Application of GFP technique for cytoskeleton visualization onboard the International Space Station

Cytoskeleton recently attracted wide attention of cell and molecular biologists due to its crucial role in gravity sensing and trunsduction. Most of cytoskeletal research is conducted by the means of immunohistochemical reactions, different modifications of which are beneficial for the ground-based experiments. But for the performance onboard the space vehicles, they represent quite complicated technique which requires time and special skills for astronauts. In addition, immunocytochemistry provides only static images of the cytoskeleton arrangement in fixed cells while its localization in living cells is needed for the better understanding of cytoskeletal function. In this connection, we propose a new approach for cytoskeletal visualization onboard the ISS, namely, application of green fluorescent protein (GFP) from Aequorea victoria, which has the unique properties as a marker for protein localization in vivo. The creation of chimerical protein-GFP gene constructs, obtaining the transformed plant cells possessed protein-GFP in their cytoskeletal composition will allow receiving a simple and efficient model for screening of the cytoskeleton functional status in microgravity.     

The OSIRIS-REx Thermal Emission Spectrometer (OTES) Instrument

The OSIRIS-REx Thermal Emission Spectrometer (OTES) will provide remote measurements of mineralogy and thermophysical properties of Bennu to map its surface, help select the OSIRIS-REx sampling site, and investigate the Yarkovsky effect. OTES is a Fourier Transform spectrometer covering the spectral range 5.71–100 μm (\(1750\mbox{--}100~\mbox{cm}^{-1}\)) with a spectral sample interval of \(8.66~\mbox{cm}^{-1}\) and a 6.5-mrad field of view. The OTES telescope is a 15.2-cm diameter Cassegrain telescope that feeds a flat-plate Michelson moving mirror mounted on a linear voice-coil motor assembly. A single uncooled deuterated l-alanine doped triglycine sulfate (DLATGS) pyroelectric detector is used to sample the interferogram every two seconds. Redundant ～0.855 μm laser diodes are used in a metrology interferometer to provide precise moving mirror control and IR sampling at 772 Hz. The beamsplitter is a 38-mm diameter, 1-mm thick chemical vapor deposited diamond with an antireflection microstructure to minimize surface reflection. An internal calibration cone blackbody target provides radiometric calibration. The radiometric precision in a single spectrum is \(\leq2.2 \times 10^{-8}~\mbox{W}\,\mbox{cm}^{-2}\,\mbox{sr} ^{-1}/\mbox{cm}^{-1}\) between 300 and \(1350~\mbox{cm}^{-1}\). The absolute integrated radiance error is \(<1\%\) for scene temperatures ranging from 150 to 380 K. The overall OTES envelope size is \(37.5 \times 28.9 \times 52.2~\mbox{cm}\), and the mass is 6.27 kg. The power consumption is 10.8 W average. OTES was developed by Arizona State University with Moog Broad Reach developing the electronics. OTES was integrated, tested, and radiometrically calibrated on the Arizona State University campus in Tempe, AZ.     

The NASA Performance Assessment Workstation: cognitive performance during head-down bed rest

The NASA Performance Assessment Workstation was used to assess cognitive performance changes in eight males subjected to seventeen days of 6 degrees head-down bed rest. PAWS uses six performance tasks to assess directed and divided attention, spatial, mathematical, and memory skills, and tracking ability. Subjective scales assess overall fatigue and mood state. Subjects completed training trials, practice trials, bed rest trials, and recovery trials. The last eight practice trials and all bed rest trials were performed with subjects lying face-down on a gurney. In general, there was no apparent cumulative effect of bed rest. Following a short period of performance stabilization, a slight but steady trend of performance improvement was observed across all trials. For most tasks, this trend of performance improvement was enhanced during recovery. No statistically significant differences in performance were observed when comparing bed rest with the control period. Additionally, fatigue scores showed little change across all periods.     

Solar particle event dose distributions: parameterization of dose-time profiles

Calculations of total dose and dose equivalent as a function of time since the start of the event are presented for four of the major solar particle events that occurred during the period from August to December 1989. Results are presented for exposures to the skin, ocular lens and bone marrow shielded by a nominal thickness of aluminum shielding, comparable to that provided by a spacesuit. The calculated curves of organ dose and dose equivalent versus time are parameterized using a Weibull functional form for the fitting equation. The fitting parameters are determined using least squares regression techniques. These results provide a useful starting point for the development of methods to predict the cumulative doses and times to reach various dose limits from a limited number of dose measurements early in a solar particle event.     

Plasma Jet Motion Across the Geomagnetic Field in the “North Star” Active Geophysical Experiment

The active geophysical rocket experiment North Star was carried out in the auroral ionosphere on January 22, 1999, at the Poker Flat Research Range (Alaska, USA) using the American research rocket Black Brant XII with explosive plasma generators on board. Separable modules with scientific equipment were located at distances of from 170 to 1595 m from the plasma source. The experiment continued the series of the Russian–American joint experiments started by the Fluxus experiment in 1997. Two injections of aluminum plasma across the magnetic field were conducted in the North Star experiment. They were different, since in the first injection a neutral gas cloud was formed in order to increase the plasma ionization due to the interaction of neutrals of the jet and cloud. The first and second injections were conducted at heights of 360 and 280 km, respectively. The measurements have shown that the charged particle density was two orders of magnitude higher in the experiment with the gas release. The magnetic field in the first injection was completely expelled by the dense plasma of the jet. The displacement of the magnetic field in the second injection was negligible. The plasma jet velocity in both injections decreased gradually due to its interaction with the geomagnetic field. One of the most interesting results of the experiment was the conservation of high plasma density during the propagation of the divergent jet to considerable distances. This fact can be explained by the action of the critical ionization velocity mechanism.     

Magnetic Cusp as a Source of Dust Swarms in the Near-Earth Space

Within the framework of the concept of steady-state flow of a flux of dust particles through the cusp channel of the Earth's magnetosphere, we develop the idea that the magnetic cusp is a generator of dust swarms in the near-Earth space. The analytical dependencies for the density (concentration) of dust particles in the cusp and near-Earth space region are obtained.     

Uncontrollable Rotational Motion of the Mir Orbital Station

The results of determining the rotational motion of the Mir orbital station are presented for four long segments of its unmanned uncontrolled flight in 1999–2000. The determination was carried out using the data of onboard measurements of the Earth's magnetic field intensity. These data, taken for a time interval of several hours, were jointly processed by the least squares method with the help of integration of the equations of station motion relative to its center of mass. As a result of this processing, the initial conditions of motion and the parameters of the mathematical model used were evaluated. The technique of processing is verified using the telemetry data on angular velocity of the station and its attitude parameters. Two types of motion were applied on the investigated segments. One of them (three segments) presents a rotation around the axis of the minimum moment of inertia. This axis executes small oscillations with respect to a normal to the orbit plane. Such a motion was used for the first time on domestic manned orbital complexes. The second type of motion begins with a biaxial rotation which, in a few weeks, goes over into a motion very similar to the rotation around the normal to the orbit plane, but around the axis of the maximum moment of inertia.     

The Mode of Gravitational Orientation for the International Space Station

The results of the determination of the uncontrolled attitude motion of the International Space Station during its unmanned flight in 1999 are presented. The data of onboard measurements of three components of the angular velocity are used for this determination. These data covering an interval of slightly less than one orbit were jointly processed by the least squares method, by integrating the equations of motion of the station relative to its center of mass. As a result of this processing, the initial conditions of the motion and the parameters of the mathematical model used were estimated. The actual motion of the station has been determined for 20 such intervals during April–November. Throughout these intervals, the station rotated about the axis of the minimum moment of inertia, the latter executing small oscillations relative to the local vertical. Such a mode, known as the mode of gravitational orientation of a rotating satellite or the mode of generalized gravitational orientation, was planned before the flight. The measurements were made to verify it. The quasistatic component of the microaccelerations aboard the station is estimated for this mode.