Assessment of the efficacy of medical countermeasures in space flight

Changes in body fluids, electrolytes, and muscle mass are manifestations of adaptation to space flight and readaptation to the 1-g environment. The purposes of this paper are to review the current knowledge of biomedical responses to short- and long-duration space missions and to assess the efficacy of countermeasures to 1-g conditioning. Exercise protocols, fluid hydration, dietary and potential pharmacologic measures are evaluated, and directions for future research activities are recommended.     

SETI at the Nancay radiotelescope

The Nancay (France) radiotelescope has been used in June, 1981, to search for artificial monochromatic signals from 102 nearby stars, without success. A different approach to SETI is also considered based on the properties of wide band signals. A detection procedure, through Karhunen-Loeve analysis, is suggested.     

用振动数据确定梁柱屈曲载荷

引言对某一特定结构,人们可以用简单的振动试验确定其固有频率。我们知道,在数学上固有频率是结构边界条件的函数;同样,临界屈曲载荷亦是结构支承边界条件的函数。有些结构需要现场制作或者在使用之前对它们的支承边界条件一无所知。这些情况常常希望用无损试验预示使用条件下的屈曲载荷。对于笨重的结构,作静力或屈曲破坏试验有困难,而作固     

焊接规范中的断裂控制应用

一、前 言 一般,宇航工业典型焊接规范对缺陷的检验是非常保守的。诸如裂纹、夹杂、未焊透等缺陷,在载人舱事实上几乎是不允许的。本文旨在介绍航天飞机推进剂壳体焊接检验方面的研究,即应用断裂控制来确立焊接规范可容许缺陷的准则。此外,还将讨论焊接特性对验证试验条件的影响规律。     

提高航线维护效率的管理工具

由于担心扰乱现有的航线运营,往往忽视了航线维护管理模式的改进.但也有一些航空公司将航线维护管理的改进看作是彻底改变运营模式,获得新的竞争力的机会.     

The ankle ergometer: a new tool for quantifying changes in mechanical properties of human muscle as a result of spaceflight

A mechanical device for studying changes in mechanical properties of human muscle as a result of spaceflight is presented. Its main capacities are to allow during a given experiment investigation of both contractile and visco-elastic properties of a musculo-articular complex using respectively isometric contractions, isokinetic movements, quick-release tests and sinusoidal perturbations. This device is a motor driven ergometer associated to an experimental protocol designed for pre- and post-flight experiments. As microgravity preferentially affects postural muscles, the apparatus was designed to test muscle groups crossing the ankle joint. Three subjects were tested during the Euromir '94 mission. Preliminary results obtained on the european astronaut are briefly reported. During the next two years the experiments will be performed during six missions.     

Separation of bacterial cells by free flow electrophoresis under microgravity: a result of the SpaceLab-Japan project on Space Shuttle flight STS-47

We demonstrated free flow electrophoresis (FFE) of charged cells under microgravity, where gravitational effects are almost eliminated. Separation of a mixture of three bacterial strains (mutants of Salmonella typhimurium LT2) by FFE was conducted on NASA Space Shuttle flight STS-47 (September 1992). The experiment was designed to differentiate three strains having different lipopolysaccharide core structures in the cell membrane. The results were compared to those of ground experiments, in order to examine whether or not FFE in a weightless environment provides distinct advantages. Smooth strain SL1027 and rough strain SL3749 migrated to two separated fractions. The quality (viability) and the yields of the separated samples were sufficient to show the advantage of microgravity. Another rough strain, SL1102, exhibited unexpected electrophoretic behavior, which prevented the complete resolution of the three strains. All the strains were recovered as viable cells after 8 days of flight. The present study suggests that electrophoretic separation of bacterial cells is much more effective under microgravity conditions with relatively good resolution in comparison with the ground operation.     

EDEN: a payload dedicated to neurovestibular research for Neurolab

The European Space Agency contributes to the Neurolab mission through the delivery of the ESA Developed Elements for Neurolab (EDEN). Those elements include one set supporting the Autonomic Nervous System experiment and one set supporting the Neurovestibular (so-called ATLAS) experiment. This second set is called the Visual and Vestibular Investigation System (VVIS). This paper describes the main characteristics of the VVIS and its various subsystems. The scientific objectives and operational constraints of the ATLAS experiment to be carried out with this equipment during Neurolab are presented to underline the correspondence between the VVIS design and the scientific requirements. Further scientific and technical perspectives for the VVIS, particularly within the scope of the International Space station, are also proposed.     

Taxane recovery from cells of Taxus in micro- and hypergravity

Cell suspension cultures of Taxus cuspidata produce taxanes that are released from the outer surface of cells into the culture medium as free and bound alkaloids. Paclitaxel (Taxol (TM)), is an anti-cancer drug in short supply. It has a taxane ring derived from baccatin III and a C-13 phenylisoserine side-chain. This drug is produced over a wide range of gravitational forces. Monoclonal and polyclonal antibodies to paclitaxel, baccatin III, and the C-13 phenylisoserine side chain were combined in multiple-labeling studies to localize taxanes and paclitaxel on cell surfaces or on particles released into the culture medium. Bioreactor vessel design altered the composition of taxanes recovered from cells in simulated microgravity. At 10(-2) and 2x10(-4)g, taxane recovery was reduced but biomass growth and percent paclitaxel was significantly increased. At 1 to 24g, growth was reduced with a significant recovery of total taxanes with low percent paclitaxel. Bound paclitaxel was also localized in endonuclease-rich fragmenting nuclei of individual apoptotic cells. A model is presented comprising TCH (touch) genes encoding enzymes that modify taxane-bearing xylan residues in cell walls, the calcium-sensing of gravitational forces by the cytoplasm, and the predisposition of nuclei to apoptosis. This integrates the adaptive physiological and biochemical responses of drug-producing genomes with gravitational forces.