Bacterial growth at the high concentrations of magnesium sulfate found in martian soils

The martian surface environment exhibits extremes of salinity, temperature, desiccation, and radiation that would make it difficult for terrestrial microbes to survive. Recent evidence suggests that martian soils contain high concentrations of MgSO? minerals. Through warming of the soils, meltwater derived from subterranean ice-rich regolith may exist for an extended period of time and thus allow the propagation of terrestrial microbes and create significant bioburden at the near surface of Mars. The current report demonstrates that halotolerant bacteria from the Great Salt Plains (GSP) of Oklahoma are capable of growing at high concentrations of MgSO? in the form of 2 M solutions of epsomite. The epsotolerance of isolates in the GSP bacterial collection was determined, with 35% growing at 2 M MgSO?. There was a complex physiological response to mixtures of MgSO? and NaCl coupled with other environmental stressors. Growth also was measured at 1 M concentrations of other magnesium and sulfate salts. The complex responses may be partially explained by the pattern of chaotropicity observed for high-salt solutions as measured by agar gelation temperature. Select isolates could grow at the high salt concentrations and low temperatures found on Mars. Survival during repetitive freeze-thaw or drying-rewetting cycles was used as other measures of potential success on the martian surface. Our results indicate that terrestrial microbes might survive under the high-salt, low-temperature, anaerobic conditions on Mars and present significant potential for forward contamination. Stringent planetary protection requirements are needed for future life-detection missions to Mars.     

Survival of Bacillus pumilus spores for a prolonged period of time in real space conditions

To prevent forward contamination and maintain the scientific integrity of future life-detection missions, it is important to characterize and attempt to eliminate terrestrial microorganisms associated with exploratory spacecraft and landing vehicles. Among the organisms isolated from spacecraft-associated surfaces, spores of Bacillus pumilus SAFR-032 exhibited unusually high resistance to decontamination techniques such as UV radiation and peroxide treatment. Subsequently, B. pumilus SAFR-032 was flown to the International Space Station (ISS) and exposed to a variety of space conditions via the European Technology Exposure Facility (EuTEF). After 18 months of exposure in the EXPOSE facility of the European Space Agency (ESA) on EuTEF under dark space conditions, SAFR-032 spores showed 10-40% survivability, whereas a survival rate of 85-100% was observed when these spores were kept aboard the ISS under dark simulated martian atmospheric conditions. In contrast, when UV (>110?nm) was applied on SAFR-032 spores for the same time period and under the same conditions used in EXPOSE, a ～7-log reduction in viability was observed. A parallel experiment was conducted on Earth with identical samples under simulated space conditions. Spores exposed to ground simulations showed less of a reduction in viability when compared with the "real space" exposed spores (～3-log reduction in viability for "UV-Mars," and ～4-log reduction in viability for "UV-Space"). A comparative proteomics analysis indicated that proteins conferring resistant traits (superoxide dismutase) were present in higher concentration in space-exposed spores when compared to controls. Also, the first-generation cells and spores derived from space-exposed samples exhibited elevated UVC resistance when compared with their ground control counterparts. The data generated are important for calculating the probability and mechanisms of microbial survival in space conditions and assessing microbial contaminants as risks for forward contamination and in situ life detection.     

Calcification and silicification: fossilization potential of cyanobacteria from stromatolites of Niuafo'ou's Caldera Lakes (Tonga) and implications for the early fossil record

Calcification and silicification processes of cyanobacterial mats that form stromatolites in two caldera lakes of Niuafo'ou Island (Vai Lahi and Vai Si'i) were evaluated, and their importance as analogues for interpreting the early fossil record are discussed. It has been shown that the potential for morphological preservation of Niuafo'ou cyanobacteria is highly dependent on the timing and type of mineral phase involved in the fossilization process. Four main modes of mineralization of cyanobacteria organic parts have been recognized: (i) primary early postmortem calcification by aragonite nanograins that transform quickly into larger needle-like crystals and almost totally destroy the cellular structures, (ii) primary early postmortem silicification of almost intact cyanobacterial cells that leave a record of spectacularly well-preserved cellular structures, (iii) replacement by silica of primary aragonite that has already recrystallized and obliterated the cellular structures, (iv) occasional replacement of primary aragonite precipitated in the mucopolysaccharide sheaths and extracellular polymeric substances by Al-Mg-Fe silicates. These observations suggest that the extremely scarce earliest fossil record may, in part, be the result of (a) secondary replacement by silica of primary carbonate minerals (aragonite, calcite, siderite), which, due to recrystallization, had already annihilated the cellular morphology of the mineralized microbiota or (b) relatively late primary silicification of already highly degraded and no longer morphologically identifiable microbial remains.     

A new spectral index to detect Poaceae grass abundance in Mongolian grasslands

The objectives of the present study were to develop a new index based on remotely-sensed data for detecting the abundance of grasses in the family Poaceae, which has a high palatability for livestock in Mongolia, and to map the distribution of these grasses in the semi-arid Mongolian steppes. We measured ground-based spectral reflectance of pure plant leaves – including Poaceae grasses – and soils, as well as in-situ in the Mongolian grasslands. The hyper-spectral data, taken by a spectroradiometer, were converted into four multi-spectral bands (i.e., blue, green, red, and NIR) to simulate satellite-based imagery data. In order to magnify the characteristics of the spectral signal of Poaceae, NGBDI (Normalized Green-Blue Difference Index), NGRDI (Normalized Green-Red Difference Index), NDVI (Normalized Difference Vegetation Index), NNBDI (Normalized NIR-Blue Difference Index) were calculated from the four multi-spectral reflectance values. Poaceae Abundance Index (PAI) was derived by combining these four normalized difference indices. PAI was found out to be a good indicator to discriminate Poaceae grass from the other plant spectral data.     

Comparison of Antarctic riometer radio wave absorption and THEMIS mission energetic electron fluxes

Simultaneous observations of in situ plasma properties in the tail of the Earth’s magnetosphere and of ground based instruments, lying on the same geomagnetic field lines, have recently proved to yield significant new results. In most cases magnetosphere ionosphere interactions during the night-time northern hemisphere conditions are studied. Here, observations of energetic electrons in the tail of the Earth’s magnetosphere made by the THEMIS mission satellites are compared with auroral radio wave absorption determined by riometers in the Antarctic for sunlit conditions. Days for which satellites and riometers are connected by the same geomagnetic field line are selected using a geomagnetic field model. The six days analysed show clear associations between fluxes and absorptions in some cases. However, these do not necessarily correspond to conjugacy intervals. Hours of positive associations are 1.65 times those for negative associations, all hours and days considered (1.42–3.6 on five days and 0.58 on the other day). These computations are assumed appropriate since the footprints of the satellites used approximately follow corrected geomagnetic parallels for all six days studied. The use of a finer parameterization of geomagnetic models to determine conjugacy may be needed.     

GNSS-R concept extended by a fine orbit tuning

Small changes in semimajor axis of the orbits selected for the GNSS-R [R as Reflectometry] satellites, so-called fine orbit tuning, known from the ESA’s Gravity and steady-state Ocean Circulation Explorer mission, can dramatically increase the number of nadir and off-nadir reflecting points and, in turn, can enhance the capability of the concept of bistatic altimetry (GNSS Reflectometry) without additional costs. The application of our suggestion is feasible for a satellite which will be equipped by thrusters for the orbit keeping. During the mission lifetime several orbit tunings are feasible, just to transfer from one to another orbit. Then we can study short-periodic or longer-periodic features, according to scientific goals defined for the mission. The shortest cycles (few days), corresponding to the required revisit time (defined by ESA), may be subcycles of much longer cycles (repeat periods).     

Seasonal-to-interannual variability of chlorophyll-a bloom timing associated with physical forcing in the Gulf of Cádiz

Seasonal-to-interannual variability of the winter-spring bloom in the Gulf of Cádiz, eastern North Atlantic, has been investigated using chlorophyll-a remote sensing (CHL). These data have been obtained from the GlobColour project; the temporal coverage extends from September 1997 to December 2010. In this study we develop a generic quantitative approach for describing the temporal variability in the shape of the winter-spring bloom within a region. Variability in both the timing and magnitude of the bloom in the basin has been evaluated as a function of physical properties in the water column such as Mixed Layer Depth (MLD, GODAS model), sea surface temperature (SST, from AVHRR radiometers), photosynthetically-active radiation (PAR, from ocean color data) and euphotic depth (Z_eu, from ocean color data). The analysis indicated that the timing, size and duration of the phytoplankton bloom in this area are largely controlled by both meteorological and oceanographic conditions at different scales; this means that it is likely to vary widely from one year to another.     

Planetary Magnetic Fields: Achievements and Prospects

The past decade has seen a wealth of new data, mainly from the Galilean satellites and Mars, but also new information on Mercury, the Moon and asteroids (meteorites). In parallel, there have been advances in our understanding of dynamo theory, new ideas on the scaling laws for field amplitudes, and a deeper appreciation on the diversity and complexity of planetary interior properties and evolutions. Most planetary magnetic fields arise from dynamos, past or present, and planetary dynamos generally arise from thermal or compositional convection in fluid regions of large radial extent. The relevant electrical conductivities range from metallic values to values that may be only about one percent or less that of a typical metal, appropriate to ionic fluids and semiconductors. In all planetary liquid cores, the Coriolis force is dynamically important. The maintenance and persistence of convection appears to be easy in gas giants and ice-rich giants, but is not assured in terrestrial planets because the quite high electrical conductivity of an iron-rich core guarantees a high thermal conductivity (through the Wiedemann-Franz law), which allows for a large core heat flow by conduction alone. This has led to an emphasis on the possible role of ongoing differentiation (growth of an inner core or “snow”). Although planetary dynamos mostly appear to operate with an internal field that is not very different from (2ρΩ/σ)^1/2 in SI units where ρ is the fluid density, Ω is the planetary rotation rate and σ is the conductivity, theoretical arguments and stellar observations suggest that there may be better justification for a scaling law that emphasizes the buoyancy flux. Earth, Ganymede, Jupiter, Saturn, Uranus, Neptune, and probably Mercury have dynamos, Mars has large remanent magnetism from an ancient dynamo, and the Moon might also require an ancient dynamo. Venus is devoid of a detectable global field but may have had a dynamo in the past. Even small, differentiated planetesimals (asteroids) may have been capable of dynamo action early in the solar system history. Induced fields observed in Europa and Callisto indicate the strong likelihood of water oceans in these bodies. The presence or absence of a dynamo in a terrestrial body (including Ganymede) appears to depend mainly on the thermal histories and energy sources of these bodies, especially the convective state of the silicate mantle and the existence and history of a growing inner solid core. As a consequence, the understanding of planetary magnetic fields depends as much on our understanding of the history and material properties of planets as it does on our understanding of the dynamo process. Future developments can be expected in our understanding of the criterion for a dynamo and on planetary properties, through a combination of theoretical work, numerical simulations, planetary missions (MESSENGER, Juno, etc.) and laboratory experiments.     

Microbial fuel cells applied to the metabolically based detection of extraterrestrial life

Since the 1970s, when the Viking spacecrafts carried out experiments to detect microbial metabolism on the surface of Mars, the search for nonspecific methods to detect life in situ has been one of the goals of astrobiology. It is usually required that a methodology detect life independently from its composition or form and that the chosen biological signature point to a feature common to all living systems, such as the presence of metabolism. In this paper, we evaluate the use of microbial fuel cells (MFCs) for the detection of microbial life in situ. MFCs are electrochemical devices originally developed as power electrical sources and can be described as fuel cells in which the anode is submerged in a medium that contains microorganisms. These microorganisms, as part of their metabolic process, oxidize organic material, releasing electrons that contribute to the electric current, which is therefore proportional to metabolic and other redox processes. We show that power and current density values measured in MFCs that use microorganism cultures or soil samples in the anode are much larger than those obtained with a medium free of microorganisms or sterilized soil samples, respectively. In particular, we found that this is true for extremophiles, which have been proposed as potential inhabitants of extraterrestrial environments. Therefore, our results show that MFCs have the potential to be used for in situ detection of microbial life.