Comparative metagenomics of two microbial mats at cuatro ciénegas basin I: ancient lessons on how to cope with an environment under severe nutrient stress

Abstract The Cuatro Ciénegas Basin (CCB) is an oasis in the desert of Mexico characterized by low phosphorus availability and by its great diversity of microbial mats. We compared the metagenomes of two aquatic microbial mats from the CCB with different nutrient limitations. We observed that the red mat was P-limited and dominated by Pseudomonas, while the green mat was N-limited and had higher species richness, with Proteobacteria and Cyanobacteria as the most abundant phyla. From their gene content, we deduced that both mats were very metabolically diverse despite their use of different strategies to cope with their respective environments. The red mat was found to be mostly heterotrophic, while the green mat was more autotrophic. The red mat had a higher number of transporters in general, including transporters of cellobiose and osmoprotectants. We suggest that generalists with plastic genomes dominate the red mat, while specialists with minimal genomes dominate the green mat. Nutrient limitation was a common scenario on the early planet; despite this, biogeochemical cycles were performed, and as a result the planet changed. The metagenomes of microbial mats from the CCB show the different strategies a community can use to cope with oligotrophy and persist. Key Words: Microbial mats-Metagenomics-Metabolism. Astrobiology 12, 648-658.     

Understanding Galaxy Formation with ISO Deep Surveys

We present the results obtained through the various ISO extragalactic deep surveys. Although IRAS revealed the existence of galaxies forming stars at a rate of a few tens (LIRGs) or even hundreds (ULIRGs) solar masses in the local universe, ISO not only discovered that these galaxies were already in place at redshift one, but also that they are not the extreme objects that we once believed them to be. Instead they appear to play a dominant role in shaping present-day galaxies as reflected by their role in the cosmic history of star formation and in producing the cosmic infrared background detected by the COBE satellite in the far infrared to sub-millimeter range. Based on observations with ISO, an ESA project with instruments funded by ESA Member States (especially the PI countries: France, Germany, The Netherlands, and the United Kingdom), and with the participation of ISAS and NASA.     

The Magnetometer Instrument on MESSENGER

The Magnetometer (MAG) on the MErcury Surface, Space ENvironment, GEochemistry, and Ranging (MESSENGER) mission is a low-noise, tri-axial, fluxgate instrument with its sensor mounted on a 3.6-m-long boom. The boom was deployed on March 8, 2005. The primary MAG science objectives are to determine the structure of Mercury’s intrinsic magnetic field and infer its origin. Mariner 10 observations indicate a planetary moment in the range 170 to 350 nT R _M³ (where R _M is Mercury’s mean radius). The uncertainties in the dipole moment are associated with the Mariner 10 trajectory and variability of the measured field. By orbiting Mercury, MESSENGER will significantly improve the determination of dipole and higher-order moments. The latter are essential to understanding the thermal history of the planet. MAG has a coarse range, ±51,300 nT full scale (1.6-nT resolution), for pre-flight testing, and a fine range, ±1,530 nT full scale (0.047-nT resolution), for Mercury operation. A magnetic cleanliness program was followed to minimize variable and static spacecraft-generated fields at the sensor. Observations during and after boom deployment indicate that the fixed residual field is less than a few nT at the location of the sensor, and initial observations indicate that the variable field is below 0.05 nT at least above about 3 Hz. Analog signals from the three axes are low-pass filtered (10-Hz cutoff) and sampled simultaneously by three 20-bit analog-to-digital converters every 50 ms. To accommodate variable telemetry rates, MAG provides 11 output rates from 0.01 s⁻¹ to 20 s⁻¹. Continuous measurement of fluctuations is provided with a digital 1–10 Hz bandpass filter. This fluctuation level is used to trigger high-time-resolution sampling in eight-minute segments to record events of interest when continuous high-rate sampling is not possible. The MAG instrument will provide accurate characterization of the intrinsic planetary field, magnetospheric structure, and dynamics of Mercury’s solar wind interaction.     

Development of the Primary Sorption Pump for the SEIS Seismometer of the InSight Mission to Mars

We report on the development of a passive sorption pump, capable of maintaining high-vacuum conditions in the InSight seismometer throughout the duration of any extended mission. The adsorber material is a novel zeolite-loaded aerogel (ZLA) composite, which consists of fine zeolite particles homogeneously dispersed throughout a porous silica network. The outgassing species within the SEIS evacuated container were analyzed and the outgassing rate was estimated by different methods. The results were used to optimize the ZLA composition to adsorb the outgassing constituents, dominated by water, while minimizing the SEIS bakeout constraints. The InSight ZLA composite additionally facilitated substantial CO₂ adsorption capabilities for risk mitigation against external leaks in Mars atmosphere. To comply with the stringent particle requirements, the ZLA getters were packaged in sealed containers, open to the SEIS interior through \(1~\upmu\mbox{m}\)-size pore filters. Results from experimental validation and verification tests of the packaged getters are presented. The pressure forecast based on these data, corroborated by rudimentary in situ pressure measurements, infer SEIS operational pressures not exceeding \(10^{-5}~\mbox{mbar}\) throughout the mission.     

Solar Cycle Forecasting

Predicting the behavior of a solar cycle after it is well underway (2–3 years after minimum) can be done with a fair degree of skill using auto-regression and curve fitting techniques that don’t require any knowledge of the physics involved. Predicting the amplitude of a solar cycle near, or before, the time of solar cycle minimum can be done using precursors such as geomagnetic activity and polar fields that do have some connection to the physics but the connections are uncertain and the precursors provide less reliable forecasts. Predictions for the amplitude of cycle 24 using these precursor techniques give drastically different values. Recently, dynamo models have been used directly with assimilated data to predict the amplitude of sunspot cycle 24 but have also given significantly different predictions. While others have questioned both the predictability of the solar cycle and the ability of current dynamo models to provide predictions, it is clear that cycle 24 will help to discriminate between some opposing dynamo models.     

The Lunar Orbiter Laser Altimeter Investigation on the Lunar Reconnaissance Orbiter Mission

The Lunar Orbiter Laser Altimeter (LOLA) is an instrument on the payload of NASA’s Lunar Reconnaissance Orbiter spacecraft (LRO) (Chin et al., in Space Sci. Rev. 129:391–419, 2007). The instrument is designed to measure the shape of the Moon by measuring precisely the range from the spacecraft to the lunar surface, and incorporating precision orbit determination of LRO, referencing surface ranges to the Moon’s center of mass. LOLA has 5 beams and operates at 28 Hz, with a nominal accuracy of 10 cm. Its primary objective is to produce a global geodetic grid for the Moon to which all other observations can be precisely referenced.     

Spatially resolved genomic, stable isotopic, and lipid analyses of a modern freshwater microbialite from cuatro ciénegas, Mexico

Abstract Microbialites are biologically mediated carbonate deposits found in diverse environments worldwide. To explore the organisms and processes involved in microbialite formation, this study integrated genomic, lipid, and both organic and inorganic stable isotopic analyses to examine five discrete depth horizons spanning the surface 25?mm of a modern freshwater microbialite from Cuatro Ciénegas, Mexico. Distinct bacterial communities and geochemical signatures were observed in each microbialite layer. Photoautotrophic organisms accounted for approximately 65% of the sequences in the surface community and produced biomass with distinctive lipid biomarker and isotopic (δ(13)C) signatures. This photoautotrophic biomass was efficiently degraded in the deeper layers by heterotrophic organisms, primarily sulfate-reducing proteobacteria. Two spatially distinct zones of carbonate precipitation were observed within the microbialite, with the first zone corresponding to the phototroph-dominated portion of the microbialite and the second zone associated with the presence of sulfate-reducing heterotrophs. The coupling of photoautotrophic production, heterotrophic decomposition, and remineralization of organic matter led to the incorporation of a characteristic biogenic signature into the inorganic CaCO(3) matrix. Overall, spatially resolved multidisciplinary analyses of the microbialite enabled correlations to be made between the distribution of specific organisms, precipitation of carbonate, and preservation of unique lipid and isotopic geochemical signatures. These findings are critical for understanding the formation of modern microbialites and have implications for the interpretation of ancient microbialite records. Key Words: Microbial ecology-Microbe-mineral interactions-Microbial mats-Stromatolites-Genomics. Astrobiology 12, 685-698.