic disturbances are not adequately described by using static geomagnetic field models. These solar fluxes are also often distributed non uniformly in space, so that fluxes measured by satellites obtained at great distances from the earth and which sample large volumes of space around the earth cannot be used to predict fluxes locally at the earth's surface. We present here a method which uses the ground-level neutron monitor counting rates as adjoint sources of the flux in the atmosphere immediately above them to obtain solar-particle effective dose rates as a function of position over the earth's surface. We have applied this approach to the large September 29-30, 1989 ground-level event (designated GLE 42) to obtain the magnitude and distribution of the solar-particle effective dose rate from an atypically large event. The results of these calculations clearly show the effect of the softer particle spectra associated with solar particle events, as compared with galactic cosmic rays, results in a greater sensitivity to the geomagnetic field, and, unlike cosmic rays, the near-absence of a "knee" near 60 degrees geomagnetic latitude. 相似文献
165.
分析了2000年和2001年期间52°N地区OI5577气辉强度的夜间变化特征和季节变化特征.利用由 OI5577气辉强度反演原子氧浓度峰值的方法反演出原子氧浓度的峰值,分析了峰值的夜间变化特征和季节变化特征.结果表明, OI5577气辉强度的夜间变化特征随季节变化, 2000年春季的夜间强度最大值出现在0000LT 之后,夏季和秋季的出现在0000LT之前,冬季的出现在0000LT,2001年春季和秋季的夜间强度最大值出现在0000LT之前,夏季和冬季的出现在0000LT;OI5577气辉强度在2000年2月份,8月份和10月份出现最大值,在2001年9月份有最大值.就主要特征而言,反演出的原子氧浓度峰值的夜间变化特征和季节变化特征分别与OI5577气辉强度的一致. 相似文献
166.
S. Seetha M.C. Ramadevi V.C. Babu M.R. Sharma N.S.R. Murthy B.N. Ashoka K.C. Shyama R. Kulkarni G. Meena P. Sreekumar 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2006,38(12):2995-2998
The Scanning Sky Monitor is one of the experiments onboard the ASTROSAT, an Indian multiwavelength astronomy satellite mission. This experiment will detect and monitor X-ray transients in the energy band 2–10 keV. It is similar in design to the ASM on RXTE. It consists of position-sensitive proportional counters with one-dimensional mask. We describe the configuration of the experiment. We also discuss some of the results obtained using a detector which has already been fabricated and tested in our laboratory. 相似文献
167.
Diane Sonya Wong Ryan Chornock Alexei V. Filippenko 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2006,38(12):2813-2815
We present results of optical follow-up observations of candidate ultra-luminous X-ray sources (ULXs). Using Keck optical spectroscopy, 17 of the candidates from the Colbert and Ptak [Colbert, E.J.M., Ptak, A.F. A catalog of candidate intermediate-luminosity X-ray objects. ApJS 143, 25–45, 2002] catalog have been identified; this is one of the largest sets of optical identifications of such objects thus far. Fifteen are background active galactic nuclei (AGN); two are foreground stars in our Galaxy. These findings are consistent with background and foreground object expectations, as derived from log N–log S relations. Also, the results are briefly discussed in terms of the spiral-galaxy/ULX connection. 相似文献
168.
H. Raichur B. Paul S. Naik N. Bhatt 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2006,38(12):2785-2787
High mass X-ray binary (HMXB) pulsars are of two types, persistent and transient. 4U1538−52 is a persistent HMXB whose orbit was previously measured to be circular but the RXTE observations revealed an eccentric orbit. We observed this system with RXTE-PCA in August 2003 and our timing analysis supports the eccentric orbit of the system. However, we do not find any evidence for orbital evolution.
Rotational and tidal interactions between the stars of a closed binary system result in apsidal motion which can be measured in systems with eccentric orbit. 4U0115+63 is a Be-transient HMXB whose eccentric orbit was well-determined during its 1978 outburst. We report preliminary results from analysis of data obtained during the 1999 outburst of this source with the RXTE-PCA. 相似文献
169.
T. Muoz-Darias I.G. Martínez-Pais J. Casares T.R. Marsh R. Cornelisse D. Steeghs V.S. Dhillon P.A. Charles 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2006,38(12):2762-2764
We present preliminary results of a simultaneous X-ray/optical campaign of the prototypical LMXB Sco X-1 at 1–10 Hz time resolution. Lightcurves of the high excitation Bowen/HeII emission lines and a red continuum at λc 6000 Å were obtained through narrow interference filters with ULTRACAM, and these were cross-correlated with simultaneous RXTE X-ray lightcurves. We find evidence for correlated variability, in particular when Sco X-1 enters the Flaring branch. The Bowen/HeII lightcurves lag the X-ray lightcurves with a light travel time which is consistent with reprocessing in the companion star while the continuum lightcurves have shorter delays consistent with reprocessing in the accretion disc. 相似文献
170.
V D Kern S Bhattacharya R N Bowman F M Donovan C Elland T F Fahlen B Girten M Kirven-Brooks K Lagel G B Meeker O Santos 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2001,27(5):1023-1030
During the construction phase of the International Space Station (ISS), early flight opportunities have been identified (including designated Utilization Flights, UF) on which early science experiments may be performed. The focus of NASA's and other agencies' biological studies on the early flight opportunities is cell and molecular biology; with UF-1 scheduled to fly in fall 2001, followed by flights 8A and UF-3. Specific hardware is being developed to verify design concepts, e.g., the Avian Development Facility for incubation of small eggs and the Biomass Production System for plant cultivation. Other hardware concepts will utilize those early research opportunities onboard the ISS, e.g., an Incubator for sample cultivation, the European Modular Cultivation System for research with small plant systems, an Insect Habitat for support of insect species. Following the first Utilization Flights, additional equipment will be transported to the ISS to expand research opportunities and capabilities, e.g., a Cell Culture Unit, the Advanced Animal Habitat for rodents, an Aquatic Facility to support small fish and aquatic specimens, a Plant Research Unit for plant cultivation, and a specialized Egg Incubator for developmental biology studies. Host systems (Figure 1A, B: see text), e.g., a 2.5 m Centrifuge Rotor (g-levels from 0.01-g to 2-g) for direct comparisons between g and selectable g levels, the Life Sciences Glovebox for contained manipulations, and Habitat Holding Racks (Figure 1B: see text) will provide electrical power, communication links, and cooling to the habitats. Habitats will provide food, water, light, air and waste management as well as humidity and temperature control for a variety of research organisms. Operators on Earth and the crew on the ISS will be able to send commands to the laboratory equipment to monitor and control the environmental and experimental parameters inside specific habitats. Common laboratory equipment such as microscopes, cryo freezers, radiation dosimeters, and mass measurement devices are also currently in design stages by NASA and the ISS international partners. 相似文献