Induction and repair of DNA strand breaks in bovine lens epithelial cells after high LET irradiation.

The lens epithelium is the initiation site for the development of radiation induced cataracts. Radiation in the cortex and nucleus interacts with proteins, while in the epithelium, experimental results reveal mutagenic and cytotoxic effects. It is suggested that incorrectly repaired DNA damage may be lethal in terms of cellular reproduction and also may initiate the development of mutations or transformations in surviving cells. The occurrence of such genetically modified cells may lead to lens opacification. For a quantitative risk estimation for astronauts and space travelers it is necessary to know the relative biological effectiveness (RBE), because the spacial and temporal distribution of initial physical damage induced by cosmic radiation differ significantly from that of X-rays. RBEs for the induction of DNA strand breaks and the efficiency of repair of these breaks were measured in cultured diploid bovine lens epithelial cells exposed to different LET irradiation to either 300 kV X-rays or to heavy ions at the UNILAC accelerator at GSI. Accelerated ions from Z=8 (O) to Z=92 (U) were used. Strand breaks were measured by hydroxyapatite chromatography of alkaline unwound DNA (overall strand breaks). Results showed that DNA damage occurs as a function of dose, of kinetic energy and of LET. For particles having the same LET the severity of the DNA damage increases with dose. For a given particle dose, as the LET rises, the numbers of DNA strand breaks increase to a maximum and then reach a plateau or decrease. Repair kinetics depend on the fluence (irradiation dose). At any LET value, repair is much slower after heavy ion exposure than after X-irradiation. For ions with an LET of less than 10,000 keV micrometers-1 more than 90 percent of the strand breaks induced are repaired within 24 hours. At higher particle fluences, especially for low energetic particles with a very high local density of energy deposition within the particle track, a higher proportion of non-rejoined breaks is found, even after prolonged periods of incubation. At the highest LET value (16,300 keV micrometers-1) no significant repair is observed. These LET-dependencies are consistent with the current mechanistic model for radiation induced cataractogenesis which postulates that genomic damage to the surviving fraction of epithelial cells is responsible for lens opacification.     

ARTEMIS Mission Design

The ARTEMIS mission takes two of the five THEMIS spacecraft beyond their prime mission objectives and reuses them to study the Moon and the lunar space environment. Although the spacecraft and fuel resources were tailored to space observations from Earth orbit, sufficient fuel margins, spacecraft capability, and operational flexibility were present that with a circuitous, ballistic, constrained-thrust trajectory, new scientific information could be gleaned from the instruments near the Moon and in lunar orbit. We discuss the challenges of ARTEMIS trajectory design and describe its current implementation to address both heliophysics and planetary science objectives. In particular, we explain the challenges imposed by the constraints of the orbiting hardware and describe the trajectory solutions found in prolonged ballistic flight paths that include multiple lunar approaches, lunar flybys, low-energy trajectory segments, lunar Lissajous orbits, and low-lunar-periapse orbits. We conclude with a discussion of the risks that we took to enable the development and implementation of ARTEMIS.     

Far ultraviolet imaging from the IMAGE spacecraft. 2. Wideband FUV imaging

The Far Ultraviolet Wideband Imaging Camera (WIC) complements the magnetospheric images taken by the IMAGE satellite instruments with simultaneous global maps of the terrestrial aurora. Thus, a primary requirement of WIC is to image the total intensity of the aurora in wavelength regions most representative of the auroral source and least contaminated by dayglow, have sufficient field of view to cover the entire polar region from spacecraft apogee and have resolution that is sufficient to resolve auroras on a scale of 1 to 2 latitude degrees. The instrument is sensitive in the spectral region from 140–190 nm. The WIC is mounted on the rotating IMAGE spacecraft viewing radially outward and has a field of view of 17° in the direction parallel to the spacecraft spin axis. Its field of view is 30° in the direction perpendicular to the spin axis, although only a 17°×17° image of the Earth is recorded. The optics was an all-reflective, inverted Cassegrain Burch camera using concentric optics with a small convex primary and a large concave secondary mirror. The mirrors were coated by a special multi-layer coating, which has low reflectivity in the visible and near UV region. The detector consists of a MCP-intensified CCD. The MCP is curved to accommodate the focal surface of the concentric optics. The phosphor of the image intensifier is deposited on a concave fiberoptic window, which is then coupled to the CCD with a fiberoptic taper. The camera head operates in a fast frame transfer mode with the CCD being read approximately 30 full frames (512×256 pixel) per second with an exposure time of 0.033 s. The image motion due to the satellite spin is minimal during such a short exposure. Each image is electronically distortion corrected using the look up table scheme. An offset is added to each memory address that is proportional to the image shift due to satellite rotation, and the charge signal is digitally summed in memory. On orbit, approximately 300 frames will be added to produce one WIC image in memory. The advantage of the electronic motion compensation and distortion correction is that it is extremely flexible, permitting several kinds of corrections including motions parallel and perpendicular to the predicted axis of rotation. The instrument was calibrated by applying ultraviolet light through a vacuum monochromator and measuring the absolute responsivity of the instrument. To obtain the data for the distortion look up table, the camera was turned through various angles and the input angles corresponding to a pixel matrix were recorded. It was found that the spectral response peaked at 150 nm and fell off in either direction. The equivalent aperture of the camera, including mirror reflectivities and effective photocathode quantum efficiency, is about 0.04 cm². Thus, a 100 Rayleigh aurora is expected to produce 23 equivalent counts per pixel per 10 s exposure at the peak of instrument response.     

Some comparisons of orbit determination and parameter estimation based on LAGEOS 1 and LAGEOS 2 data

The modelling accuracy of the LAGEOS 1 orbit was continously improved since its launch in 1976. In spite of these experiences the modelling accuracy of LAGEOS 2 is still about 20 per cent worse. Considering e. g. only the influence of different gravity field models it has been shown that the orbital fits for arc lengths of one month is generally about 25 mm for LAGEOS 1 and more than 30 mm for LAGEOS 2. This is mainly due to the fact that LAGEOS 2 has not yet been used for the determination of most gravity field models. The influences of different model parameters on the estimation of station coordinates, Earth rotation parameters, the geogravitational coefficient, the radiation pressure coefficient, and the empirical acceleration has been studied. The differences and peculiarities of both satellites are discussed. Although the analysis of LAGEOS 2 data still does not reach the high level of LAGEOS 1 combination solutions using both satellites allow new insights, higher accuracies, and a higher time resolution for the parameters and phenomena investigated.     

Radar Doppler Spectra of Turbulent Ionized Wakes

The evaluation of the double convolution integrat involved in the expression of the radar response to scattering from a turbulent ionized wake is simplified by the approximation technique presented here, so that the Doppler spectrum parameters can be explicitly expressed in terms of the wake characteristics.