Host–parasite interactions in closed and open microbial cultivation system

The study addresses interaction of bacteria and phages in the host–parasite system in batch and continuous cultures. The study system consists of the auxotrophic strain of Brevibacterium – Brevibacterium sp. 22L – and the bacteriophage of Brevibacterium sp., isolated from the soil by the enrichment method.

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Closed system. In the investigation of the relationship between the time of bacterial lysis and multiplicity of phage infection it has been found that at a lower phage amount per cell it takes a longer time for the lysis of the culture to become discernible. Another important factor determining cytolysis in liquid medium is the physiological state of bacterial population. Specific growth rate of bacteria at the moment of phage infection has been chosen as an indicator of the physiological state of bacteria. It has been shown that the shortest latent period and the largest output of the phage are observed during the logarithmic growth phase of bacteria grown under favorable nutrient conditions. In the stationary phase, bacterial cells become “a bad host” for the phage, whose reproduction rate decreases, and the lysis either slows down significantly or does not occur at all.     

High resolution science with high redshift galaxies

We summarize the high-resolution science that has been done on high redshift galaxies with Adaptive Optics (AO) on the world’s largest ground-based facilities and with the Hubble Space Telescope (HST). These facilities complement each other. Ground-based AO provides better light gathering power and in principle better resolution than HST, giving it the edge in high spatial resolution imaging and high resolution spectroscopy. HST produces higher quality, more stable PSF’s over larger field-of-views in a much darker sky-background than ground-based AO, and yields deeper wide-field images and low-resolution spectra than the ground. Faint galaxies have steadily decreasing sizes at fainter fluxes and higher redshifts, reflecting the hierarchical formation of galaxies over cosmic time. HST has imaged this process in great structural detail to z  6, and ground-based AO and spectroscopy has provided measurements of their masses and other physical properties with cosmic time. Last, we review how the 6.5 m James Webb Space Telescope (JWST) will measure First Light, reionization, and galaxy assembly in the near–mid-IR after 2013.     

Aerosols and clouds in the upper troposphere–lower stratosphere region detected by GOMOS and ACE: Intercomparison and analysis of the years 2004 and 2005

Satellite-based limb occultation measurements are well suited for the detection and mapping of polar stratospheric clouds (PSCs) and cirrus clouds. PSCs are of fundamental importance for the formation of the Antarctic ozone hole that occurs every year since the early 1980s in Southern Hemisphere spring. Despite progress in the observation, modeling and understanding of PSCs in recent years, there are still important questions which remain to be resolved, e.g. PSC microphysics, composition, formation mechanisms and long-term changes in occurrence. In addition, it has recently become clear that cirrus clouds significantly affect the global energy balance and climate, due to their influence on atmospheric thermal structure.     

Semi-analytical investigations of high area-to-mass ratio geosynchronous space debris including Earth’s shadowing effects

This paper investigates the long-term perturbations of the orbits of geosynchronous space debris influenced by direct radiation pressure including the Earth’s shadowing effects. For this purpose, we propose an extension of our homemade semi-analytical theory [Valk, S., Lemaître, A., Deleflie, F. Semi-analytical theory of mean orbital motion for geosynchronous space debris under gravitational influence. Adv. Space Res., submitted for publication], based on the method developed by Aksnes [Aksnes, K. Short-period and long-period perturbations of a spherical satellite due to direct solar radiation. Celest. Mech. Dyn. Astron. 13, 89–104, 1976] and generalized into a more convenient non-singular formalism. The perturbations accounting for the direct radiation pressure with the Earth’s shadow are computed on a revolution-by-revolution basis, retaining the original osculating Hamiltonian disturbing function. In this framework, we compute the non-singular mean longitude at shadow entry and shadow exit at every orbital revolution in opposition to classical approaches where the singular eccentric anomalies at shadow entry and shadow exit are computed. This new algorithm is developed using non-singular variables. Consequently, it is particularly suitable for both near-circular and near-equatorial orbits as well as orbits which transit periodically around null eccentricities and null inclinations.The algorithm is tested by means of numerical integrations of the equations, averaged over the short periods, including radiation pressure, J₂, the combined Moon and Sun third body attraction as well as the long-term effects of the 1:1 resonance occurring for geosynchronous objects. As an extension of [Valk, S., Lemaître, A., Anselmo, L. Analytical and semi-analytical investigations of geosynchronous space debris with high area-to-mass ratios influenced by solar radiation pressure. Adv. Space Res., doi:10.1016/j.asr.2007.10.025, 2007b], we especially apply our analysis to space debris with area-to-mass as high as 20 m²/kg. This paper provides numerical and semi-analytical investigations leading to a deep understanding of the long-term evolution of the semi-major axis. Finally, these semi-analytical investigations are compared with accurate numerical integrations of the osculating equations of motion over time scales as high as 25 years.     

Interstellar planetary protection

In the coming decades the detection of Earth-like extrasolar planets, either apparently lifeless or exhibiting spectral signatures of life, will encourage design studies for craft to visit them. These missions will require the elaboration of an interstellar planetary protection protocol. Given a specific dose required to sterilize microorganisms on a spacecraft, a critical mean velocity can be determined below which a craft becomes self-sterilizing. This velocity is calculated to be below velocities previously projected for interstellar missions, suggesting that an active sterilization protocol prior to launch might be required. Given uncertainties in the surface conditions of a destination extrasolar planet, particularly at microscopic scales, the potential for unknown biochemistries and biologies elsewhere, or the possible inoculation of a lifeless planet that is habitable, then both lander and orbiter interstellar missions should be completely free of all viable organisms, necessitating a planetary protection approach applied to orbiters and landers bound for star systems with unknown local conditions for habitability. I discuss the case of existing craft on interstellar trajectories – Pioneer 10, 11 and Voyager 1 and 2.     

The Dynamic Albedo of Neutrons (DAN) experiment for NASA's 2009 Mars Science Laboratory

We present a summary of the physical principles and design of the Dynamic Albedo of Neutrons (DAN) instrument onboard NASA's 2009 Mars Science Laboratory (MSL) mission. The DAN instrument will use the method of neutron-neutron activation analysis in a space application to study the abundance and depth distribution of water in the martian subsurface along the path of the MSL rover.     

Numerical simulation of effects of the August 11, 1999 solar eclipse in the outer ionosphere

The results of a numerical simulation of such parameters of the topside ionosphere as concentration N _e and temperature T _e of electrons, and concentration n(H⁺) and fluxes along the magnetic field lines Φ(H⁺) of H⁺ ions at an altitude of ～2000 km for the conditions of the August 11, 1999 solar eclipse are presented. The calculations were performed using the Global Self-consistent Model of the Thermosphere, Ionosphere, and Protonosphere of the Earth (GSM TIP). It is shown that during the eclipse, in addition to a region of decreased values of T _e in the Northern Hemisphere and in the magnetically conjugate region in the Southern Hemisphere, regions of electron heating emerge in both hemispheres. Simultaneously, an extended region of decreased values of N _e comes into existence and moves behind the Moon’s shadow. Regions with decreased (down to ～30%) and enhanced (up to ～50%) concentrations of H⁺ ions are detected in the global distribution of these ions.     

Searching for life on Mars: selection of molecular targets for ESA's aurora ExoMars mission

The European Space Agency's ExoMars mission will seek evidence of organic compounds of biological and non-biological origin at the martian surface. One of the instruments in the Pasteur payload may be a Life Marker Chip that utilizes an immunoassay approach to detect specific organic molecules or classes of molecules. Therefore, it is necessary to define and prioritize specific molecular targets for antibody development. Target compounds have been selected to represent meteoritic input, fossil organic matter, extant (living, recently dead) organic matter, and contamination. Once organic molecules are detected on Mars, further information is likely to derive from the detailed distribution of compounds rather than from single molecular identification. This will include concentration gradients beneath the surface and gradients from generic to specific compounds. The choice of biomarkers is informed by terrestrial biology but is wide ranging, and nonterrestrial biology may be evident from unexpected molecular distributions. One of the most important requirements is to sample where irradiation and oxidation are minimized, either by drilling or by using naturally excavated exposures. Analyzing regolith samples will allow for the search of both extant and fossil biomarkers, but sequential extraction would be required to optimize the analysis of each of these in turn.     

Influence on photosynthesis of starlight, moonlight, planetlight, and light pollution (reflections on photosynthetically active radiation in the universe)

Photosynthesis on Earth can occur in a diversity of organisms in the photosynthetically active radiation (PAR) range of 10 nmol of photons m(-2) s(-1) to 8 mmol of photons m(-2) s(-1). Similar considerations would probably apply to photosynthetic organisms on Earth-like planets (ELPs) in the continuously habitable zone of other stars. On Earth, starlight PAR is inadequate for photosynthetically supported growth. An increase in starlight even to reach the minimum theoretical levels to allow for photosynthesis would require a universe that was approximately ten million times older, or with a ten million times greater density of stars, than is the case for the present universe. Photosynthesis on an ELP using PAR reflected from a natural satellite with the same size as our Moon, but at the Roche limit, could support a low rate of photosynthesis at full Moon. Photosynthesis on an ELP-like satellite of a Jupiter-sized planet using light reflected from the planet could be almost 1% of the rate in full sunlight on Earth when the planet was full. These potential contributions to photosynthesis require that the contribution is compared with the rate of photosynthesis driven by direct radiation from the star. Light pollution on Earth only energizes photosynthesis by organisms that are very close to the light source. However, effects of light pollution on photosynthesis can be more widespread if the photosynthetic canopy is retained for more of the year, caused by effects on photoperiodism, with implications for the influence of civilizations on photosynthesis.