Molecular alterations in tumorigenic human bronchial and breast epithelial cells induced by high LET radiation.

Carcinogenesis is a multi-stage process with sequence of genetic events governing the phenotypic expression of a series of transformation steps leading to the development of metastatic cancer. In the present study, immortalized human bronchial (BEP2D) and breast (MCF-10F) cells were irradiated with graded doses of either 150 keV/micrometer alpha particles or 1 GeV/nucleon 56Fe ions. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Cell fusion studies indicated that radiation-induced tumorigenic phenotype in BEP2D cells could be completely suppressed by fusion with non-tumorigenic BEP2D cells. The differential expressions of known genes between tumorigenic bronchial and breast cells induced by alpha particles and their respective control cultures were compared using cDNA expression array. Among the 11 genes identified to be differentially expressed in BEP2D cells, three (DCC, DNA-PK and p21(CIP1)) were shown to be consistently down-regulated by 2 to 4 fold in all the 5 tumor cell lines examined. In contrast, their expressions in the fusion cell lines were comparable to control BEP2D cells. Similarly, expression levels of a series of genes were found to be altered in a step-wise manner among tumorigenic MCF-10F cells. The results are highly suggestive that functional alterations of these genes may be causally related to the carcinogenic process.     

Robust Kalman filter design for predictive wind shear detection

Severe low-altitude wind shear is a threat to aviation safety. Newly developed airborne sensors measure the radial component of wind along a line directly in front of an aircraft. The authors use optimal estimation theory to define a detection algorithm to warn of hazardous wind shear from these sensors. To achieve robustness, a wind shear detection algorithm must distinguish threatening wind shear from less hazardous gustiness, despite variations in wind shear structure. Statistical analysis methods to refine wind shear detection algorithm robustness are presented. Computational methods predict the ability to warn of severe wind shear and avoid false warning. Comparative capability of the detection algorithm as a function of its design parameters is determined, identifying designs that provide robust detection of severe wind shear     

Chromosome instability of HPRT-mutant subclones induced by ionising radiation of various LET.

The induction of HPRT-mutations and survival of Chinese hamster cells (line B11ii-FAF28, clone 431) were studied after irradiation by 4He and 12C-ions of various LET (20-360 keV/micrometers), produced by the U-200 heavy ion accelerator. The RBE increases with LET up to the maximum at 100-200 keV/micrometers and then decreases. Cytogenetic analysis was performed on the HPRT-mutant subclones selected from unirradiated Chinese hamster V-79 cells and from HPRT-mutant subclones that arose after exposure to gamma-rays, 1 GeV protons and 14N-ions (LET-77 keV/micrometers), produced by the synchrophasotron and the U-400M heavy ion accelerator. Slow growing mutant subclones were observed. The cytogenetic properties of individual clones were highly heterogeneous and chromosome instability was observed in both spontaneous and radiation-induced mutants. Chromosome instability was highest among spontaneous mutants and decreased with increasing LET.     

Design of the TRIO system-on-chip for aerospace

Several design and testing aspects of the TRIO smart sensor data acquisition chip, developed by JHU/APL for NASA spacecraft applications are presented. TRIO includes a 10 bit self-corrected analog-to-digital converter (ADC), 16/32 analog inputs, a front end multiplexer with selectable aquisition time, a current source, memory, serial and parallel bus, and control logic. So far TRIO is used in many missions including Contour, Messenger, Stereo, Pluto, and the generic JPL X2000 spacecraft bus.     

Controlled solar sail transfers into the near-sun orbits

We present results of numerical simulations of trajectories of a space probe with a flat solar sail which moves from a circular Earth orbit to near-Sun regions. We consider the upper limit of the sail temperature as the basic restriction. We also examine the planar (ecliptic) solar sail transfer with gravity-assist flybys around planets.     

Perestroika and glasnost in the soviet space programme A personal view

For many years the world-famous Kettering Group of amateur satellite observers have confounded many more ‘official’ organization with their accucary in tracking Soviet space launches. In this article the group's founder, Geoffrey E. Perry, MBE, presents his personal perspective on current developments in the Soviet space programme. The USSR's increased openness about non-sensitive issues is to be welcomed, he concludes, but the Kettering Group has work to do yet.     

Analysis of radiation risk from alpha particle component of solar particle events.

The solar particle events (SPE) will contain a primary alpha particle component, representing a possible increase in the potential risk to astronauts during an SPE over the often studied proton component. We discuss the physical interactions of alpha particles important in describing the transport of these particles through spacecraft and body shielding. Models of light ion reactions are presented and their effects on energy and linear energy transfer (LET) spectra in shielding discussed. We present predictions of particle spectra, dose, and dose equivalent in organs of interest for SPE spectra typical of those occurring in recent solar cycles. The large events of solar cycle 19 are found to have substantial increase in biological risk from alpha particles, including a large increase in secondary neutron production from alpha particle breakup.     

Modeling stress strain state of thin shells with a degenerating domain

We consider a possibility to apply the method of analyzing the complex-shaped shells, proposed in [1], for determining the stress strain state of thin shells with a degenerating domain. The results of numerical calculations are presented.     

Next generation test support systems for the aircraft life cycle

The objective of this paper is to present the experience of EADS in using a common test support system for integration and qualification testing, aircraft ground equipment (AGE), in-service software maintenance facilities (ISMF) and ground crew training. EADS uses its test support system AIDASS (Advanced Integrated Data Acquisition and Stimulation System) in different phases of the aircraft life cycle. AIDASS is comprised of a real-time front-end system and a standard PC for the user interface. The EADS test support system is used in almost all phases of the aircraft life cycle, for instance, for integration and verification testing, as aircraft ground equipment, for in-service software maintenance and training of ground crews. This test support system is used for the software maintenance in the TORNADO program at different airforces in Europe. The airforces also share test setups with industry     

MEMS Electronically Steerable Antennas for Fire Control Radars

Large apertures are of great benefit to applications that are prime powered limited as is found on aerostat and other airborne platforms. Electronically scanned array antennas are often proposed for these applications. However, increasing the aperture area with conventional array technology is met with prohibitive cost, weight, and prime power increases because of the dense spacing of phase shifters and/or active T/R modules. This discusses the recent development of RF MEMS (Microelectromechanical System) switch technology and the use of these switches in a Radanttrade lens configuration for arrays of approximately 10 m² at X-band. A proof-of-concept 0.4 m² MEMS Electronically Steerable Antenna (ESA) containing 25,000 MEMS switches has been successfully designed, fabricated, and tested. The 0.4 m² MEMS ESA was then integrated with an AN/APG-67 radar system to form the MEMS Demonstration Radar System. The MEMS Demonstration Radar System successfully detected both airborne and ground moving targets during a series of extensive radar demonstrations. This is believed to be the first large scale employment of MEMS switches in a scanning antenna and radar system. The low-cost, lightweight, and low power technology demonstrated can enable weight and power constrained platforms with electronic steering.