Inactivation, mutation induction and repair in Bacillus subtilis spores irradiated with heavy ions.

Studies on the response of bacterial spores to accelerated heavy ions (HZE particles) help in understanding problems of space radiobiology and exobiology. Layers of spores of Bacillus subtilis strains, differing in repair capabilities, were irradiated with accelerated boron, carbon and neon ions of linear energy transfer (LET) values up to 14000 MeV cm2/g. Inactivation as measured by loss of colony forming ability and induction of mutations as measured by reversion to histidine prototrophy and resistance to 150 micrograms/ml sodium azide were tested, as well as the influence of repair processes on these effects. For inactivation, the cross-sectional values sigma plotted as a function of LET follow a saturation curve. The plateau, which is reached around a LET of 2000 MeV cm2/g, occurs at 2.5 x 10(-9) cm2, a value in good agreement with the dimensions of the spore protoplast. Lethal damage produced at LET values < 2000 MeV cm2/g is reparable. Recombination repair is more effective than excision repair. At higher LET values, lethal damage could not be reconstituted by the repair mechanisms studied. In addition, at these high LET values, the frequency of induced mutations was drastically decreased. The data support the assumption of at least two qualitatively different types of lesion, depending on the LET of the affecting heavy ion.     

Solar and stellar seismology

This article reviews solar and stellar seismology, with emphasis on the enormous progress which has been made recently in the observation and understanding of solar p-modes. Precision measurements of p-mode frequencies and frequency splittings allow a greater understanding of the structure of the solar interior, while p-mode amplitudes and linewidths shed light on the mode excitation mechanism, which is probably stochastic excitation by turbulent convection. The prospects for making similar measurements on other stars are also discussed.     

Recursive Computation of the Generalized Q Function

A recursive method of computing values of the generalized Q function is described. The Q function is also interpreted in terms of noncentral chi-square distributed random variables, and in terms of the difference between Poisson random variables.     

Radio physics of the outer solar system

The remote sensing of low frequency nonthermal radio emission is the astronomy of field and particle phenomena. Observations conducted from space lead to information about the composition and dynamic processes occurring in planetary magnetospheres as well as within the interplanetary and interstellar medium. The potential of this technique is demonstrated by considering observations obtained from Earth orbit missions.This is one of the publications by the Science Advisory Group.     

Elemental and isotopic abundances of the volatile elements in the outer planets

Certain fundamental scientific problems of a cosmological as well as cosmogonic character, may be solved by the insertion of entry probes into the atmospheres of the outer planets. It is recommended that attempts be made to determine the elemental and isotopic abundances of H, D, He³, He⁴, C, N, O, S, and the rare gas elements. These determinations should cast much light on the processes which participated in the assembly of the giant planets. This would give powerful boundary conditions on theories of the origin of the solar system, and would also give additional experimental information bearing on cosmology.This is one of the publications by the Science Advisory Group.     

Italy's contribution, from a medical standpoint, to the space safety of payload scientists, and perspectives for the future

In Italy, the selection of the Italian payload scientists has been performed according to the Spacelab Program of ESA. Twenty-four subjects underwent a screening performed by the Health Service of Italian Air Force. They were requested to pass an exercise test on treadmill and another ten-minute test on centrifuge, subject to the effect of +3 Gz. The authors briefly describe the results of the test. Noteworthy is the determination of Central Flicker Fusion Frequency. This parameter makes it possible to assess the endurance level of the subject, much earlier than other techniques (e.g. EKG). The importance of an accurate preliminary screening is emphasized as well as of successive training periods. Future studies will be undertaken to compare evoked cortical potentials with behaviour parameters of space safety, with a view to setting up a subtle tool of evaluation for both future candidates and payload scientists.     

X-ray scattering by intergalactic dust

The imaging capabilities of the Exosat and Einstein satellites at soft X-ray wavelengths have begun to show that suitable Galactic X-ray sources have extended ( 10 arcmin) haloes due to scattering of soft X-rays by interstellar dust. A simple argument suggests that similar haloes, due to scattering by intergalactic dust, should exist around distant (z 1) quasars and detailed analysis confirms this conclusion. A search for such haloes around suitable X-ray quasars could provide valuable, model-independent, constraints on the amount and origin of intergalactic dust.     

Biological studies of martian soil analogues

Results of the study of the influence of Martian soil analogues, both as described by American scientists and as prepared by us, and of hydrogen peroxide on the viability of microorganisms are presented. The experiments were carried out using mixtures of soil analogues with desert soil and black earth (chernozem) samples, and pure cultures of microorganism. Microorganisms capable of withstanding a concentration of hydrogen peroxide in the medium as high as 1.5–2.0% were isolated. None of the 40 strains of microorganisms studied, all belonging to different systematic and physiological groups, exhibited growth inhibition on solid media in the presence of Martian soil analogues. In view of the fact that Martian soil cannot contain microorganisms in great quantities, we suggest using electroadsorption for their concentration, to make detection reliable. A device was designed for this purpose, using the principle of electroadsorption on a polarisable carrier (sterile cotton wool or cheesecloth). The concentrated suspension of microorganisms thus obtained was then characterized by various physicochemical methods.     

Experiments with injection of powerful plasma jet into the ionosphere

This paper describes two rocket experiments “Aelita” with high power lithium plasma injection. The results of onboard magnetometer, massspectrometer, photometer, plasma, corpuscular and ground radar measurements are given. Dynamics and structure of plasma formation are discussed.