Life sciences flight hardware development for the International Space Station.

During the construction phase of the International Space Station (ISS), early flight opportunities have been identified (including designated Utilization Flights, UF) on which early science experiments may be performed. The focus of NASA's and other agencies' biological studies on the early flight opportunities is cell and molecular biology; with UF-1 scheduled to fly in fall 2001, followed by flights 8A and UF-3. Specific hardware is being developed to verify design concepts, e.g., the Avian Development Facility for incubation of small eggs and the Biomass Production System for plant cultivation. Other hardware concepts will utilize those early research opportunities onboard the ISS, e.g., an Incubator for sample cultivation, the European Modular Cultivation System for research with small plant systems, an Insect Habitat for support of insect species. Following the first Utilization Flights, additional equipment will be transported to the ISS to expand research opportunities and capabilities, e.g., a Cell Culture Unit, the Advanced Animal Habitat for rodents, an Aquatic Facility to support small fish and aquatic specimens, a Plant Research Unit for plant cultivation, and a specialized Egg Incubator for developmental biology studies. Host systems (Figure 1A, B: see text), e.g., a 2.5 m Centrifuge Rotor (g-levels from 0.01-g to 2-g) for direct comparisons between g and selectable g levels, the Life Sciences Glovebox for contained manipulations, and Habitat Holding Racks (Figure 1B: see text) will provide electrical power, communication links, and cooling to the habitats. Habitats will provide food, water, light, air and waste management as well as humidity and temperature control for a variety of research organisms. Operators on Earth and the crew on the ISS will be able to send commands to the laboratory equipment to monitor and control the environmental and experimental parameters inside specific habitats. Common laboratory equipment such as microscopes, cryo freezers, radiation dosimeters, and mass measurement devices are also currently in design stages by NASA and the ISS international partners.     

Ground performance of air conditioning and water recycle system for a Space Plant Box.

Researchers from 5 Japanese universities have developed a plant growth facility (Space Plant Box) for seed to seed experiments under microgravity. The breadboard model of the Space Plant Box was fabricated by assembling subsystems developed for microgravity. The subsystems include air conditioning and water recycle system, air circulation system, water and nutrient delivery system, lighting system and plant monitoring system. The air conditioning and water recycle system is simply composed of a single heat exchanger, two fans and hydrophilic fibrous strings. The strings allow water movement from the cooler fin in the Cooling Box to root supporting materials in the Plant Growth Chamber driven by water potential deficit. Relative humidity in the Plant Growth Chamber can be changed over a wide range by controlling the ratio of latent heat exchange to sensible heat exchange on the cooling fin of the heat exchanger. The transpiration rate was successfully measured by circulating air inside the Plant Growth Chamber only. Most water was recycled and a small amount of water needed to be added from the outside. The simple, air conditioning and water recycle system for the Space Plant Box showed good performance through a barley (Hordeum vulgare L.) growth experiment.     

Imaging the solar corona in the EUV

The SOHO (SOlar and Heliospheric Observatory) satellite was launched on December 2nd 1995. After arriving at the Earth-Sun (L1) Lagrangian point on February 14th 1996, it began to continuously observe the Sun. As one of the instruments onboard SOHO, the EIT (Extreme ultraviolet Imaging Telescope) images the Sun's corona in 4 EUV wavelengths. The He II filter at 304 Å images the chromosphere and the base of the transition region at a temperature of 5 − 8 × 10⁴ K; the Fe IX–X filter at 171 Å images the corona at a temperature of 1.3 × 10⁶ K; the Fe XII filter at 195 Å images the quiet corona outside coronal holes at a temperature of 1.6 × 10⁶ K; and the Fe XV filter at 284 Å images active regions with a temperature of 2.0 × 10⁶ K. About 5000 images have been obtained up to the present. In this paper, we describe also some aspects of the telescope and the detector performance for application in the observations. Images and movies of all the wavelengths allow a look at different phenomena present in the Sun's corona, and in particular, magnetic field reconnection.     

Cometary atmosphere (gas and dust)

There is important progress now in the identifications and measurements of primary (parent) molecules in the inner coma of Comet Halley. H₂O, CO₂ and CO are definitely in the list, CH and some complicate organic molecules are suspected. Gas production rate for water vapor is Q_H2O 10³⁰ s⁻¹. The bulk of data doesn't contradict to the Whipple model of nucleus (with clathrate modification). Pronounced spatial structure of gaseous flow in the coma was observed, but in general measured properties of neutral gas in the coma of Comet Halley are not very different from predicted. Situation for dust is different. In situ dust measurements show that size spectrum and optical properties of particles in coma are substantively declining from predicted on the base of groundbased photometry. However there are discrepancies between Vega and Giotto dust counter data. Dust in the inner coma didn't prevent the succesful imaging of nucleus by TV on Vega 1 and 2.     

Microlesions: theory and reality.

Efforts to assess radiation risk in space have been complicated by the considerable unknowns regarding the biological effects of the heavy ion component (HZE particles) of the cosmic rays. The attention has focused primarily on the assignation of a quality factor (Q) which would take into account the greater effectiveness of heavy ions vis-a-vis other forms of ionizing radiation. If however, as the so-called "Microlesion Theory" allows, the passage of HZE particles through living tissue produces unique biological damage, the traditional use of Q becomes meaningless. Therefore, it is critical to determine if microlesions, in fact, do exist. While the concept does not necessarily require detectable morphological damage, "tunnel-lesions" or holes in ocular tissues have been cited as evidence of microlesions. These data, however, are open to reinterpretation. On-going light, scanning and transmission electron microscopic studies of the corneas, lenses and retinas of rat eyes exposed to 450 MeV/amu 56Fe ions thus far have not revealed tunnel-lesion damage. The morphological effects of the heavy ions have been found to be qualitatively similar to the changes following other kinds of ionizing radiation.     

The influence of radiation quality on the formation of DNA breaks.

We have aimed to present a comprehensive review of our understanding to date of the formation of DNA strand breaks induced by high LET radiation. We have discussed data obtained from DNA in solution as well as from the formation and "repair" of strand breaks in cell DNA. There is good agreement, qualitatively, between these two systems. Results were evaluated for two parameters: (1) effectivity per particle, the cross section (sigma) in micrometers 2/particle; and (2) the strand break induction frequency as number of breaks per Gy per unit DNA (bp or dalton). A series of biological effects curves (one for each Z-number) is obtained in effectivity versus LET plots. The relationships between induction frequencies of single-strand breaks, or double-strand breaks, or the residual "irrepairable" breaks and LET-values have been evaluated and discussed for a wide spectrum of heavy ions, both for DNA in solution and for DNA in the cell. For radiation induced total breaks in cell DNA, the RBE is less than one, while the RBE for the induction of DSBs can be greater than one in the 100-200 keV/micrometers range. The level of irrepairable strand breaks is highest in this same LET range and may reach 25 percent of the initial break yield. The data presented cover results obtained for helium to uranium particles, covering a particle incident energy range of about 2 to 900 MeV/u with a corresponding LET range of near 16 to 16000 keV/micrometers.     

Plasma effects of active ion beam injections in the ionosphere at rocket altitudes

Data from ARCS rocket ion beam injection experiments will be primarily discussed in this paper. There are three results from this series of active experiments that are of particular interest in space plasma physics. These are the transverse acceleration of ambient ions in the large beam volume, the scattering of beam ions near the release payload, and the possible acceleration of electrons very close to the plasma generator which produce intense high frequency waves. The ability of 100 ma ion beam injections into the upper E and F regions of the ionosphere to produce these phenomena appear to be related solely to the process by which the plasma release payload and the ion beam are neutralized. Since the electrons in the plasma release do not convect with the plasma ions, the neutralization of both the payload and beam must be accomplished by large field-aligned currents (milliamperes/square meter) which are very unstable to wave growth of various modes. Future work will concentrate on the wave production and wave-particle interactions that produce the plasma/energetic particle effects discussed in this paper and which have direct application to natural phenomena in the upper ionosphere and magnetosphere.     

Observation of fundamental magnetoplasma emissions excited in magnetosphere by modulated electron beams

There were several cases when modulated electron beam had been injected from APEX satellite into an otherwise unmodified ionospheric plasma. The beams were formed from 2 μs pulses repeated at a rate of 40kHz. Injections took place in the altitude range 400–1100km over Europe. The onboard receiver connected to the dipole antenna swept the frequency interval 1–10 MHz in 1 sec. Due to a relatively narrow receiver bandwidth (15 kHz), moderate frequency step (50 kHz), slow changes of f_n and f_c (local electron plasma and gyro frequencies) fine structure of excited emissions was detected. In many cases, a very prominent doublet could be convincingly identified as a (f_n,f_u=sqrt(f_n^*f_n+f_c^*f_c)) band, with f_n practically equal to the local plasma frequency in the unperturbed ionosphere. Determination of plasma frequency is of prime importance in analysis of complex structures composed from various harmonics f_n,f_c,f_u. Complexity is manifested as coalescing of various harmonics with maxima shifted from nominal frequencies or splitting them into components due to mixing of local and propagation effects. Despite the existence of strong emissions on frequencies characteristic for the unperturbed ionosphere, very strong broadcasting transmissions are frequently cut off, even when the beam is directed upward. Most typical spectra are discussed.     

On the levels of enzymatic substrate specificity: implications for the early evolution of metabolic pathways.

The most frequently invoked explanation for the origin of metabolic pathways is the retrograde evolution hypothesis. In contrast, according to the so-called "patchwork" theory, metabolism evolved by the recruitment of relatively inefficient small enzymes of broad specificity that could react with a wide range of chemically related substrates. In this paper it is argued that both sequence comparisons and experimental results on enzyme substrate specificity support the patchwork assembly theory. The available evidence supports previous suggestions that gene duplication events followed by a gradual neoDarwinian accumulation of mutations and other minute genetic changes lead to the narrowing and modification of enzyme function in at least some primordial metabolic pathways.     

Lunar habitat concept employing the space shuttle external tank

The space shuttle external tank, which consists of a liquid oxygen tank, an intertank structure, and a liquid hydrogen tank, is an expendable structure used for approximately 8.5 min during each launch. A concept for outfitting the liquid oxygen tank-intertank unit for a 12-person lunar habitat is described. The concept utilizes existing structures and openings for both man and equipment access without compromising the structural integrity of the tank. Living quarters, instrumentation, environmental control and life support, thermal control, and propulsion systems are installed at Space Station Freedom. The unmanned habitat is then transported to low lunar orbit and autonomously soft landed on the lunar surface. Design studies indicate that this concept is feasible by the year 2000 with concurrent development of a space transfer vehicle and manned cargo lander for crew changeover and resupply.