Microbial life in a liquid asphalt desert

Pitch Lake in Trinidad and Tobago is a natural asphalt reservoir nourished by pitch seepage, a form of petroleum that consists of mostly asphaltines, from the surrounding oil-rich region. During upward seepage, pitch mixes with mud and gases under high pressure, and the lighter portion evaporates or is volatilized, which produces a liquid asphalt residue characterized by low water activity, recalcitrant carbon substrates, and noxious chemical compounds. An active microbial community of archaea and bacteria, many of them novel strains (particularly from the new Tar ARC groups), totaling a biomass of up to 10(7) cells per gram, was found to inhabit the liquid hydrocarbon matrix of Pitch Lake. Geochemical and molecular taxonomic approaches revealed diverse, novel, and deeply branching microbial lineages with the potential to mediate anaerobic hydrocarbon degradation processes in different parts of the asphalt column. In addition, we found markers for archaeal methane metabolism and specific gene sequences affiliated with facultative and obligate anaerobic sulfur- and nitrite-oxidizing bacteria. The microbial diversity at Pitch Lake was found to be unique when compared to microbial communities analyzed at other hydrocarbon-rich environments, which included Rancho Le Brea, a natural asphalt environment in California, USA, and an oil well and a mud volcano in Trinidad and Tobago, among other sites. These results open a window into the microbial ecology and biogeochemistry of recalcitrant hydrocarbon matrices and establish the site as a terrestrial analogue for modeling the biotic potential of hydrocarbon lakes such as those found on Saturn's largest moon Titan.     

Radar Signal Propagation and Detection Through Ice

In this paper we describe the existing and planned radar measurements of the planetary bodies. The dielectric properties of water ice and other potential surface and subsurface materials are discussed, as well as their dependency on temperature and structure. We then evaluate the performance of subsurface sounding radars using these parameters. Finally we describe some laboratory technique to help interpret the radar data, presenting some results obtained using dielectric spectroscopy methods.     

Efficacy of an ototoxic aminoglycoside (gentamicin) on the differentiation of the inner ear of cichlid fish.

Previous investigations revealed that the growth of fish inner ear otoliths depends on the amplitude and the direction of gravity, thus suggesting the existence of a (negative) feedback mechanism. In the course of these experiments, it was shown that altered gravity both affected otolith size (and thus the provision of the proteinacious matrix) as well as the incorporation of calcium. It is hitherto unknown, as of whether sensory hair cells are involved either in the regulation of otolith growth or in the provision of otolithic material (such as protein or inorganic components) or even both. The ototoxic aminoglycoside gentamicin (GM) damages hair cells in many vertebrates (and is therefore used for the treatment of Meniere's disease in humans). The present study was thus designed to determine as of whether vestibular sensory cells are needed for otolith growth by applying GM in order to induce a (functionally relevant) loss of these cells. Developing cichlid fish Oreochromis mossambicus were therefore immersed in 120 mg/l GM for 10 or 21 days. At the beginning and at the end of the experimental periods, the fish were incubated in the calcium-tracer alizarin complexone (AC). After the experiment, otoliths were dissected and the area grown during GM-exposure (i.e., the area enclosed by the two AC labellings) was determined planimetrically. The results showed that incubating the animals in a GM-solution had no effect on otolith growth, but the development of otolith asymmetry was affected. Ultrastructural examinations of the sensory hair cells revealed that they had obviously not been affected by GM-treatment (no degenerative morphological features observed). Overall, the present results suggest that hair cells are not affected by GM concerning their possible role in (general) otolith growth, but that these cells indeed might have transitionally been impaired by GM resulting in a decreased capacity of regulating otolith symmetry.     

LOCSS and its implementation on the basis of a small satellite cluster

A problem of providing users with the necessary remote sensing data in the visible and near IR spectral bands has been considered. The solution of the problem plans increase of spatial and spectral resolution for imaging from space, high periodicity of surveying the same sites on the Earth's surface and spaceborne data delivery to users in real time.

This problem solution proposed is to use a cluster of small satellites and to implement the Local Space Service (LOCSS) program. The main aspects of this concept are as follows:

• • optimization of remote sensing instrumentation parameters;

• • image data compression onboard a small spacecraft;

• • compressed data downlinking via the low rate radio channel;

• • direct reception of the image data by users at small cheap receiving stations; and

• • image data decompression and processing using personal computers and special processors.

     

EUV coronal pattern of complexes of solar activity

Studying of the coronal plasma associated with long-lived complexes of the solar activity is important for understanding a relationship between the magnetic activity and the solar corona changing during the solar cycle.

In the present paper, two long-lived complexes of the solar activity at the beginning of the current solar cycle 23 are investigated by using the Extreme-Ultraviolet data (EUV) from SOHO/EIT. For this purpose the EIT limb synoptic maps during the CR1916–CR1919 (11 November 1996–1 March 1997) are obtained.

The coronal temperature structures derived from the three lines 171A (Fe IX,X), 195A (Fe XII)and 284A (Fe XV) are investigated by applying an algorithm developed by Zhang et al. [Zhang, J., White, S.M., Kundu, M.R. ApJ 527, 977, 1999]. Standard EIT software are used for the temperature estimation from the ratio of two lines of Fe IX,X and Fe XII.

The method of the rotational tomography with a correction for an inclination of the Earth’s orbit (B-angle) to the helioequator is applied to obtain the three-dimensional (3-D) coronal structure of the complex of the solar activity. The results reveal difference in temperature structures related to multi-poles magnetic structures of the complex of solar activity and to the typical, the bipolar activity complex.     

Monte Carlo calibration of the response of the University of Chicago’s Cosmic Ray Nuclei Experiment (CRNE) on IMP-8 to electrons above 0.5 MeV

Modern instrument-simulation techniques offer the possibility of increasing the scientific yield from archival space datasets. In this paper, we report on a simulation of the electron response of the University of Chicago’s Cosmic Ray Nuclei Experiment (CRNE) instrument on the IMP-8 satellite. IMP-8/CRNE returned data from 1973 to 2006. The CRNE particle telescope was designed to measure the isotopic composition of Galactic cosmic-ray (GCR) nuclei and has also been used in many studies of protons and ions above 10 MeV/nucleon from solar energetic particle (SEP) events. But CRNE also functions as a highly-capable detector for solar electrons above 0.5 MeV, an energy range that has not been extensively studied. Utilization of the CRNE electron data has heretofore been limited by the fact that CRNE was never calibrated for electrons. We have therefore used the GEANT4 Monte Carlo simulation package to model the CRNE response to electrons and (separately) protons for multiple energies and incident angles. The results were used to compute the energy- and angle-dependence of the effective area and the energy-dependence of the geometric factor. The response to protons, which was already well understood, was used to verify the mass model, the simulation settings, and the post-processing software. Our simulation of the IMP-8/CRNE electron response now allows analysis of hundreds of relativistic solar electron events observed by CRNE over the years, including studies of evolution of electron energy spectra with high time resolution. We show examples of these results and briefly discuss potential applications to future scientific investigations.     

Studies on precellular evolution: the encapsulation of polyribonucleotides by liposomes.

Liposomes are 5 to 50 micron vesicles with an internal aqueous environment, whose amphiphilic lipidic components self-assemble into systems with at least one double-layered membrane. Liposomes have been suggested as possible models of precellular systems formed in the early Archean Earth from lipids of non-enzymatic origin. Since it is generally accepted that RNA molecules preceded double-stranded DNA molecules as genetic material, we have studied the encapsulation of polyribonucleotides within liposomes made from dipalmitoyl phosphatidylcholine, and from egg yolk phosphatidylcholine to which cholesterol was added in some cases. The liposomes were prepared under anoxic conditions following the reverse phase evaporation method described by Szoka and Papahadjopoulos. Quantitative determinations show that approximately 50% of the available lipids form liposomes, and that up to 5% of the polyribonucleotides can be entrapped by them. We have also studied the encapsulation of polyribonucleotides in the presence of 1) urea and cyanamide, two non-electrolytes that have been used as prebiotic condensing agents, and 2) of Zn++ and Pb++, two cations employed in the non-enzymatic template-directed synthesis of polyribonucleotides from activated nucleotides.     

Perception of gravity by plants.

Physical principles can be used to predict some features about the gravity perception system in plants. The nature of the system has made it rather elusive, so this approach represents an additional source of information to help find it. For a gravitational stimulus to be detected, two masses must move relative to each other in a manner which causes a significant amount of work to be done on a receptor. Relative to cellular dimensions, the masses must be large, be dense and move noticeable distances. The main sources of noise are thermal motion and flexing of the plant tissue. Some new models for the function of amyloplasts as statoliths are presented.     

Iron meteorites can support the growth of acidophilic chemolithoautotrophic microorganisms

Chemolithoautotrophy based on reduced inorganic minerals is considered a primitive energy transduction system. Evidence that a high number of meteorites crashed into the planet during the early period of Earth history led us to test the ability of iron-oxidizing bacteria to grow using iron meteorites as their source of energy. Here we report the growth of two acidophilic iron-oxidizing bacteria, Leptospirillum ferrooxidans and Acidithiobacillus ferrooxidans, on a piece of the Toluca meteorite as the only source of energy. The alteration of the surface of the exposed piece of meteorite, the solubilization of its oxidized metal constituents, mainly ferric iron, and the formation of goethite precipitates all clearly indicate that iron-meteorite-based chemolithotrophic metabolism is viable.     

The structure of resting bacterial populations in soil and subsoil permafrost

The structure of individual cells in microbial populations in situ of the Arctic and Antarctic permafrost was studied by scanning and transmission electron microscopy methods and compared with that of cyst-like resting forms generated under special conditions by the non-spore-forming bacteria Arthrobacter and Micrococcus isolated from the permafrost. Electron microscopy examination of microorganisms in situ revealed several types of bacterial cells having no signs of damage, including "dwarf" curved forms similar to nanoforms. Intact bacterial cells in situ and frozen cultures of the permafrost isolates differed from vegetative cells by thickened cell walls, the altered structure of cytoplasm, and the compact nucleoid, and were similar in these features to cyst-like resting forms of non-spore-forming "permafrost" bacterial strains of Arthrobacter and Micrococcus spp. Cyst-like cells, being resistant to adverse external factors, are regarded as being responsible for survival of the non-spore-formers under prolonged exposure to subzero temperatures and can be a target to search for living microorganisms in natural environments both on the Earth and on extraterrestrial bodies.