Microbial stowaways: inimitable survivors or hopeless pioneers?

Abstract The resiliency of prokaryotic life has provided colonization across the globe and in the recesses of Earth's most extreme environments. Horizontal gene transfer provides access to a global bank of genetic resources that creates diversity and allows real-time adaptive potential to the clonal prokaryotic world. We assess the likelihood that this Earth-based strategy could provide survival and adaptive potential, in the case of microbial stowaways off Earth. Key Words: Bacillus-Horizontal gene transfer-Bacteria-Earth Mars-Evolution. Astrobiology 12, 710-715.     

径向导叶内流动分离特性的试验研究和控制

采用接触式探针和粒子图象测速技术(PIV)对导叶式离心泵径向导叶内流场的流动特性进行了研究。基于圆柱形微型三孔探针测试结果对5种流量下导叶的压力恢复能力进行了评估,测孔位置覆盖了导叶进出口截面和主线截面。压力恢复系数的理论值和试验值表明当流量大于导叶设计流量时导叶内产生了较大的静压损失。2C/2D PIV 可视化试验的研究结果表明大流量下导叶叶片压力面发生了流动分离。设计了不同参数组合的涡流发生器来对流动分离现象进行控制,试验研究了6种方案对模型泵大流量工况下性能的影响,获得一组最佳方案,并分析了采用该方案后导叶内的流动特性。     

Preservation of martian organic and environmental records: final report of the Mars biosignature working group

The Mars Science Laboratory (MSL) has an instrument package capable of making measurements of past and present environmental conditions. The data generated may tell us if Mars is, or ever was, able to support life. However, the knowledge of Mars' past history and the geological processes most likely to preserve a record of that history remain sparse and, in some instances, ambiguous. Physical, chemical, and geological processes relevant to biosignature preservation on Earth, especially under conditions early in its history when microbial life predominated, are also imperfectly known. Here, we present the report of a working group chartered by the Co-Chairs of NASA's MSL Project Science Group, John P. Grotzinger and Michael A. Meyer, to review and evaluate potential for biosignature formation and preservation on Mars. Orbital images confirm that layered rocks achieved kilometer-scale thicknesses in some regions of ancient Mars. Clearly, interplays of sedimentation and erosional processes govern present-day exposures, and our understanding of these processes is incomplete. MSL can document and evaluate patterns of stratigraphic development as well as the sources of layered materials and their subsequent diagenesis. It can also document other potential biosignature repositories such as hydrothermal environments. These capabilities offer an unprecedented opportunity to decipher key aspects of the environmental evolution of Mars' early surface and aspects of the diagenetic processes that have operated since that time. Considering the MSL instrument payload package, we identified the following classes of biosignatures as within the MSL detection window: organism morphologies (cells, body fossils, casts), biofabrics (including microbial mats), diagnostic organic molecules, isotopic signatures, evidence of biomineralization and bioalteration, spatial patterns in chemistry, and biogenic gases. Of these, biogenic organic molecules and biogenic atmospheric gases are considered the most definitive and most readily detectable by MSL.     

Thyrotropin receptor and membrane interactions in FRTL-5 thyroid cell strain in microgravity

The aim of this work was to analyze the possible alteration of thyrotropin (TSH) receptors in microgravity, which could explain the absence of thyroid cell proliferation in the space environment. Several forms of the TSH receptor are localized on the plasma membrane associated with caveolae and lipid rafts. The TSH regulates the fluidity of the cell membrane and the presence of its receptors in microdomains that are rich in sphingomyelin and cholesterol. TSH also stimulates cyclic adenosine monophosphate (cAMP) accumulation and cell proliferation. Reported here are the results of an experiment in which the FRTL-5 thyroid cell line was exposed to microgravity during the Texus-44 mission (launched February 7, 2008, from Kiruna, Sweden). When the parabolic flight brought the sounding rocket to an altitude of 264?km, the culture media were injected with or without TSH in the different samples, and weightlessness prevailed on board for 6 minutes and 19 seconds. Control experiments were performed, in parallel, in an onboard 1g centrifuge and on the ground in Kiruna laboratory. Cell morphology and function were analyzed. Results show that in microgravity conditions the cells do not respond to TSH treatment and present an irregular shape with condensed chromatin, a modification of the cell membrane with shedding of the TSH receptor in the culture medium, and an increase of sphingomyelin-synthase and Bax proteins. It is possible that real microgravity induces a rearrangement of specific sections of the cell membrane, which act as platforms for molecular receptors, thus influencing thyroid cell function in astronauts during space missions.     

Preservation of microbial lipids in geothermal sinters

Lipid biomarkers are widely used to study the earliest life on Earth and have been invoked as potential astrobiological markers, but few studies have assessed their survival and persistence in geothermal settings. Here, we investigate lipid preservation in active and inactive geothermal silica sinters, with ages of up to 900 years, from Champagne Pool, Waiotapu, New Zealand. Analyses revealed a wide range of bacterial biomarkers, including free and bound fatty acids, 1,2-di-O-alkylglycerols (diethers), and various hopanoids. Dominant archaeal lipids include archaeol and glycerol dialkyl glycerol tetraethers (GDGTs). The predominance of generally similar biomarker groups in all sinters suggests a stable microbial community throughout Champagne Pool's history and indicates that incorporated lipids can be well preserved. Moreover, subtle differences in lipid distributions suggest that past changes in environmental conditions can be elucidated. In this case, higher archaeol abundances relative to the bacterial diethers, a greater proportion of cyclic GDGTs, the high average chain length of the bacterial diethers, and greater concentrations of hopanoic acids in the older sinters all suggest hotter conditions at Champagne Pool in the past.     

DIRECT NUMERICAL SIMULATIONS OF MULTIPHASE FLOWS

INTRODUCTIONMultiphase and multifluid flows are commonin many natural and technologically importantprocesses. Rain,spray combustion,spray paint-ing,and boiling heat transfer are just a few ex-amples.While it is the overall,integral charac-teristics of such flows that are of most interest,the global behavior is determined to a large de-gree by the evolution of the smallestscales in theflow.The combustion of sprays,for example,depends on the size and the number density ofthe drops.Generally…     

Some ecological mechanisms to generate habitability in planetary subsurface areas by chemolithotrophic communities: the Río Tinto subsurface ecosystem as a model system

Chemolithotrophic communities that colonize subsurface habitats have great relevance for the astrobiological exploration of our Solar System. We hypothesize that the chemical and thermal stabilization of an environment through microbial activity could make a given planetary region habitable. The MARTE project ground-truth drilling campaigns that sampled cryptic subsurface microbial communities in the basement of the Río Tinto headwaters have shown that acidic surficial habitats are the result of the microbial oxidation of pyritic ores. The oxidation process is exothermic and releases heat under both aerobic and anaerobic conditions. These microbial communities can maintain the subsurface habitat temperature through storage heat if the subsurface temperature does not exceed their maximum growth temperature. In the acidic solutions of the Río Tinto, ferric iron acts as an effective buffer for controlling water pH. Under anaerobic conditions, ferric iron is the oxidant used by microbes to decompose pyrite through the production of sulfate, ferrous iron, and protons. The integration between the physical and chemical processes mediated by microorganisms with those driven by the local geology and hydrology have led us to hypothesize that thermal and chemical regulation mechanisms exist in this environment and that these homeostatic mechanisms could play an essential role in creating habitable areas for other types of microorganisms. Therefore, searching for the physicochemical expression of extinct and extant homeostatic mechanisms through physical and chemical anomalies in the Mars crust (i.e., local thermal gradient or high concentration of unusual products such as ferric sulfates precipitated out from acidic solutions produced by hypothetical microbial communities) could be a first step in the search for biological traces of a putative extant or extinct Mars biosphere.     

Ultrastructural study of iron oxide precipitates: implications for the search for biosignatures in the Meridiani hematite concretions, Mars

Two terrestrial environments that have been proposed as analogs for the iron oxide precipitation in the Meridiani Planum region of Mars include the Rio Tinto precipitates and southern Utah marble concretions. Samples of two typical Utah iron oxide concretions and iron oxide precipitates in contact with biofilms from Rio Tinto have been studied to determine whether evidence could be found for biomediation in the precipitation process and to identify likely locations for fossil microorganisms. Scanning electron microscopy, energy dispersive X-ray, and gas chromatography-mass spectrometry (GC-MS) were used to search for biosignatures in the Utah marbles. The precipitation of iron oxides resembles known biosignatures, though organic compounds could not be confirmed with GC-MS analysis. In contrast, textural variations induced by biological activity are abundant in the modern Rio Tinto samples. Although no compelling evidence of direct or indirect biomediation was found in the Utah marbles, the ultrastructure of the iron oxide cement in the concretion suggests an inward growth during concretion precipitation from an initially spherical redox front. No indication for growth from a physical nucleus was found.     

The Physical Foundation of Forecasting Disturbances in the Geospace From Solar Disk Characteristics

The five main types of antisunward propagating energetic fluxes (particles and emission) may be thought of as well established to date, the effects of which lead to a particilar character of disturbance in the near-terrestrial environment (the Earth's magnetosphere, ionosphere and atmosphere). The strongest global restructuring of the magnetosphere and ionosphere is caused by fluxes of relatively dense n of 1-70 cm-3 at the Earth's orbit) Solar Wind (SW) quasi-neutral, low-energy (E ＜ 10 keV) plasma which cause magnetospheric and ionospheric storms lasting 24 hours or longer. For that reason, main attention is given to their study at the initial stage of research. The physical essence of the method of predicting disturbances in the near-terrestrial space environment, the amplitude of which can be expressed in, for example, the Kp index units, involves:(1) identifying all the most geo-effective SW streams of type, (2) determing their sources on the solar disk,and (3) quantifying the correlations between the characteristics of their solar sources with a maximum value of the Kp-index that is caused by the concerned type of SW stream. Semi-phenomenological relations have been obtained, which relate parameters of type SW stream sources to characteristics of geomagnetic storms:storm commencement, the time at which the storm intensity reaches its maximum values, the storm duration,as well as to the storm amplitude expressed in terms of geomagnetic indeces.      

喷丸硬化产生的压应力在弯曲疲劳和接触疲劳状态下的稳定性

对于时效前或时效后经过喷丸硬化处理的 Al-Cu-Mg(2014A)铝合金进行了平面弯曲疲劳和接触疲劳试验。时效后进行喷丸硬化处理,改善了高周循环范围内的平面弯曲疲劳和接触疲劳性能。时效前进行喷丸硬化处理,改善了低周循环范围内的平面弯曲疲劳和接触疲劳性能。试验过程中,尤其是在接触疲劳试验状态下,喷丸硬化产生的表面压应力下降,时效前的喷丸硬化处理可使这种下降明显减少,说明了为什么这种处理可以改善低周循环范围内的疲劳性能。