Neutral buoyancy and sleep-deprived serum factors alter expression of cytokines regulating osteogenesis

We examined expression of genes associated with cytokine production, and genes implicated in regulating bone metabolism, in bone stromal and osteoblast cells incubated under standard ground conditions and under conditions of neutral buoyancy, and in the presence/absence of serum from normal or sleep-deprived mice. We observed a clear interaction between these two conditions (exposure to neutral buoyancy and serum stimulation) in promoting enhanced osteoclastogenesis. Both conditions independently altered expression of a number of cytokines implicated in the regulation of bone metabolism. However, using stromal cells from IL-1 and TNFx cytokine(r) KO mice, we concluded that the increased bone loss under microgravity conditions was not primarily cytokine mediated.     

The August 1972 solar-terrestrial events: Interplanetary magnetic field observations

A review is presented of the interplanetary magnetic field observations acquired in early August 1972 when four solar flares erupted in McMath Plage region 11976. Measurements of the interplanetary field were obtained by Earth satellites, HEOS-2 and Explorer 41, and by Pioneers 9 and 10 which, by good fortune, were radially aligned and only 45° east of the Earth-Sun direction. In response to the four flares, four interplanetary shocks were seen at Earth and at Pioneer 9, which was then at a heliocentric distance of 0.78 AU. However, at Pioneer 10, which was 2.2 AU from the Sun, only two forward shocks and one reverse shock were seen. The available magnetic field data acquired in the vicinity of the shocks are presented. Efforts to identify corresponding shocks at the several locations and to deduce their velocities of propagation between 0.8 and 2.2 AU are reviewed. The early studies were based on average velocities between the Sun and Pioneer 9, the Sun and Earth and the Sun and Pioneer 10. A large deceleration of the shocks between the Sun and 0.8 AU as well as between 0.8 and 2.2 AU was inferred. More recently the local velocities of the shocks at Pioneers 9 and 10 have become available. A comparison of these velocities shows little, if any, deceleration between 0.8 and 2.2 AU and implies that most or all of the deceleration actually occurred nearer the Sun. Evidence is also presented that shows a significant departure of the flare-generated shock fronts from spherical symmetry.     

Reduced lymphocyte activation in space: role of cell-substratum interactions.

We investigated the effect of substratum adhesiveness on stimulated lymphocyte blastogenesis by reducing and blocking cell adhesion with poly (2-hydroxyethyl methacrylate) (poly-HEMA) in a simple on-ground system. Cells grown on medium-thick and thick poly-HEMA films were rounded in shape and displayed no signs of spreading. By contrast, on tissue culture plastic and very thin poly-HEMA films, they showed clear signs of spreading. The mitogenic response of lymphocytes grown on thick poly-HEMA films was reduced by up to 68% of the control (tissue culture plastic). Interferon-gamma production was near zero when the cells were grown on the least adhesive substratum. On uncoated plastic, activated lymphocytes subjected to high gravity (20g) exhibited an increased proliferation rate (40%) compared with 1g. By contrast, on poly-HEMA, high gravity did not improve lymphocyte responsiveness. These results show that activated lymphocytes need to anchor and spread prior to achieving an optimal proliferation response. We conclude that decreased lymphocyte adhesion could contribute to the depressed in vitro lymphocyte responsiveness found in the microgravity conditions of space flight.     

Adaptive Input Filter Compensation for Switching Regulators

An input filter is frequently employed between a switching regulator and its power source. However, its presence often results in degradation of dynamic performances and stability. The detrimental interaction is between an input filter and a switching regulator and is a function of the input filter parameters and also of the supply voltage. An earlier paper presented an analysis and design procedure aimed at developing a feed-forward loop to cancel this undesirable interaction. The feed-forward design is extended here to encompass a scheme that automatically accounts for changes in the supply voltage; the result is an adaptive compensation that tracks the input voltage variations. Experimental results are presented that confirm the adaptive nature of the design.     

In space, CDs play a new tune

The development of a prototype instrument for biological tests aboard the International Space Station is reviewed. The instrument will employ specially formatted glass and plastic compact disks to conduct tests on biological samples. Special dyes will indicate the presence of proteins, DNA, and other chemicals. One benefit will be the ability to increase the number of trials in an experiment, lending greater validity to the results.     

Jets Downstream of Collisionless Shocks

Space Science Reviews - Particles are accelerated to very high, non-thermal energies in solar and space plasma environments. While energy spectra of accelerated electrons often exhibit a power law,...     

Comprehensive data reduction package for the Immersion GRating INfrared Spectrograph: IGRINS

We present a Python-based data reduction pipeline package (PLP) for the Immersion GRating INfrared Spectrograph (IGRINS), an instrument that covers the complete H- and K-bands in one exposure with a spectral resolving power of 40,000. The reduction steps carried out by the PLP include flat-fielding, background removal, order extraction, distortion correction, wavelength calibration, and telluric correction using spectra of A type standard stars. As the spectrograph has no moving parts, the PLP automatically reduces the data using predefined functions for the processes of order extraction, distortion correction, and wavelength calibration. Before the telluric correction of the target spectra, the intrinsic hydrogen absorption features of the standard A star are removed with a Gaussian fitting algorithm. The final result is the flux of the target as a function of wavelength. Users can customize the predefined functions for the extraction of the spectrum from the echellogram and adjust the parameters for the fitting functions for the spectra of celestial objects, using “fine-tuning” options, as necessary. Presently, the PLP produces the best results for point-source targets.