Archaeal genome organization and stress responses: implications for the origin and evolution of cellular life

For DNA to be used as an informational molecule it must exist in the cell on the edge of stability because all genomic processes require local controlled melting. This presents mechanistic opportunities and problems for genomic DNA from hyperthermophilic organisms, whose unpackaged DNA could melt at optimal temperatures for growth. Hyperthermophiles are suggested to employ the novel positively supercoiling topoisomerase enzyme reverse gyrase (RG) to form positively supercoiled DNA that is intrinsically resistant to thermal denaturation. RG is presently the only archaeal gene that is uniquely found in hyperthermophiles and therefore is central to hypotheses suggesting a hypothermophilic origin of life. However, the suggestion that RG has evolved by the fusion of two pre-existing enzymes has led to hypotheses for a lower temperature for the origin of life. In addition to the action of topoisomerases, DNA packaging and the intracellular ionic environment can also manipulate DNA topology significantly. In the Euryarchaeota, nucleosomes containing minimal histones can adopt two alternate DNA topologies in a salt-dependent manner. From this we hypothesize that since internal salt concentrations are increased following an increase in temperature, the genomic effects of temperature fluctuations could also be accommodated by changes in nucleosome organization. In addition, stress-induced changes in the nucleoid proteins could also play a role in maintaining the genome in the optimal topological state in changing environments. The function of these systems could therefore be central to temperature adaptation and thus be implicated in origin of life scenarios involving hyperthermophiles.     

A search for optical beacons: implications of null results

Over the past few years a series of searches for interstellar radio beacons have taken place using the Parkes radio telescope. Here we report hitherto unpublished results from a search for optical beacons from 60 solar-type stars using the Perth-Lowell telescope. We discuss the significance of the null results from these searches, all of which were based on the interstellar contact channel hypothesis. While the null results of all searches to date can be explained simply by the nonexistence of electromagnetically communicating life elsewhere in the Milky Way, four other possible explanations that do not preclude its existence are proposed: (1) Extraterrestrial civilizations desiring to make contact through the use of electromagnetic beacons have a very low density in the Milky Way. (2) The interstellar contact channel hypothesis is incorrect, and beacons exist at frequencies that have not yet been searched. (3) The search has been incomplete in terms of sensitivity and/or target directions: Beacons exist, but more sensitive equipment and/or more searching is needed to achieve success. (4) The search has occurred before beacon signals can be expected to have arrived at the Earth, and beacon signals may be expected in the future. Based on consideration of the technology required for extraterrestrial civilizations to identify target planets, we argue that the fourth possibility is likely to be valid and that powerful, easily detectable beacons could be received in coming centuries.     

Eye light flashes on the Mir space station

The phenomenon of light flashes (LF) in eyes for people in space has been investigated onboard Mir. Data on particles hitting the eye have been collected with the SilEye detectors, and correlated with human observations. It is found that a nucleus in the radiation environment of Mir has roughly a 1% probability to cause an LF, whereas the proton probability is almost three orders of magnitude less. As a function of LET, the LF probability increases above 10 keV/micrometer, reaching about 5% at around 50 keV/micrometer.     

Nonlinear Localized Electrostatic Structures and Their Experimental Diagnostics in the Magnetosphere Plasma

The theoretical models of the formation of the three-dimensional quasi-stationary structures of variations of density and electrostatic potential in a multicomponent magnetosphere plasma are considered. On the basis of the perturbation method, we have studied the domains of the parametric space, where the occurrence of nonlinear quasi-stationary ion-acoustic and electron-acoustic structures are possible. For these structures, the velocities of motion along the direction of the magnetic field are estimated, together with the longitudinal and transverse scales with respect to the direction of the Earth's magnetic field. The calculated dependences of the scales l and l _|| of the structures on the plasma parameters in the three-component plasma allow one to compare the results of the considered theoretical models with new experimental data of measuring the form of soliton structures onboard the FAST, POLAR, and GEOTAIL satellites.     

Cosmic Microwave Background Anisotropy Observations

This paper discusses the status of observations of Cosmic Microwave Background (CMB) anisotropies. The first detections of primary anisotropies in the CMB, achieved about 10 years ago, boosted a large number of ground-based and balloon-borne experiments that have delineated the CMB angular power spectrum up to spherical harmonic multipole l1000. A wealth of information on cosmological parameters is being revealed by these measurements. Very recently, the positions and amplitudes of the first and second peak in the power spectrum have been determined providing strong support to inflationary models with adiabatic primordial density perturbations. A total density equal to the critical value, and baryonic density consistent with Big Bang nucleosynthesis are the first results emerging from the current CMB data. Future experiments on ground (mainly interferometers), in balloons and from space (MAP and Planck missions) have the potential to constrain more than 10 cosmological parameters with high precision.     

Beam-Park-Experiment-1/2000 with TIRA

A monostatic 24-h debris observation campaign (BPE-1/2000) has been prepared and conducted using FGAN's TIRA L-Band system. Based on experiences from previous Beam-park experiments a similar largely automated data processing is applied on an extended range window of 300–2000km. More than 1500 detections are encountered, 471 of them are verified as being real objects in Low-Earth-Orbit (LEO). PROOF's observation forecasting of catalogued objects is evaluated against the observed objects, and the difficulties obtaining radar cross-sections (RCSs) and object sizes from Beam-park experiments are discussed. Sidelobe detections are identified by using background information like two-line element (TLE) sets and/or catalogued RCSs.In comparison with previous experiments, the statistics show similarities confirming the concept of Beam-park experiments for space debris observations, despite the snapshot character of 24-h experiments. The comparison with MASTER/PROOF'99 and ORDEM2000 leads to a reasonable agreement between models and observations.     

The image/poetry education and public outreach program

Education and public outreach are viewed by NASA as significant undertakings for all of its space missions. The IMAGE satellite is one of the first missions to explicitly include `E&PO in its original proposal to NASA in 1996. We will discuss what IMAGE has accomplished in this area to date, and what new activities it will conduct following a successful launch.     

Study of cosmic rays and light flashes on board Space Station MIR: the SilEye experiment.

The SilEye experiment aims to study the cause and processes related to the anomalous Light Flashes (LF) perceived by astronauts in orbit and their relation with Cosmic Rays. These observations will be also useful in the study of the long duration manned space flight environment. Two PC-driven silicon detector telescopes have been built and placed aboard Space Station MIR. SilEye-1 was launched in 1995 and provided particles track and LF information; the data gathered indicate a linear dependence of FLF(Hz) ( 4 2) 10(3) 5.3 1.7 10(4) Fpart(Hz) if South Atlantic Anomaly fluxes are not included. Even though higher statistic is required, this is an indication that heavy ion interactions with the eye are the main LF cause. To improve quality and quantity of measurements, a second apparatus, SilEye-2, was placed on MIR in 1997, and started work from August 1998. This instrument provides energetic information, which allows nuclear identification in selected energy ranges; we present preliminary measurements of the radiation field inside MIR performed with SilEye-2 detector in June 1998.     

Halo White Dwarfs and Baryonic Dark Matter

We describe the formation of hot intergalactic gas along with baryonic remnants in galaxy halos. In this scenario, the mass and metallicity of the hot intracluster and intragroup gas relates directly to the production of baryonic remnants during the collapse of galactic halos. We construct a schematic but self-consistent model in which early bursts of star formation lead to a large remnant population in the halo, and to the outflow of stellar ejecta into the halo and ultimately the Local Group. We consider local as well as high redshift constraints on this scenario. This study suggests that the microlensing objects in the Galactic halo may predominantly be 0.5M white dwarfs, assuming that the initial mass function for early star formation favored the formation of intermediate mass stars with m 1M. However, the bulk of the baryonic dark matter in this scenario is associated with the ejecta of the white dwarf progenitors, and resides in the hot intergalactic medium.     

Cause and Effect At the Magnetopause

Recent analyses of spacecraft data, especially AMPTE/IRM data, provide a test of reconnection theory; an analysis for the signature of a local tangential stress balance in a one-dimensional time-stationary rotational discontinuity has left crucial questions unanswered. A key result is that the electron temperature profile inward through the magnetopause current sheet shows heating followed by cooling. Electrons must be one of the carriers of the current; hence this result reflects the sign of E · J in the frame of reference of the magnetopause current carriers. Since the current is directed from dawn to dusk, the inescapable conclusion is that the electric field must reverse within the current sheet. This is direct evidence of a load–dynamo combination; in that dynamo, energy is transferred from the solar wind plasma to the electromagnetic field. A dynamo is not included in the reconnection model which includes only the electrical load; therefore, we argue that the reconnection problem is improperly posed. A second compelling observation is a remarkable difference of the normal component of the plasma velocity between inbound and outbound crossings. For an inbound crossing (outward current meander) this component does reverse, but not quite as assumed in the reconnection model; on the other hand, for outbound crossings of the spacecraft (corresponding to erosion) there is no reversal at all. The normal component is approximately constant at 20 km s^-1, anti-Sunward throughout. Since the typical motion of the magnetopause is 10 km s^-1 this revealing result shows that solar wind plasma can go across the magnetopause, even onto closed field lines to feed the low latitude boundary layer. This is in stark contrast to the reconnection model where the plasma goes to open field lines. The interaction can be understood by appealing to Poynting's theorem, where E · J describes the net effect on or by the plasma. Time-dependent terms (even in the initial conditions) must be used so that it is possible to draw upon energy which has been stored locally in both electrical and magnetic forms. An extended discussion of observational results from ground-based, rocket, and satellite instruments indicate the impulsive nature of the solar wind–magnetospheric interaction. There is a lot of plasma involved in this interaction, over 10²⁷ ions electrons^-1 per second; the anti-Sunward flow takes place in the low latitude boundary layer. There is no flux catastrophe produced by this flow since the frozen-field theorem does not hold for plasma transfer across the magnetopause. The LLBL completely envelops the plasma sheet; the LLBL is the source of its plasma, not the plasma mantle as hypothesized in the reconnection model of the magnetotail. A number of serious errors have occurred in some articles in the literature on reconnection, and we list and discuss the most important of these. In the conclusion it is emphasized that the failure to provide a viable energy source, within the necessary spatial and temporal constraints, is responsible for the failure of reconnection model. This does not mean that the state of interconnection between the geomagnetic field and the interplanetary magnetic field can not change, but it does mean that the advocated process is not relevant to such changes. True reconnection requires that the electric field has a curl so that an electromotive force = E · dl = -d_Mdt exists through which energy can be interchanged with stored magnetic energy.