Plant molecular biology in the space station era: utilization of KSC fixation tubes with RNAlater

Spaceflight experiments involving biological specimens face unique challenges with regard to the on orbit harvest and preservation of material for later ground-based analyses. Preserving plant material for gene expression analyses requires that the tissue be prepared and stored in a manner that maintains the integrity of RNA. The liquid preservative RNAlater (Ambion) provides an effective alternative to conventional freezing strategies, which are limited or unavailable in current spaceflight experiment scenarios. The spaceflight use of RNAlater is enabled by the Kennedy space center fixation tube (KFT), hardware designed to provide the necessary containment of fixatives during the harvest and stowage of biological samples in space. Pairing RNAlater with the KFT system provides a safe and effective strategy for preserving plant material for subsequent molecular analyses, a strategy that has proven effective in several spaceflight experiments. Possible spaceflight scenarios for the use of RNAlater and KFTs are explored and discussed.     

Biosignatures from Earth-like planets around M dwarfs

Coupled one-dimensional photochemical-climate calculations have been performed for hypothetical Earth-like planets around M dwarfs. Visible/near-infrared and thermal-infrared synthetic spectra of these planets were generated to determine which biosignature gases might be observed by a future, space-based telescope. Our star sample included two observed active M dwarfs-AD Leo and GJ 643-and three quiescent model stars. The spectral distribution of these stars in the ultraviolet generates a different photochemistry on these planets. As a result, the biogenic gases CH4, N2O, and CH3Cl have substantially longer lifetimes and higher mixing ratios than on Earth, making them potentially observable by space-based telescopes. On the active M-star planets, an ozone layer similar to Earth's was developed that resulted in a spectroscopic signature comparable to the terrestrial one. The simultaneous detection of O2 (or O3) and a reduced gas in a planet's atmosphere has been suggested as strong evidence for life. Planets circling M stars may be good locations to search for such evidence.     

Some Technical Issues of an Optically Focused Small Space Debris Tracking and Cataloging System

Congressional language in the 1998 US Senate Armed Services Committee authorization bill directed ... the Secretary of the (United States) Air Force to undertake a design study of a system that could catalog and track debris down to one centimeter in size out to 1000kilometer in altitude. The US Air Force Research Laboratory, in conjunction with other US National Laboratories and the National Aeronautics and Space Administration (NASA) conducted a study that examined what technical systems and operations would be required to perform such a mission. This paper outlines the study process, details the findings, draws conclusions, and makes recommendations as to what would be needed to develop an optically based system capable of cataloging and tracking small debris in low Earth orbit.     

The NASA Astrobiology Roadmap

The NASA Astrobiology Roadmap provides guidance for research and technology development across the NASA enterprises that encompass the space, Earth, and biological sciences. The ongoing development of astrobiology roadmaps embodies the contributions of diverse scientists and technologists from government, universities, and private institutions. The Roadmap addresses three basic questions: how does life begin and evolve, does life exist elsewhere in the universe, and what is the future of life on Earth and beyond? Seven Science Goals outline the following key domains of investigation: understanding the nature and distribution of habitable environments in the universe, exploring for habitable environments and life in our own Solar System, understanding the emergence of life, determining how early life on Earth interacted and evolved with its changing environment, understanding the evolutionary mechanisms and environmental limits of life, determining the principles that will shape life in the future, and recognizing signatures of life on other worlds and on early Earth. For each of these goals, Science Objectives outline more specific high priority efforts for the next three to five years. These eighteen objectives are being integrated with NASA strategic planning.     

Modularity and Spacecraft Cost

From computers to aeronautics, many industries have achieved great cost savings through the use of modular engineering approaches. In standardizing interfaces between elements and by reusing functional units, projects can achieve direct reductions in required development labor and secondary savings from enhanced reliability via improved process control from manufacturing identical units. Issues involved in the extension of modular design to the space industry are discussed. A cost model is developed to resolve some of the conflicting advantages and disadvantages between modular and customized designs. Key regimes are identified that represent the best opportunities for applying modular concepts.     

Interplanetary transfer of photosynthesis: an experimental demonstration of a selective dispersal filter in planetary island biogeography

We launched a cryptoendolithic habitat, made of a gneissic impactite inoculated with Chroococcidiopsis sp., into Earth orbit. After orbiting the Earth for 16 days, the rock entered the Earth's atmosphere and was recovered in Kazakhstan. The heat of entry ablated and heated the rock to a temperature well above the upper temperature limit for life to below the depth at which light levels are insufficient for photosynthetic organisms ( approximately 5 mm), thus killing all of its photosynthetic inhabitants. This experiment shows that atmospheric transit acts as a strong biogeographical dispersal filter to the interplanetary transfer of photosynthesis. Following atmospheric entry we found that a transparent, glassy fusion crust had formed on the outside of the rock. Re-inoculated Chroococcidiopsis grew preferentially under the fusion crust in the relatively unaltered gneiss beneath. Organisms under the fusion grew approximately twice as fast as the organisms on the control rock. Thus, the biologically destructive effects of atmospheric transit can generate entirely novel and improved endolithic habitats for organisms on the destination planetary body that survive the dispersal filter. The experiment advances our understanding of how island biogeography works on the interplanetary scale.     

Cyanobacterial emergence at 2.8 gya and greenhouse feedbacks

Apparent cyanobacterial emergence at about 2.8 Gya coincides with the negative excursion in the organic carbon isotope record, which is the first strong evidence for the presence of atmospheric methane. The existence of weathering feedbacks in the carbonate-silicate cycle suggests that atmospheric and oceanic CO2 concentrations would have been high prior to the presence of a methane greenhouse (and thus the ocean would have had high bicarbonate concentrations). With the onset of a methane greenhouse, carbon dioxide concentrations would decrease. Bicarbonate has been proposed as the preferred reductant that preceded water for oxygenic photosynthesis in a bacterial photosynthetic precursor to cyanobacteria; with the drop of carbon dioxide level, Archean cyanobacteria emerged using water as a reductant instead of bicarbonate (Dismukes et al., 2001). Our thermodynamic calculations, with regard to this scenario, give at least a tenfold drop in aqueous CO2 levels with the onset of a methane-dominated greenhouse, assuming surface temperatures of about 60 degrees C and a drop in the level of atmospheric carbon dioxide from about 1 to 0.1 bars. The buildup of atmospheric methane could have been triggered by the boost in oceanic organic productivity that arose from the emergence of pre-cyanobacterial oxygenic phototrophy at about 2.8-3.0 Gya; high temperatures may have precluded an earlier emergence. A greenhouse transition timescale on the order of 50-100 million years is consistent with results from modeling the carbonate-silicate cycle. This is an alternative hypothesis to proposals of a tectonic driver for this apparent greenhouse transition.     

High-Energy Atmospheric Physics: Terrestrial Gamma-Ray Flashes and Related Phenomena

It is now well established that both thunderclouds and lightning routinely emit x-rays and gamma-rays. These emissions appear over wide timescales, ranging from sub-microsecond bursts of x-rays associated with lightning leaders, to sub-millisecond bursts of gamma-rays seen in space called terrestrial gamma-ray flashes, to minute long glows from thunderclouds seen on the ground and in or near the cloud by aircraft and balloons. In particular, terrestrial gamma-ray flashes (TGFs), which are thought to be emitted by thunderclouds, are so bright that they sometimes saturate detectors on spacecraft hundreds of kilometers away. These TGFs also generate energetic secondary electrons and positrons that are detected by spacecraft in the inner magnetosphere. It is generally believed that these x-ray and gamma-ray emissions are generated, via bremsstrahlung, by energetic runaway electrons that are accelerated by electric fields in the atmosphere. In this paper, we review this newly emerging field of High-Energy Atmospheric Physics, including the production of runaway electrons, the production and propagation of energetic radiation, and the effects of both on atmospheric electrodynamics.     

高超声速下表面凸起干扰气动热实验研究

 对高超声速飞行器表面凸起附近的气流流动和气动加热开展了实验研究和分析。实验在高超声速炮风洞中进行,来流马赫数为8.2、单位雷诺数为9.35×10⁶ m^-1。利用薄膜传热测量方法进行了凸起几何形状和边界层状态对干扰流动加热的影响评估。利用流油图谱和纹影摄像法得到了凸起周围的流动特征:若凸起上游边界层未分离,最大峰值热流发生在凸起侧方附近处;若凸起上游边界层完全分离,最大峰值热流通常发生在凸起的上游表面。实验发现最大峰值热流和来流边界层状态关系不大,原因是流动干扰区表现出较强的三维扰动特性,使得来流层流边界层在干扰区内会转变成过渡甚至完全湍流状态。