Induction of genomic instability after an acute whole-body exposure of mice to Fe ions

The purpose of this study was to evaluate dose–response relationships for the in vivo induction of micronuclei (MN) as a measure of both initial radiation damage and the induction of genomic instability. These measurements were made in mouse blood erythrocytes as a function of radiation dose, radiation quality, time after irradiation, and the genetic background of exposed individuals. Blood samples were collected from two strains of mouse (CBA/CaJ and C57BL/6J) at different times up to 3 months following a whole-body exposure to various doses of 1 GeV/amu ⁵⁶Fe ions (0, 0.1, 0.5 and 1.0 Gy, at the dose rate of a 1 Gy/min) or ¹³⁷Cs gamma rays (0, 0.5, 1.0 and 3.0 Gy, at the dose rate of 0.72 Gy/min). Blood-smear slides were stained with acridine orange (AO). The frequencies of MN were measured in mature normochromatic-erythrocytes (MN-NCEs) and in immature polychromatic-erythrocytes (MN-PCEs). Effects of both types of radiation on erythropoiesis were also evaluated. As a measure of cell progression delay, a dose-dependent decrease in numbers of PCEs was observed at day 2 post-exposure in both strains, regardless of radiation quality. Subsequently, the levels of PCEs increased in all exposed mice, reaching control levels (or higher) by day 7 post-exposure. Further, at day 2 after the exposure, there was no increase in the frequency of MN-PCEs in CBA/CaJ mice exposed to ⁵⁶Fe ions while the frequency of MN-PCEs elevated as a function of dose in the C57BL/6J mice. At day 4, there was no dose related increase in MN-NCEs in either strain of mouse exposed to ¹³⁷Cs gamma rays. Additionally, at the early sacrifice times (days 2 and 4), ⁵⁶Fe ions were slightly more effective (per unit dose) in inducing MN-NCEs than ¹³⁷Cs gamma rays in CBA/CaJ mice. However, there was no increase in the frequency of MN-NCEs at late times after an acute exposure to either type of radiation. In contrast, both types of radiation induced increased MN-PCEs frequencies in irradiated CBA/CaJ mice, but not C57BL/6J mice, at late times post-exposure. This finding indicates the potential induction of genomic instability in hematopoietic cells of CBA/CaJ mice by both types of radiation. The finding also demonstrates the influence of genetic background on radiation-induced genomic instability in vivo.     

Improved interpretation of solar wind ion measurements via high-resolution magnetic field data

The Wind   spacecraft’s Faraday cups (FC) continue to produce high-quality, in situ observations of thermal protons (i.e., ionized hydrogen) and α$\alpha$-particles (i.e., fully ionized helium) in the solar wind. By fitting a Wind/FC ion spectrum with a model velocity distribution function (VDF) for each particle species, values for density, bulk velocity, and temperature can be inferred. Incorporating measurements of the background magnetic field from the Wind Magnetic Field Investigation (MFI) allows perpendicular and parallel temperature components to be separated. Prior implementations of this analysis averaged the higher-cadence Wind/MFI measurements to match that of the Wind/FC ion spectra. However, this article summarizes recent and extensive revisions to the analysis software that, among other things, eliminate such averaging and thereby account for variations in the direction of the magnetic field over the time taken to measure the ions. A statistical comparison reveals that the old version consistently underestimates the temperature anisotropy of ion VDF’s: averaging over fluctuations in the magnetic field essentially blurs the perpendicular and parallel temperature components, which makes the plasma seem artificially more isotropic. The new version not only provides a more accurate dataset of ion parameters (which is well suited to the study of microkinetic phenomena), it also demonstrates a novel technique for jointly processing particle and field data. Such methods are crucial to heliophysics as wave-particle interactions are increasingly seen as playing an important role in the dynamics of the solar wind and similar space plasmas.     

French research program on the physiological problems caused by weightlessness. Use of the primate model

The need to acquire a better knowledge of the main biological problems induced by microgravity implies--in addition to human experimentation--the use of animal models, and primates seem to be particularly well adapted to this type of research. The major areas of investigation to be considered are the phospho-calcium metabolism and the metabolism of supporting tissues, the hydroelectrolytic metabolism, the cardiovascular function, awakeness, sleep-awakeness cycles, the physiology of equilibrium and the pathophysiology of space sickness. Considering this program, the Centre d'Etudes et de Recherches de Medecine Aerospatiale, under the sponsorship of the Centre National d'Etudes Spatiales, developed both a program of research on restrained primates for the French-U.S. space cooperation (Spacelab program) and for the French-Soviet space cooperation (Bio-cosmos program), and simulation of the effects of microgravity by head-down bedrest. Its major characteristics are discussed in the study.     

The past and future of the earth-moon system

The lunar orbit is presently expanding due, we believe, to tidal friction, i.e. the attraction of the moon for the tides it raises on the rotating Earth. The Moon may therefore have been significantly closer to the Earth in the distant past, a point of great interest to those studying the lunar origin. This work presents the results of the integration of the equations which govern the rates of change of the lunar orbit elements and the angular momentum of the Earth. Results are presented for both the past and future of the Earth-Moon system.     

Modern aspects of planetary protection and requirements to sterilization of space hardware.

The viewpoint of working group of Russian experts on the problem of planetary protection for future manned and unmanned Mars mission is presented. Recent data of Martian environment and on survival of terrestrial microorganisms in extreme conditions were used for detailed analysis and overview of planetary protection measures in regard to all possible flight situations including accidental landing. The special emphasis on "Mars-94" mission was done. This analysis resulted in revised formulation of spacecraft sterilization requirements and possible measures for their best implementation. New general combined approach to spacecraft sterilization was proposed. It includes penetrating radiation and heat treatment of spacecraft parts and components which is to be carried out before the final assembly of spacecraft and gaseous radiation sterilization of the whole spacecraft during the flight to Mars (or from Mars for return missions).     

Instrumentenentwicklungen für anwendungsaufgaben der fernerkundung photogrammetrie,geophysik und geodäsie

(Instrument Developments for Applications in Remote Sensing, Photogrammetry, Geophysics and Geodesy) The Modular Optoelectronic Multispectral Seanner MOMS-01, a CCD camera using the push-broom scanner principle, is presented in this paper in its flight configuration. This instrument will be the first European-built spaceborne unaging system for remote sensing applications (launch with Shuttle flight No. 7).Follow-on developments of this initial two-channel version are oriented to an extension of capabilities by a panchromatic high-resolution stereoscopic module for thematic mapping and two additional spectral channels in the reflective IR (up to 2.3 μm) offering a high interpretation potential for future earth resources exploration missions. Furthermore the subjects of advanced laser technology studies are introduced: A picosecond pulse laser system for detection of tectonic motions and for geodetic application, a CO laser for ultra precise range rate determination (Earth potential and Geoid) and a laser range finder for intersatellite distance measurements.     

Bubble detector characterization for space radiation

In light of the importance of the neutron contribution to the dose equivalent received by space workers in the near-Earth radiation environment, there is an increasing need for a personal dosimeter that is passive in nature and able to respond to this neutron field in real time. Recent Canadian technology has led to the development of a bubble detector, which is sensitive to neutrons, but insensitive to low linear energy transfer (LET) radiation. By changing the composition of the bubble detector fluid (or “superheat”), the detectors can be fabricated to respond to different types of radiation. This paper describes a preliminary ground-based research effort to better characterize the bubble detectors of different compositions at various charged-particle accelerator facilities, which are capable of simulating the space radiation field.     

Preferred frequencies for SETI observations

SETI observational programs conducted over the last two decades, and most of those planned for the near future, have concentrated on searching for signals at microwave frequencies. Considerations of signal-to-noise ratio at the receiving end indicate that this is the correct approach if the broadcasting society is not concerned with directionality and transmits into a fairly large solid angle. However, if that society desires to transmit only a highly directional beacon, then it is not now possible, given our lack of knowledge of advanced space technology, to predict reliably whether microwave or infrared wavelengths are to be preferred in an optimum search program. Given the realities of current terrestrial technology, either the centimeter or millimeter domain is to be preferred to the infrared, independent of considerations of directionality. In any event, there does not appear to be any cosmically unique (“magic”) frequency at which to conduct SETI.     

COSAC onboard Rosetta: a bioastronomy experiment for the short-period comet 67P/Churyumov-Gerasimenko

The COSAC (Cometary Sampling and Composition Experiment) onboard the Rosetta mission is a combined gas chromatograph (GC)-mass spectrometer (MS). It is situated on Philae, the lander of the mission, which is intended to land on the nucleus of comet 67P/Churyumov- Gerasimenko. The purpose of the experiment is to analyze the volatile fraction of soil samples retrieved by a drill. For investigation, the samples will be pyrolysed, and the emanating gases fed into a GC, into an MS, or the combination of both. In the first part of this paper, the bioastronomical relevance of such measurements is outlined. In the second part the details of the hardware and its performance are described.