Life sciences flight hardware development for the International Space Station.

During the construction phase of the International Space Station (ISS), early flight opportunities have been identified (including designated Utilization Flights, UF) on which early science experiments may be performed. The focus of NASA's and other agencies' biological studies on the early flight opportunities is cell and molecular biology; with UF-1 scheduled to fly in fall 2001, followed by flights 8A and UF-3. Specific hardware is being developed to verify design concepts, e.g., the Avian Development Facility for incubation of small eggs and the Biomass Production System for plant cultivation. Other hardware concepts will utilize those early research opportunities onboard the ISS, e.g., an Incubator for sample cultivation, the European Modular Cultivation System for research with small plant systems, an Insect Habitat for support of insect species. Following the first Utilization Flights, additional equipment will be transported to the ISS to expand research opportunities and capabilities, e.g., a Cell Culture Unit, the Advanced Animal Habitat for rodents, an Aquatic Facility to support small fish and aquatic specimens, a Plant Research Unit for plant cultivation, and a specialized Egg Incubator for developmental biology studies. Host systems (Figure 1A, B: see text), e.g., a 2.5 m Centrifuge Rotor (g-levels from 0.01-g to 2-g) for direct comparisons between g and selectable g levels, the Life Sciences Glovebox for contained manipulations, and Habitat Holding Racks (Figure 1B: see text) will provide electrical power, communication links, and cooling to the habitats. Habitats will provide food, water, light, air and waste management as well as humidity and temperature control for a variety of research organisms. Operators on Earth and the crew on the ISS will be able to send commands to the laboratory equipment to monitor and control the environmental and experimental parameters inside specific habitats. Common laboratory equipment such as microscopes, cryo freezers, radiation dosimeters, and mass measurement devices are also currently in design stages by NASA and the ISS international partners.     

Molecular alterations in tumorigenic human bronchial and breast epithelial cells induced by high LET radiation.

Carcinogenesis is a multi-stage process with sequence of genetic events governing the phenotypic expression of a series of transformation steps leading to the development of metastatic cancer. In the present study, immortalized human bronchial (BEP2D) and breast (MCF-10F) cells were irradiated with graded doses of either 150 keV/micrometer alpha particles or 1 GeV/nucleon 56Fe ions. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Cell fusion studies indicated that radiation-induced tumorigenic phenotype in BEP2D cells could be completely suppressed by fusion with non-tumorigenic BEP2D cells. The differential expressions of known genes between tumorigenic bronchial and breast cells induced by alpha particles and their respective control cultures were compared using cDNA expression array. Among the 11 genes identified to be differentially expressed in BEP2D cells, three (DCC, DNA-PK and p21(CIP1)) were shown to be consistently down-regulated by 2 to 4 fold in all the 5 tumor cell lines examined. In contrast, their expressions in the fusion cell lines were comparable to control BEP2D cells. Similarly, expression levels of a series of genes were found to be altered in a step-wise manner among tumorigenic MCF-10F cells. The results are highly suggestive that functional alterations of these genes may be causally related to the carcinogenic process.     

Development of thermal sensors and drilling systems for lunar and planetary regoliths

We consider some novel concepts for thermal properties experiments aboard lunar landers or rovers, that may lead to an improved understanding of both the structure of the lunar near surface layers and the lunar thermal history. The new instruments could be developed using the experience and heritage from recently developed systems, like the Rosetta Lander thermal conductivity experiment MUPUS and existing designs used for terrestrial measurements of thermal conductivity. We describe shortly the working principle of such sensors and the main challenges faced when using them in the airless regolith layers of the Moon or other airless bodies. In addition new concepts to create appropriate drill holes for thermal and other measurements in the lunar regolith are discussed.     

Lower-mesospheric inversion layers over brazilian equatorial region using TIMED/SABER temperature profiles

Lower-mesospheric inversion layers (MILs) were studied using the temperature profiles observed by TIMED/SABER over Cariri (7.5°S, 36.5°W), Brazil, in 2005. A total 175 MILs were identified with the maximum occurrence in April and October and the minimum in January and July. The lower MIL is located in a height region from 70 to 90 km, with the peak at around 83 ± 4 km with the temperature of 205 ± 5 K, and the thickness of 4–10 km. The results show large amplitudes of MILs during equinoxes and minimum in solstices, with a clear semiannual variation. A general feature of lower MIL in monthly mean profile was observed twice a year, one from February to May, and the other from August to October with a downward shift of the top level. These results suggest that formation and long persistence of MIL is an important factor to investigate propagation of atmospheric gravity waves in the mesosphere-lower thermosphere (MLT) region.     

Remote upgrading of a space-borne instrument

The main purposes of experiment “Obstanovka” (“Environment” in Russian) consisting of several instruments are to measure a set of electromagnetic and plasma phenomena characterizing the space weather conditions, and to evaluate how such a big and highly energy consuming body as the International Space Station disturbs the surrounding plasma, and how the station itself is charged due to the operation of so many instruments, solar batteries, life supporting devices, etc. Two identical Langmuir electrostatic probes are included in the experiment “Obstanovka”. In this paper the Langmuir probes for “Obstanovka” experiment are described, including the choice of geometry (spherical or cylindrical), a more reliable method for the sweep voltage generation, an adaptive algorithm for the probe’s operation. Special attention is paid to the possibility for remote upgrading of the instrument from the ground using the standard communication channels.     

Using gamma-ray burst prompt emission to probe relativistic shock acceleration

It is widely accepted that the prompt transient signal in the 10 keV–10 GeV band from gamma-ray bursts (GRBs) arises from multiple shocks internal to the ultra-relativistic expansion. The detailed understanding of the dissipation and accompanying acceleration at these shocks is a currently topical subject. This paper explores the relationship between GRB prompt emission spectra and the electron (or ion) acceleration properties at the relativistic shocks that pertain to GRB models. The focus is on the array of possible high-energy power-law indices in accelerated populations, highlighting how spectra above 1 MeV can probe the field obliquity in GRB internal shocks, and the character of hydromagnetic turbulence in their environs. It is emphasized that diffusive shock acceleration theory generates no canonical spectrum at relativistic MHD discontinuities. This diversity is commensurate with the significant range of spectral indices discerned in prompt burst emission. Such system diagnostics are now being enhanced by the broad-band spectral coverage of bursts by the Fermi Gamma-Ray Space Telescope; while the Gamma-Ray Burst Monitor (GBM) provides key diagnostics on the lower energy portions of the particle population, the focus here is on constraints in the non-thermal, power-law regime of the particle distribution that are provided by the Large Area Telescope (LAT).     

Study of GNSS-measured ionospheric total electron content variations generated by powerful HF-heating

The purpose of this work is to report the experimental evidences for the influence of perturbations in the electron density in the dayside mid-latitude ionosphere, that are caused by high-frequency heating of the F2 layer, on the GNSS signals. The experiments were carried out at the Sura heater (Radio Physical Research Institute, N. Novgorod). During the sessions of ionospheric heating with different time modulations of the radiated power the rays linking the navigational satellites with the ground receiver intersected the heated region. Variations in the total electron content (TEC) were studied; these variations are proportional to the reduced phases of navigational signals. It is shown that with the square-wave modulation of the radiated power (with periods of 1, 6, 10 and 15 min), perturbations with periods of the main modulation of heating and its harmonics appear in the spectrum of TEC variations. Examples are presented of identification of the heating-induced variations in TEC, including determination of the amplitudes and time characteristics of these variations.     

The origin of the prompt optical emission in GRB 060111B

The detection of a bright optical emission measured with good temporal resolution during the prompt phase makes GRB 060111B a rare event that is especially useful for constraining theories of the prompt optical emission. Comparing this burst with other GRBs with evidence of optical peaks, we find that the optical peak epoch (t_p) is anti-correlated with the high energy burst energetic assuming an isotropic energy release (E_iso) in agreement with Liang et al. (2009), and that the steeper is the post-peak afterglow decay, the less is the agreement with the correlation. GRB 060111B is among the latters and it does not match the correlation. The Cannonball scenario is also discussed and we find that this model cannot be excluded for GRB 060111B.     

Gamma ray bursts: Short vs. long

Short and long GRBs are thought to be two distinct classes based on their different duration and spectrum. Through the spectral analysis of two similarly selected samples of BATSE short and long GRBs, we show that short GRBs are harder than long events, confirming what found from the comparison of their hardness ratio. However, this spectral diversity seems to be due to a harder low energy spectral component of short GRBs, rather than a (slightly higher) peak energy. Interestingly short GRBs have a spectrum which is similar to the spectrum of the emission of the first 1–2 s of long events. We find evidence that short GRBs are inconsistent with the E_peak–E_iso correlation defined by long bursts while they follow the same E_peak–L_iso correlation of long GRBs. These results, coupled to the similar variability timescale of short events and the first seconds of long ones, suggest that a common (or similar) dissipation mechanism could operate in both classes. The difference in the duration would then be due mainly to the central engine lifetime.