The effects of composting on the nutritional composition of fibrous bio-regenerative life support systems (BLSS) plant waste residues and its impact on the growth of Nile tilapia (Oreochromis niloticus)

Utilization of bio-regenerative life support systems (BLSS) plant waste residues as a nutritional source by Nile tilapia (Oreochromis niloticus) has proven problematic as a result of high concentrations of fibrous compounds in the plant waste residues. Nutritional improvement of plant waste residues by composting with the oyster mushroom (Pleurotus ostreatus), and the effects on growth and nutrient utilization of Nile tilapia fed such residues were evaluated. Five Nile tilapia (mean weight = 70.9 ± 3.1 g) were stocked in triplicate aquaria and fed one of two experimental diets, cowpea (CP) and composted cowpea (CCP), twice daily for a period of 8 weeks. Composting of cowpea residue resulted in reduced concentrations of nitrogen-free extract, hemi-cellulose and trypsin inhibitor activity, though trypsin inhibitor activity remained high. Composting did not reduce crude fiber, lignin, or cellulose concentrations in the diet. No significant differences (P < 0.05) were observed in weight gain, specific growth rate, survival rate, daily consumption, and food conversion ratio between tilapia fed CP and CCP. These results suggest that P. ostreatus is not a suitable candidate for culture in conjunction with the culture of Nile tilapia. Additional work is needed to determine what, if any, benefit can be obtained from incorporating composted residue as feed for Nile tilapia.     

Optimization of moisture content for wheat seedling germination in a cellulose acetate medium for a space flight experiment.

The Porous Tube Plant Nutrient Delivery System (PTPNDS), a hydrophilic, microporous ceramic tube hydroponic system designed for microgravity, will be tested in a middeck locker of the Space Shuttle. The flight experiment will focus on hardware operation and assess its ability to support seed germination and early seedling growth in microgravity. The water controlling system of the PTPNDS hardware has been successfully tested during the parabolic flight of the KC-135. One challenge to the development of the space flight experiment was to devise a method of holding seeds to the cylindrical porous tube. The seed-holder must provide water and air to the seed, absorb water from the porous tube, withstand sterilization, provide a clear path for shoots and roots to emerge, and be composed of flight qualified materials. In preparation for the flight experiment, a wheat seed-holder has been designed that utilizes a cellulose acetate plug to facilitate imbibition and to hold the wheat seeds in contact with the porous tube in the correct orientation during the vibration of launch and the microgravity environment of orbit. Germination and growth studies with wheat at a range of temperatures showed that optimal moisture was 78% (by weight) in the cellulose acetate seed holders. These and other design considerations are discussed.     

Organizing and managing satellite solar power

Building an organization and management structure to create, launch, utilize and protect a satellite solar power energy system will require a global policy for the beneficial use of SSP. The fundamental organizational tasks are: (1) R&D, achieved through a project organization characterized by the integrated management of applied science, development research and construction engineering; (2) investment, generated by a series of groups creating financial vehicles for public and private investment; (3) transmission and distribution, characterized by attention to an engineering and maintenance process emphasizing high reliability; and (4) crisis response, demanding readiness for instant response to potential internal or external scenarios. A differentiated global organization spanning the long timeframe of SSP will need to have a central management core representative of all parts of the organization, with the capacity for self-renewal and re-adaptation. To be successful over its long timeframe, the SSP organization will need to build continuity and public confidence through intergenerational communication, public education, and community outreach. Integrating structures must be created at all levels of the organization, and should encompass joint work tasks and information-sharing among both industrial and government members. Developmental and alliance partners who support the formation and financing of a differentiated satellite solar power organization will share commensurately in the technologies and competencies that are created.     

Composition and physical properties of starch in microgravity-grown plants.

The effect of spaceflight on starch development in soybean (Glycine max L., BRIC-03) and potato (Solanum tuberosum, Astroculture-05) was compared with ground controls by biophysical and biochemical measurements. Starch grains from plants from both flights were on average 20-50% smaller in diameter than ground controls. The ratio delta X/delta rho (delta X --difference of magnetic susceptibilities, delta rho--difference of densities between starch and water) of starch grains was ca. 15% and 4% higher for space-grown soybean cotyledons and potato tubers, respectively, than in corresponding ground controls. Since the densities of particles were similar for all samples (1.36 to 1.38 g/cm3), the observed difference in delta X/delta rho was due to different magnetic susceptibilities and indicates modified composition of starch grains. In starch preparations from soybean cotyledons (BRIC-03) subjected to controlled enzymatic degradation with alpha-amylase for 24 hours, 77 +/- 6% of the starch from the flight cotyledons was degraded compared to 58 +/- 12% in ground controls. The amylose content in starch was also higher in space-grown tissues. The good correlation between the amylose content and delta X/delta rho suggests, that the magnetic susceptibility of starch grains is related to their amylose content. Since the seedlings from the BRIC-03 experiment showed elevated post-flight ethylene levels, material from another flight experiment (GENEX) which had normal levels of ethylene was examined and showed no difference to ground controls in size distribution, density, delta X/delta rho and amylose content. Therefore the role of ethylene appears to be more important for changes in starch metabolism than microgravity.     

Soybean cotyledon starch metabolism is sensitive to altered gravity conditions.

We have demonstrated that etiolated soybean seedlings grown under the altered gravity conditions of clinorotation (1 rpm) and centrifugation (5xg) exhibit changes in starch metabolism. Cotyledon starch concentration was lower (-28%) in clinorotated plants and higher (+24%) in centrifuged plants than in vertical control plants. The activity of ADP-glucose pyrophosphorylase in the cotyledons was affected in a similar way, i.e. lower (-37%) in the clinorotated plants and higher (+22%) in the centrifuged plants. Other starch metabolic enzyme activities, starch synthase, starch phosphorylase and total hydrolase were not affected by the altered gravity treatments. We conclude that the observed changes in starch concentrations were primarily due to gravity-mediated differences in ADP-glucose pyrophosphorylase activity.     

Calibration techniques for magnetometers implementing on-board de-spinning algorithms

The Fluxgate Magnetometer experiments on-board the European Space Agency’s four spacecraft Cluster Mission have the capability to store sampled magnetic field vectors in the instrument memory, either as a full resolution ‘event capture’ or as spin-resolution vectors transformed into a non-spinning co-ordinate system (de-spun). The latter capability has ensured a dataset is available which extends the partial orbital coverage achieved during nominal operations in the first years of operation. The on-board de-spin is achieved using a Walsh function with Haar coefficients and allows for up to 27 h additional data per non-coverage interval. A number of commissioning orbits were used to verify the accuracy of data collected by the de-spin mode, whereby individual spacecraft were operated separately in a number of standard normal sampling and de-spin mode combinations. Up to the present time, this data has not been available to the Cluster community. We present results here comparing the performance of the on-board de-spin algorithm versus the normal sampling modes over a number of boundary layer crossings, describe the techniques used for calibration and timeline recovery, and outline the context in which the data may be usable in future studies.     

On the effects of electron-cyclotron masers during flares

First recognized by Wu and Lee (Ap. J. 230, 621, 1979), electron-cyclotron masers can be activated under very mild conditions. Large growth rates can occur even for relatively mild anisotropies in the electron velocity distribution, e.g., the one-sided loss cones that commonly occur when electrons with small pitch angles precipitate into high density regions at the footpoints of flaring loops while others are reflected in the converging field in the corona. Maser action can plausibly occur at the second harmonic of the local gyrofrequency and so explain certain very bright (? 10¹⁰ K) microwave bursts from the sun and other stars. However, the preponderance of the energy is at the first harmonic.We suggest that masers operating at the local gyrofrequency in a flaring loop generate radiation at decimeter wavelengths that is a significant fraction of the total energy of the flare, in fact (and not coincidentally) comparable with the energy in electrons associated with hard X-ray bursts. Essentially all of the radio energy is trapped in the corona and serves to produce localized heating in a volume large compared with the energy release region. Thus it can transfer energy by radiation from one magnetic loop to another, possibly inducing further instabilities, and spreading the course of the flare. Eventually the energy probably escapes the corona as soft X-rays. The electron-cyclotron maser saturates by extracting the perpendicular energy of the electrons, thereby diffusing them into the loss cone at the maximum possible rate; the enhanced precipitation into the footpoints can produce bright emission in hard X-rays, EUV and Hα and remove any necessity for directive acceleration in the energy release region.Details of the proposed mechanism and effects are contained in two papers by Melrose and Dulk (Ap. J. 259, 1982).This work was sponsored by NASA under grants NAGW-91 and NSG-7287 to the University of Colorado.