Graviperception in ciliates: steps in the transduction chain.

Ciliates represent suitable model systems to study the mechanisms of graviperception and signal transduction as they show clear gravity-induced behavioural responses (gravitaxis and gravikinesis). The cytoplasm seems to act as a "statolith" stimulating mechanosensitive ion channels in the cell membrane. In order to test this hypothesis, electrophysiological studies with Stylonychia mytilus were performed, revealing the proposed changes (de- or hyperpolarization) depending on the cell's spatial orientation. The behaviour of Paramecium and Stylonychia was also analyzed during variable acceleration conditions of parabolic flights (5th German Parabolic Flight Campaign, 2003). The corresponding data confirm the relaxation of the graviresponses in microgravity as well as the existence of thresholds of graviresponses, which are found to be in the range of 0.4xg (gravikinesis) and 0.6xg (gravitaxis).     

DNA structures and radiation injury.

In the present paper experimental results from radiobiological investigations of the sedimentation behaviour of damaged and restored DNA-subunits attached to the nuclear membrane have been summarized. The studies were carried out preferably with Chinese Hamster cells V79-4 irradiated with different kinds of radiation (gamma-rays, neutrons and carbon ions) using the nucleoid sedimentation technique. Single-strand breaks relax the supercoiled DNA in the subunits resulting in a decreased sedimentation velocity. Rejoining leads to a correct restoration of the structure as can be studied by means of postincubation irradiation. Double-strand breaks release DNA fragments, again leading to an increased sedimentation velocity. If the average number of the induced double-strand breaks per subunit increases to a number higher than one, the measured results suggest that the structures should not be restored completely. The results are compatible with a new repair model developed in our laboratory on the assumption that, firstly, the single DNA subunits are the sensitive target rather than the whole DNA and, secondly, the repair of DNA damage takes place independently in each subunit.     

Protozoa as model systems for the study of cellular responses to altered gravity conditions.

The orientation behavior of Paramecium changed in a similar way after transition to conditions of free-fall in a sounding rocket and after transition to conditions of simulated weightlessness on a fast rotating clinostat. After a period of residual orientation, Paramecium cells distributed themselves randomly 80 s (120 s) after onset of free-fall (simulated weightlessness). Swimming velocity increased significantly; however, the increase was transient and subsided after 3 min in the rocket experiments, while the velocity remained enhanced even during 2 h of rotation on a fast clinostat. Trichocysts were present and without morphological changes in Paramecium cells which had been exposed to a rocket flight, as well as to fast or slow rotation on a clinostat. Regeneration of the oral apparatus of Stentor and morphogenesis of Eufolliculina proceeded normally on the clinostat. The results demonstrate that the clinostat is a useful tool to simulate the conditions of weightlessness on earth and to detect gravisensitive cellular functions.     

Fine structure of the interplanetary shock front according to measurements of the ion flux of the solar wind with high time resolution

According to the data of the BMSW/SPEKTR-R instrument, which measured the density and velocity of solar wind plasma with a record time resolution, up to ~3 ×10^–2 s, the structure of the front of interplanetary shocks has been investigated. The results of these first investigations were compared with the results of studying the structure of the bow shocks obtained in previous years. A comparison has shown that the quasi-stationary (averaged over the rapid oscillations) distribution of plasma behind the interplanetary shock front was significantly more inhomogeneous than that behind the bow-shock front, i.e., in the magnetosheath. It has also been shown that, to determine the size of internal structures of the fronts of quasi-perpendicular (θ_BN > 45°) shocks, one could use the magnetic field magnitude, the proton density, and the proton flux of the solar wind on almost equal terms. A comparison of low Mach (М _А < 2), low beta (β₁ < 1) fronts of interplanetary and bow shocks has shown that the dispersion of oblique magnetosonic waves plays an essential role in their formation.     

Proportional fuzzy feed-forward architecture control validation by wind tunnel tests of a morphing wing

In aircraft wing design, engineers aim to provide the best possible aerodynamic performance under cruise flight conditions in terms of lift-to-drag ratio. Conventional control sur-faces such as flaps, ailerons, variable wing sweep and spoilers are used to trim the aircraft for other flight conditions. The appearance of the morphing wing concept launched a new challenge in the area of overall wing and aircraft performance improvement during different flight segments by locally altering the flow over the aircraft's wings. This paper describes the development and appli-cation of a control system for an actuation mechanism integrated in a new morphing wing structure. The controlled actuation system includes four similar miniature electromechanical actuators dis-posed in two parallel actuation lines. The experimental model of the morphing wing is based on a full-scale portion of an aircraft wing, which is equipped with an aileron. The upper surface of the wing is a flexible one, being closed to the wing tip; the flexible skin is made of light composite materials. The four actuators are controlled in unison to change the flexible upper surface to improve the flow quality on the upper surface by delaying or advancing the transition point from laminar to turbulent regime. The actuators transform the torque into vertical forces. Their bases are fixed on the wing ribs and their top link arms are attached to supporting plates fixed onto the flex-ible skin with screws. The actuators push or pull the flexible skin using the necessary torque until the desired vertical displacement of each actuator is achieved. The four vertical displacements of the actuators, correlated with the new shape of the wing, are provided by a database obtained through a preliminary aerodynamic optimization for specific flight conditions. The control system is designed to control the positions of the actuators in real time in order to obtain and to maintain the desired shape of the wing for a specified flight condition. The feasibility and effectiveness of the developed control system by use of a proportional fuzzy feed-forward methodology are demon-strated experimentally through bench and wind tunnel tests of the morphing wing model.     

A high-order SPH method by introducing inverse kernels

The smoothed particle hydrodynamics (SPH) method is usually expected to be an effi-cient numerical tool for calculating the fluid-structure interactions in compressors; however, an endogenetic restriction is the problem of low-order consistency. A high-order SPH method by intro-ducing inverse kernels, which is quite easy to be implemented but efficient, is proposed for solving this restriction. The basic inverse method and the special treatment near boundary are introduced with also the discussion of the combination of the Least-Square (LS) and Moving-Least-Square (MLS) methods. Then detailed analysis in spectral space is presented for people to better under-stand this method. Finally we show three test examples to verify the method behavior.     

准一维传热下冷面绝热边界材料对隔热试验结果的影响

为评估冷面绝热边界对试验结果的影响,在充分验证测控系统可控性和试验方法稳定性的基础上,分别以柔性隔热毡、刚性隔热瓦和纳米隔热材料为冷面绝热边界,对陶瓷纤维刚性隔热材料进行500℃、3 000s的石英灯热辐射考核试验,并利用迭代绝热边界当量热导率的方法对试验结果进行了模拟计算和分析。结果表明,以柔性隔热毡为冷面绝热边界时隔热材料的冷面温度最高,刚性隔热瓦和纳米隔热材料为冷面绝热边界时则冷面温度较低且相近,3者间最大相对偏差高达19.0%。模拟计算证实由冷面绝热边界材料引起的接触热阻对试验结果起到了决定性作用,而不简单地取决于绝热边界材料的隔热性能。     

Dynamic Modelling of the Hipparcos Attitude

We present a new method for a high-accuracy reconstruction of the attitude for a slowly spinning satellite. This method, referred to as the fully-dynamic approach, explores the possibility to describe the satellite's attitude as that of a rigid body subject to continuous external torques. The method is tried out on the Hipparcos data and is shown to reduce the noise for the along-scan attitude reconstruction for that mission by about a factor two to three. The dynamic modelling is expected to give a more accurate representation of the satellite's attitude than was obtained with a pure mathematical modelling. As such, it decreases the degrees of freedom in the a posteriori reconstruction. Some of the decrease is obtained through accumulating and subsequently implementing information on high frequency components in the solar radiation torques, which show to be systematic and predictable. This could be expected, as they are primarily linked to the external geometry and optical properties of the satellite. In the context of an astrometric mission, the methods presented here can only be applied as a final iteration step: the star positions that are used to reconstruct the attitude are also part of the scientific objectives of the mission. An estimate for the potential of a re-reduction of the Hipparcos data using the fully-dynamic model for the attitude reconstruction was obtained from test reductions of the first 24 months of mission data. Improvement of the accuracies of the astrometric parameters for all stars brighter than Hp=9.0 appears possible. The noise on the astrometric parameters for these stars was affected significantly by the along-scan attitude noise, which dominated for stars brighter than Hp=4.5. The possible improvement for stars brighter than about Hp=4.5 may, after iterations, be as much as a factor three. The reduced noise levels also allow a more accurate calibration and monitoring of instrument parameters, leading potentially to a better understanding of the instrument and the scientific data obtained with it. This revised version was published online in August 2006 with corrections to the Cover Date.