Chromosomal intrachanges induced by swift iron ions.

We measured the induction of structural aberrations in human chromosome 5 induced by iron ions using the novel technique of multicolor banding in situ hybridization (mBAND). Human lymphocytes isolated from whole blood were exposed in vitro to 500 MeV/n (LET=200 keV/micrometers, doses 1 or 4 Gy) Fe nuclei at the HIMAC accelerator in Chiba (Japan). Chromosomes were prematurely condensed by calyculin A after 48 h in culture and slides were painted by mBAND. We found a frequency of 0.11 and 0.57 residual breakpoints per chromosome 5 after 1 and 4 Gy Fe-ions, respectively. Inter-chromosomal exchanges were the prevalent aberration type measured at both doses, followed by terminal deletions, and by intra-chromosomal exchanges. Among intra-chromosomal exchanges, intra-arm events were more frequent than inter-arm, but a significant number of intra-changes was associated to inter-changes involving the same chromosome after 4 Gy of iron ions. These events show that the complexity of chromosomal exchanges induced by heavy ions can be higher than expected by previous FISH studies.     

Organization of cytoskeleton during differentiation of gravisensitive root sites under clinorotation.

Key role in cell gravisensing is attributed to the actin cytoskeleton which acts as a mediator in signaling reactions, including graviperception. Despite of increased attention to the actin cytoskeleton, major gaps in our understanding of its functioning in plant gravisensing still remain. To fill these gaps, we propose a novel approach focused on the investigation of actin involvement in the development of columella cells and cells in the transition zone of roots submitted to clinorotation. Both statocytes and cells in the transition zone represent the postmitotic cells which take origin in root meristems and are specified into graviperceptive (root cap) and gravireacting (transition zone) root tissues. The aim of the research was to investigate and compare the microfilament arrangements in root cap statocytes and peripheral root tissues (epidermis and cortex cells) in the transition zone and to find out how the actin cytoskeleton is involved in their specification under clinostat conditions. So far, our experiments have shown that under clinorotation the cytoplasmic microfilament network in the cortex cells in the transition zone is significantly enhanced. It is suggested that more abundant cytoplasmic microfilaments could strengthen the cortical actin cytoskeleton arranged parallel with the cortical microtubules, which are found to be partially disorganized in this area. Due to microtubule disorganization, the functioning of cellulose-synthesizing machinery and proper deposition of cell wall might be affected and could cause the alterations in the growth mode. But, in our case growth of the cells in the transition zone under clinorotation was rather stable. Due to our opinion, general stability of cell growth under clinorotation is promoted by mutual functional interrelation between actin and tubulin cytoskeletons. It is suggested that a strengthened cortical actin cytoskeleton restricts the cell growth instead of disorganized microtubules.     

Neuritogenesis: a model for space radiation effects on the central nervous system.

Pivotal to the astronauts' functional integrity and survival during long space flights are the strategies to deal with space radiations. The majority of the cellular studies in this area emphasize simple endpoints such as growth related events which, although useful to understand the nature of primary cell injury, have poor predictive value for extrapolation to more complex tissues such as the central nervous system (CNS). In order to assess the radiation damage on neural cell populations, we developed an in vitro model in which neuronal differentiation, neurite extension, and synaptogenesis occur under controlled conditions. The model exploits chick embryo neural explants to study the effects of radiations on neuritogenesis. In addition, neurobiological problems associated with long-term space flights are discussed.     

Local-area helioseismology as a diagnostic tool for solar variability

Dynamical and thermal variations of the internal structure of the Sun can affect the energy flow and result in variations in irradiance at the surface. Studying variations in the interior is crucial for understanding the mechanisms of the irradiance variations. “Global” helioseismology based on analysis of normal mode frequencies, has helped to reveal radial and latitudinal variations of the solar structure and dynamics associated with the solar cycle in the deep interior. A new technique, - “local-area” helioseismology or heliotomography, offers additional potentially important diagnostics by providing three-dimensional maps of the sound speed and flows in the upper convection zone. These diagnostics are based on inversion of travel times of acoustic waves which propagate between different points on the solar surface through the interior. The most significant variations in the thermodynamic structure found by this method are associated with sunspots and complexes of solar activity. The inversion results provide evidence for areas of higher sound speed beneath sunspot regions located at depths of 4–20 Mm, which may be due to accumulated heat or magnetic field concentrations. However, the physics of these structures is not yet understood. Heliotomography also provides information about large-scale stable longitudinal structures in the solar interior, which can be used in irradiance models. This new diagnostic tool for solar variability is currently under development. It will require both a substantial theoretical and modeling effort and high-resolution data to develop new capabilities for understanding mechanisms of solar variability.     

Relaxation of electron and proton radiation belts of the earth after strong magnetic storms

During strong magnetic storms in July and November of 2004 the fluxes of trapped particles (protons and electrons of MeV energies) in the Earth’s radiation belts have increased by orders of magnitude and then decreased remaining on an enhanced level for several months. These enhancements allowed us to study the processes of relaxation of the radiation belts. Measurements of energetic particles by low-altitude satellites Coronas-F and Servis-1 have shown that predictions of the theory about the rate of pitch-angle diffusion are not always correct, giving both overestimated and underestimated values for the lifetime of energetic particles.     

Multiple hypothesis track maintenance with possibly unresolved measurements

In surveillance problems dense clutter/dense target situations call for refined data association and tracking techniques. In addition, closely spaced targets may exist which are not resolved. This phenomenon has to be considered explicitly in the tracking algorithm. We concentrate on two targets which temporarily move in close formation and derive a generalization of MHT methods on the basis of a simple resolution model.     

The Detection Performance of the Siebert and Dicke-Fix CFAR Radar Detectors

The Siebert and the Dicke-fix CFAR radar detectors, used to maintain a constant false alarm rate (CFAR) in radar receivers under very similar circumstances, are considered. The Siebert detector represents the maximum-likelihood detection procedure for a signal in Gaussian noise of unknown power level, whereas the Dicke-fix makes use of a bandpass limiter to normalize the input and thus ensure a constant false alarm rate. The detection performance of the two detectors is determined and a comparison shows that over a wide range of parameters, the Dicke-fix introduces a loss which is approximately 1 B larger than for the Siebert detector.     

The observation of the galactic anticenter region by the balloon borne gamma-ray telescope Natalya-I

This paper presents the results of measurements of gamma radiation with energies above 5 MeV, from the galactic anticenter region. The balloon-borne gamma ray telescope “Natalya-I”, was launched on 6 November, 1980 from the Tata Institute of Fundamental Research's Balloon Facility (Hyderabad, India) and reached ceiling altitude of 35 km. The results on the accelerator calibration of the telescope, using a “tagged” gamma ray beam are also presented.