Reduced lymphocyte activation in space: role of cell-substratum interactions.

We investigated the effect of substratum adhesiveness on stimulated lymphocyte blastogenesis by reducing and blocking cell adhesion with poly (2-hydroxyethyl methacrylate) (poly-HEMA) in a simple on-ground system. Cells grown on medium-thick and thick poly-HEMA films were rounded in shape and displayed no signs of spreading. By contrast, on tissue culture plastic and very thin poly-HEMA films, they showed clear signs of spreading. The mitogenic response of lymphocytes grown on thick poly-HEMA films was reduced by up to 68% of the control (tissue culture plastic). Interferon-gamma production was near zero when the cells were grown on the least adhesive substratum. On uncoated plastic, activated lymphocytes subjected to high gravity (20g) exhibited an increased proliferation rate (40%) compared with 1g. By contrast, on poly-HEMA, high gravity did not improve lymphocyte responsiveness. These results show that activated lymphocytes need to anchor and spread prior to achieving an optimal proliferation response. We conclude that decreased lymphocyte adhesion could contribute to the depressed in vitro lymphocyte responsiveness found in the microgravity conditions of space flight.     

The Freja wave and plasma density experiment

The Wave Experiment, F4, on the Swedish/German satelliteFreja, is designed to measure the electric wave fields up to 4 MHz, the magnetic wave fields up to 16 kHz and the plasma density and its relative variations up to 2 kHz. Six wave signals and four density probe signals can be measured simultaneously. The wave forms of all signals are transmitted to ground without any analysis onboard. The limited TM allocation does not allow continuous sampling of the wave signals, so normally the measurements are made in snapshots of various lengths dependent on sampling frequency, etc. Continuous sampling can be made for shorter time periods by using a 6 Mbyte memory as a buffer.     

Pioneer 10 Doppler data analysis: Disentangling periodic and secular anomalies

This paper reports the results of an analysis of the Doppler tracking data of Pioneer probes which did show an anomalous behaviour. A software has been developed for the sake of performing a data analysis as independent as possible from that of Anderson et al. [Anderson, J., Laing, P.A., Lau, E.L., Liu, A.S., Nieto, M.M., Turyshev, S.G. Study of the anomalous acceleration of Pioneer 10 and 11. Phys. Rev. D 65, 082004, 2002], using the same data set. A first output of this new analysis is a confirmation of the existence of a secular anomaly with an amplitude about 0.8 nm s⁻² compatible with that reported by Anderson et al. A second output is the study of periodic variations of the anomaly, which we characterize as functions of the azimuthal angle φ$\phi$ defined by the directions Sun–Earth Antenna and Sun-Pioneer. An improved fit is obtained with periodic variations written as the sum of a secular acceleration and two sinusoids of the angles φ$\phi$ and 2φ$2\phi$. The tests which have been performed for assessing the robustness of these results are presented.     

Integrated Planning and Execution for Autonomous Spacecraft

An autonomous spacecraft must balance long-term and short-term considerations. It must perform purposeful activities that ensure long-term science and engineering goals are achieved and ensure that it maintains positive resource margins. This requires planning in advance to avoid a series of shortsighted decisions that can lead to failure. However, it must also respond in a timely fashion to a somewhat dynamic and unpredictable environment. Thus, in terms of high-level, goal-oriented activity, spacecraft plans must often be modified due to fortuitous events such as early completion of observations and setbacks such as failure to acquire a guidestar for a science observation. This describes an integrated planning and execution architecture that supports continuous modification and updating of a current working plan in light of a changing operating context.