Paramecium--a model system for studying cellular graviperception.

Experiments under varied gravitational accelerations as well as in density-adjusted media showed that sensation of gravity in protists may be linked to the known principles of mechanosensation. Paramecium, a ciliate with clear graviresponses (gravitaxis and gravikinesis) is an ideal model system to prove this hypothesis since the ciliary activity and thus the swimming behaviour is controlled by the membrane potential. It has also been assumed that the cytoplasmic mass causes a distinct stimulation of the bipolarly distributed mechano-sensitive K+ and Ca2+ ion channels in the plasma membrane in dependence of the spatial orientation of the cell. In order to prove this hypothesis, different channel blockers are currently under investigation. Gadolinium did not inhibit gravitaxis in Paramecium, showing that it does not specifically block gravireceptors. Further studies concentrated on the question of whether second messengers are involved in the gravity signal transduction chain. Exposure to 5 g for up to 10 min led to a significant increase in cAMP.     

Implementation of hand geometry: an analysis of user perspectives and system performance

This discusses the implementation issues of installing a commercially available hand geometry system in the current infrastructure of Purdue University's Recreational Sports Center. In addition to a performance analysis of the system, a pre- and post-data collection survey was distributed to the 129 test subjects gathering information on perceptions of biometrics, in particular hand geometry, as well as participants' thoughts and feelings during their interaction with the hand geometry device. The results of the survey suggest that participants were accepting of hand geometry. Additionally, analyses of the participants' survey responses revealed that 93% liked using hand geometry, 98% thought it was easy to use, and 87% preferred it to the existing card-based system, while nobody thought the device invaded their personal privacy. System performance achieved a 3-try match rate of 99.02% (FRR 0.98%) when "gaming"/potential impostor attempts were removed from analysis. The failure to enroll rate was zero. Statistical analyses exposed a significant difference in the scores of attempts made by users with prior hand geometry usage, and subjects that could not straighten out their hand on the device. However, there were no statistical difference in the effects of rings/no rings, improper hand placements/proper hand placements, or gender on hand geometry score.     

High Dynamic GPS Receiver Using Maximum Likelihood Estimationand Frequency Tracking

A new high dynamic global positioning system (GPS) receiver ispresented and its performance characterized by analysis,simulation, and demonstration. The demonstration receiver is abreadboard model capable of tracking a single simulated satellitesignal in pseudorange and range rate. Pseudorange and range rateestimates are made once every 20 ms, using a maximum likelihoodestimator, and are tracked by means of a third-order fadingmemory filter in a feedback configuration. The receiver trackspseudorange with rms errors of under 1 m when subjected tosimulated 50 g, 40 g/s circular trajectories. The tracking thresholdis approximately 28 dB·Hz, which provides 12 dB margin relativethe the minimum specified signal strength, assuming 3.5 dB systemnoise figure and 0 dB antenna gain.     

Dose equivalent, absorbed dose and charge spectrum investigation in low Earth orbit.

Particle intensity, dose equivalent and absorbed dose have been measured on board the space shuttle Endeavour during STS-108 in December 2001 by Dublin Institute for Advanced Studies (DIAS). The dose estimates are based on very accurate measurements of recoils produced in CR-39 by cosmic ray primary and secondary protons and heavier nuclei and by secondary neutrons. The corresponding LET spectra were used to determine dose equivalent and absorbed dose values. Estimates of the total flux of Z > or = 2 nuclei have been undertaken and a preliminary charge spectrum was measured. Some comparisons are made with preliminary data obtained on STS-105 (ISS Expedition) and other missions using CR-39 detectors.     

GPS based onboard and onground orbit operations for small satellites

In extension to common applications such as groundtrack displays and antenna steering, the SGP4 orbit model is proposed for operational orbit determination in small satellite missions. SGP4 is an analytical orbit model for Low-Earth orbiting satellites that is widely used for the propagation of NORAD twoline elements. Twoline elements may hence be generated completely independent of NORAD. Their use as exclusive source of orbital information simplifies the operations concept and reduces mission costs through the extensive use of existing low-cost mission support software. Due to small computer resource requirements of 8–10kByte, the SGP4 model may also be applied for onboard orbit computations making use of e.g. a 80186 processor, thus ensuring full compatibility of ground-based and onboard operations. The proposed approach is particularly suited in combination with a space-borne GPS receiver, were the C/A-code navigation solutions are treated as measurements that are adjusted in a least-squares sense using the SGP4 model. As consequence, inherent drawbacks of the pure navigation solutions such as data gaps and scatter as well as limited velocity accuracy are avoided, while the operational navigation activities are kept at a minimum. The feasibility of the concept is illustrated based on real GPS navigation data from the TOPEX/Poseidon and the MIR space station with an inherent data quality of 50–100 m. It is shown that 3 hours of data within a 4 day period are sufficient to keep the position error within 4 km, that is considered sufficient for most applications.     

Possible mechanisms of plant cell wall changes at microgravity.

Space and clinostatic experiments revealed that changes of plant cell wall structure and its function depend on type of tissue and duration of influence. It was shown that clinostat conditions reproduce the part of weightlessness biological effects. It is established that various responses of wall structural-metabolic organization occur at microgravity: changes of cell walls ultrastructure and organelles structure; decrease of synthesis of primary plant cell wall; rearrangements of polysaccharides content. It is shown that mechanisms of plant cell wall changes at microgravity are connected with decrease of cellulose crystallization, activation of pectolytic enzymes and rearrangement of calcium balance of apoplast and cytoplasm.     

New instrumentation for optical measuring of oxygen in gas or dissolved in liquids.

The optical oxygen sensor is a novel device for the determination of oxygen in gases or dissolved in liquids. It is based on the measurement principle of fluorescence quenching, which is completely different from that of polarographic oxygen sensors (today the most widespread devices of oxygen detection). The new instrument offers features and advantages, which render it not only a realistic alternative, but, for specific applications, make it superior to existing electrochemical methods. The system is based on low-cost semiconductor devices (light-emitting diodes, photodiodes, low-cost analogue and digital components) and new LED-compatible oxygen-sensitive membranes. The flow cell of the instrument may be thermostatted and the sensor can be calibrated by a simple two-point calibration procedure. The optical oxygen sensor is particularly suitable for measuring dissolved oxygen in respirometry, since no oxygen is consumed by the device and the signal is independent of sample flowrate or stirring speed. Typical fields of application are monitoring of oxygen in ground and drinking water, in process control in bioreactors and in breath gas and blood gas analysis.     

Molecular alterations in tumorigenic human bronchial and breast epithelial cells induced by high LET radiation.

Carcinogenesis is a multi-stage process with sequence of genetic events governing the phenotypic expression of a series of transformation steps leading to the development of metastatic cancer. In the present study, immortalized human bronchial (BEP2D) and breast (MCF-10F) cells were irradiated with graded doses of either 150 keV/micrometer alpha particles or 1 GeV/nucleon 56Fe ions. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Cell fusion studies indicated that radiation-induced tumorigenic phenotype in BEP2D cells could be completely suppressed by fusion with non-tumorigenic BEP2D cells. The differential expressions of known genes between tumorigenic bronchial and breast cells induced by alpha particles and their respective control cultures were compared using cDNA expression array. Among the 11 genes identified to be differentially expressed in BEP2D cells, three (DCC, DNA-PK and p21(CIP1)) were shown to be consistently down-regulated by 2 to 4 fold in all the 5 tumor cell lines examined. In contrast, their expressions in the fusion cell lines were comparable to control BEP2D cells. Similarly, expression levels of a series of genes were found to be altered in a step-wise manner among tumorigenic MCF-10F cells. The results are highly suggestive that functional alterations of these genes may be causally related to the carcinogenic process.