The simulated silicification of bacteria--new clues to the modes and timing of bacterial preservation and implications for the search for extraterrestrial microfossils

Evidence of microbial life on Earth has been found in siliceous rock formations throughout the geological and fossil record. To understand the mechanisms of silicification and thus improve our search patterns for evidence of fossil microbial life in rocks, a series of controlled laboratory experiments were designed to simulate the silicification of microorganisms. The bacterial strains Pseudomonas fluorescens and Desulphovibrio indonensis were exposed to silicifying media. The experiments were designed to determine how exposure time to silicifying solutions and to silicifying solutions of different Si concentration affect the fossilization of microbial biofilms. The silicified biofilms were analyzed using transmission electron microscopy (TEM) in combination with energy-dispersive spectroscopy. Both bacterial species showed evidence of silicification after 24 h in 1,000 ppm silica solution, although D. indonensis was less prone to silicification. The degree of silicification of individual cells of the same sample varied, though such variations decreased with increasing exposure time. High Si concentration resulted in better preservation of cellular detail; the Si concentration was more important than the duration in Si solution. Even though no evidence of amorphous silica precipitation was observed, bacterial cells became permineralized. High-resolution TEM analysis revealed nanometer-sized crystallites characterized by lattice fringe-spacings that match the (10-11) d-spacing of quartz formed within bacterial cell walls after 1 week in 5,000 ppm silica solution. The mechanisms of silicification under controlled laboratory conditions and the implication for silicification in natural environments are discussed, along with the relevance of our findings in the search for early life on Earth and extraterrestrial life.     

Molecular identification of cyanobacteria associated with stromatolites from distinct geographical locations

Modern stromatolites represent a significant resource for studying microbial ecology and evolution. A preliminary investigation was undertaken employing specific genetic probes to characterize the cyanobacteria responsible for stromatolite construction in a range of environments, including microbial mats found in Australia not previously examined with molecular methods. Isolates of cyanobacteria were collected from stromatolites in thermal springs, hypersaline lakes, and oceanic fringes on two continents. A polymerase chain reaction specific for DNA of cyanobacterial 16S rRNA was developed, the resulting products of the DNA amplification reaction were sequenced, and the data were used to infer relatedness between the isolates studied and other members of the cyanobacterial radiation. Complete sequence was generated for the region from position 27 to 408 for 13 strains of cyanobacteria associated with stromatolites. All stromatolite-derived sequences were most closely related to cyanobacteria, as indicated by local sequence alignment. It was possible to correlate genetic identity with morphological nomenclatures and to expand the phylogeny of benthic cyanobacteria. These inferences were also expanded to temporal variation in the dominant resident cyanobacterial species based on sampling of surface and core sinter laminations. Under the methods employed, only one cyanobacterial strain was detected in each sample, suggesting the possible dominance of a specific clonal population of cyanobacteria at any one time in the biota of the samples tested. The data indicate that internal core samples of a stromatolite at least 10 years old can be successfully analyzed by DNA-based methods to identify preserved cyanobacteria.     

The magnetosheath and magnetotail of Venus

We describe the observational history and assess the current understanding of the magnetosheath and magnetotail of Venus, stressing recent developments. We make recommendations for research that can be done using existing observations, as well as desirable trajectory and instrumentation characteristics for future spacecraft missions.     

Key science questions from the second conference on early Mars: geologic, hydrologic, and climatic evolution and the implications for life

In October 2004, more than 130 terrestrial and planetary scientists met in Jackson Hole, WY, to discuss early Mars. The first billion years of martian geologic history is of particular interest because it is a period during which the planet was most active, after which a less dynamic period ensued that extends to the present day. The early activity left a fascinating geological record, which we are only beginning to unravel through direct observation and modeling. In considering this time period, questions outnumber answers, and one of the purposes of the meeting was to gather some of the best experts in the field to consider the current state of knowledge, ascertain which questions remain to be addressed, and identify the most promising approaches to addressing those questions. The purpose of this report is to document that discussion. Throughout the planet's first billion years, planetary-scale processes-including differentiation, hydrodynamic escape, volcanism, large impacts, erosion, and sedimentation-rapidly modified the atmosphere and crust. How did these processes operate, and what were their rates and interdependencies? The early environment was also characterized by both abundant liquid water and plentiful sources of energy, two of the most important conditions considered necessary for the origin of life. Where and when did the most habitable environments occur? Did life actually occupy them, and if so, has life persisted on Mars to the present? Our understanding of early Mars is critical to understanding how the planet we see today came to be.     

Graviperception in ciliates: steps in the transduction chain.

Ciliates represent suitable model systems to study the mechanisms of graviperception and signal transduction as they show clear gravity-induced behavioural responses (gravitaxis and gravikinesis). The cytoplasm seems to act as a "statolith" stimulating mechanosensitive ion channels in the cell membrane. In order to test this hypothesis, electrophysiological studies with Stylonychia mytilus were performed, revealing the proposed changes (de- or hyperpolarization) depending on the cell's spatial orientation. The behaviour of Paramecium and Stylonychia was also analyzed during variable acceleration conditions of parabolic flights (5th German Parabolic Flight Campaign, 2003). The corresponding data confirm the relaxation of the graviresponses in microgravity as well as the existence of thresholds of graviresponses, which are found to be in the range of 0.4xg (gravikinesis) and 0.6xg (gravitaxis).     

A management approach for allocating instrument development resources

The Cassini Science Management Plan was developed for NASA's Cassini mission to Saturn to assist the Science Instrument Manager in guiding the development of the spacecraft's science payload. This plan, unlike previous development approaches, allocated the entire mass, power, data rate and budget resources for the science instruments to the Principal Investigators. The result removed the Cassini Project from adjudicating and reallocating resources for instrument development problems. Instrument development problems that did occur were resolved by the Principal Investigators themselves through the use of a ‘resource exchange’. The exchange allowed Principal Investigators to submit ‘bids’ (i.e. a request for resources) to a database. Principal Investigators were allowed to barter their own resources with other investigators. The resulting exchange or multilateral trade allowed the investigators to reallocate their resource to ‘better’ their current position.     

DNA structures and radiation injury.

In the present paper experimental results from radiobiological investigations of the sedimentation behaviour of damaged and restored DNA-subunits attached to the nuclear membrane have been summarized. The studies were carried out preferably with Chinese Hamster cells V79-4 irradiated with different kinds of radiation (gamma-rays, neutrons and carbon ions) using the nucleoid sedimentation technique. Single-strand breaks relax the supercoiled DNA in the subunits resulting in a decreased sedimentation velocity. Rejoining leads to a correct restoration of the structure as can be studied by means of postincubation irradiation. Double-strand breaks release DNA fragments, again leading to an increased sedimentation velocity. If the average number of the induced double-strand breaks per subunit increases to a number higher than one, the measured results suggest that the structures should not be restored completely. The results are compatible with a new repair model developed in our laboratory on the assumption that, firstly, the single DNA subunits are the sensitive target rather than the whole DNA and, secondly, the repair of DNA damage takes place independently in each subunit.     

The meteorite flux

Meteorites are being retrieved from the surface of our planet all the time, the number of located falls being at maximum in the range 0.3 to 1.0 yr^-1 (10⁶ km²)^-1. Using the known flux of meteorite parent bodies to the top of the atmosphere this paper reviews suggested ablation processes and predicts the actual flux of meteorites to the Earth. It is found that this is some 70 to 300 times what is observed.     

Missions to the asteroid anteros and the space of true anomalies

Three opportunities for missions to rendezvous ballistically with the Earth-crossing asteroid Anteros are studied to illustrate the requirements for a trip to a near-Earth minor planet. The rationale, sample payload, spacecraft requirements and trajectory characteristics of these opportunities are typical of a rendezvous mission to an accessible near-Earth object. Round trip ballistic trajectories to return small samples of the asteroid with launch dates between 1985 and 2000 are also presented. Contours of minimum total ΔV drawn in the space of launch and arrival true anomalies, given the designation Prime Rib curves, are introduced as a useful tool for mission design.     

Magnetospheric cosmic-ray cutoffs and their variations

Many observations of geomagnetic cutoff phenomena and their implications with respect to the dynamics of charged particles in the geomagnetic field are discussed. Störmer's analytic treatment of the motion of charged particles in a dipole field is briefly reviewed, as are the approximate treatments of charged particle motions, first developed by Alfvén, which were to find successful application to the more complex fields now known to exist in the magnetosphere. In conclusion, the present understanding of geomagnetic cutoffs, together with some remaining areas of uncertainty are examined.