Clinorotation reduces number, but not size, of cartilaginous nodules formed in micromass cultures of mouse limbbud cells.

In previous studies we used a ground based model to investigate the cellular responses to microgravity by exposing micromass cultures of embryonic limb cells to simulated weightlessness on a clinostat. Cultures set up in T-flasks and rotated at 30 rpm showed that clinostatted cultures had less chondrocyte differentiation than stationary or rotation controls, as assessed by number of nodules/culture stained with cartilage specific Alcian blue. In the current study, nodule size and shape of these nodules was assessed by interactive measurement of area, perimeter, circularity, and equivalent diameters, using the Optimas imaging software. Results show no significant difference in any of the measurements, indicating that clinorotation has no effect on expansion of the nodules either by differentiation of cells within the nodule, or by recruitment of cells into the nodule. The reduction in number of nodules without an alteration in size and shape indicates that the effect of simulated microgravity is to reduce the cell interactions required for the initial condensation of cells into a nodule, probably by interference with cell adhesion molecules.     

Tissue responses to low protracted doses of high LET radiations or photons: early and late damage relevant to radio-protective countermeasures.

Early and late murine tissue responses to single or fractionated low doses of heavy charged particles, fission-spectrum neutrons or gamma rays are considered. Damage to the hematopoietic system is emphasized, but results on acute lethality, host response to challenge with transplanted leukemia cells and life-shortening are presented. Low dose rates per fraction were used in some neutron experiments. Split-dose lethality studies (LD 50/30) with fission neutrons indicated greater accumulation of injury during a 9 fraction course (over 17 days) than was the case for gamma-radiation. When total doses of 96 or 247 cGy of neutrons or gamma rays were given as a single dose or in 9 fractions, a significant sparing effect on femur CFU-S depression was observed for both radiation qualities during the first 11 days, but there was not an earlier return to normal with dose fractionation. During the 9 fraction sequence, a significant sparing effect of low dose rate on CFU-S depression was observed in both neutron and gamma-irradiated mice. CFU-S content at the end of the fractionation sequence did not correlate with measured LD 50/30. Sustained depression of femur and spleen CFU-S and a significant thrombocytopenia were observed when a total neutron dose of 240 cGy was given in 72 fractions over 24 weeks at low dose rates. The temporal aspects of CFU-S repopulation were different after a single versus fractionated neutron doses. The sustained reduction in the size of the CFU-S population was accompanied by an increase in the fraction in DNA synthesis. The proliferation characteristics and effects of age were different for radial CFU-S population closely associated with bone, compared with the axial population that can be readily aspirated from the femur. In aged irradiated animals, the CFU-S proliferation/redistribution response to typhoid vaccine showed both an age and radiation effect. After high single doses of neutrons or gamma rays, a significant age- and radiation-related deficiency in host defense mechanisms was detected by a shorter mean survival time following challenge with transplantable leukemia cells. Comparison of dose-response curves for life shortening after irradiation with fission-spectrum neutrons or high energy silicon particles indicated high initial slopes for both radiation qualities at low doses, but for higher doses of silicon, the effect per Gy decreased to a value similar to that for gamma rays. The two component life-shortening curve for silicon particles has implications for the potential efficacy of radioprotectants. Recent studies on protection against early and late effects by aminothiols, prostaglandins, and other compounds are discussed.     

Dynamics of a Solenoidal-Torqued Gyro-Stabilized Seeker Assembly for Guidance and Tracking

Several low-cost gyro-stabilized, bang-bang seeker assemblies for missile guidance and tracking applications have used similar physical structures. A magnetic dipole is an integral part of the gyro rotor assembly. The nominal spin axis of the gyro is colinear with the axis of a cylinder about which is wound a helix of wire. By properly modulating the current that is passed through that helix, the precession torque acting upon the gyroscope is controlled. One rather generalized model of such a seeker assembly is modeled, the equations of motion are derived, and the dominant error sources are identified and parametrically evaluated. Because this is such a commonly encountered structure, yet no such analysis has been published, to the author's knowledge, it is hoped that this is a useful contribution to the literature.     

Molecular spectroscopy of planetary atmospheres

Summary Clearly spectroscopy has already provided considerable insight into the nature of the planetary atmospheres. Several molecular constituents have been positively identified and upper limits have been placed on the abundances of others which are of particular significance in devising planetary models. But, equally clearly, the inadequacy of the spectral data, due to low spectral and/or spatial resolution, renders deductions of abundances, temperatures and pressures very uncertain. With the sole exception of the Martian CO₂ abundance, knowledge of the concentrations of planetary atmospheric constituents is unsatisfactory, and all numbers given must be regarded as only crude estimates. This latter comment applies equally well to the derived temperatures and pressures.What is urgently needed is the development of a reasonably complete theory of line formation in planetary atmospheres. This, coupled with more detailed spectroscopic work, should produce numerical values of atmospheric parameters which can be used with assurance.This work was performed with the aid of NASA Grant NsG-101-61 with funds administered by the University of California.     

Propagation and interaction of interplanetary transient disturbances. Numerical simulations

We study the heliocentric evolution of ICME-like disturbances and their associated transient forward shocks (TFSs) propagating in the interplanetary (IP) medium comparing the solutions of a hydrodynamic (HD) and magnetohydrodynamic (MHD) models using the ZEUS-3D code [Stone, J.M., Norman, M.L., 1992. Zeus-2d: a radiation magnetohydrodynamics code for astrophysical flows in two space dimensions. i – the hydrodynamic algorithms and tests. Astrophysical Journal Supplement Series 80, 753–790]. The simulations show that when a fast ICME and its associated IP shock propagate in the inner heliosphere they have an initial phase of about quasi-constant propagation speed (small deceleration) followed, after a critical distance (deflection point), by an exponential deceleration. By combining white light coronograph and interplanetary scintillation (IPS) measurements of ICMEs propagating within 1 AU [Manoharan, P.K., 2005. Evolution of coronal mass ejections in the inner heliosphere: a study using white-light and scintillation images. Solar Physics 235 (1–2), 345–368], such a critical distance and deceleration has already been inferred observationally. In addition, we also address the interaction between two ICME-like disturbances: a fast ICME 2 overtaking a previously launched slower ICME 1. After interaction, the leading ICME 1 accelerates and the tracking ICME 2 decelerates and both ICMEs tend to arrive at 1 AU having similar speeds. The 2-D HD and MHD models show similar qualitative results for the evolution and interaction of these disturbances in the IP medium.     

Features of rotational motion of a spacecraft descending in the Martian atmosphere

Angular motion at atmospheric entry is studied in the paper for a spacecraft with a bi-harmonic moment characteristic. Special attention is given to the case when the spacecraft possesses two stable balanced positions, and, hence, it can oscillate in dense atmospheric layers in the ranges of small or large angles of attack. The averaged equations of spacecraft motion are derived, which allow one to increase the speed of calculations by several orders of magnitude. A real example is presented, which concerns a spacecraft specially designed for descending in the Martian atmosphere.     

Coronal radio-sounding detection of a CME during the 1997 Galileo solar conjunction

Frequency fluctuations of the Galileo S-band radio signal were recorded nearly continuously during the spacecraft’s solar conjunction from December 1996 to February 1997. A strong propagating disturbance, most probably associated with a coronal mass ejection (CME), was detected on 7 February when the radio ray path proximate point was on the west solar limb at about 54 solar radii from the Sun. The CME passage through the line of sight is characterized by a significant increase in the fluctuation intensity of the recorded frequency and by an increase in the plasma speed from about 234 km s⁻¹ up to about 755 km s⁻¹. These velocity estimates are obtained from a correlation analysis of frequency fluctuations recorded simultaneously at two widely-separated ground stations. The density turbulence power spectrum is found to steepen behind the CME front. The Galileo radio-sounding data are compared with SOHO/LASCO observations of the CME in the corona and with WIND spacecraft data near the Earth’s orbit.     

Identification Techniques for SARSAT Signals

A process for the identification of emergency locator transmitter (ELT) signals related to search and rescue satellite-aided tracking (SARSAT) is presented. The ELT identification process is particularly important in order to increase the probability of detection and eliminate sources of interference from the data set. A set of ELT signal parameters is introduced and methods for estimating these parameters are developed. A theoretical analysis and performance evaluation of these methods is provided.     

Instrumentation for investigation of the depth-dose distribution by the Liulin-5 instrument of a human phantom on the Russian segment of ISS for estimation of the radiation risk during long term space flights.

Described is the Liulin-5 experiment and instrumentation, developed for investigation of the space radiation doses depth distribution in a human phantom on the Russian Segment of the International Space Station (ISS). Liulin-5 experiment is a part of the international project MATROSHKA-R on ISS. The experiment MATROSHKA-R is aimed to study the depth dose distribution at the sites of critical organs of the human body, using models of human body-anthropomorphic and spherical tissue-equivalent phantoms. The aim of Liulin-5 experiment is long term (4-5 years) investigation of the radiation environment dynamics inside the spherical tissue-equivalent phantom, mounted in different places of the Russian Segment of ISS. Energy deposition spectra, linear energy transfer spectra, flux and dose rates for protons and the biologically-relevant heavy ion components of the galactic cosmic radiation will be measured simultaneously with near real time resolution at different depths of the phantom by a telescope of silicon detectors. Data obtained together with data from other active and passive dosimeters will be used to estimate the radiation risk to the crewmembers, verify the models of radiation environment in low Earth orbit, validate body transport model and correlate organ level dose to skin dose. Presented are the test results of the prototype unit. The spherical phantom will be flown on the ISS in 2004 year and Liulin-5 experiment is planned for 2005 year.