High phosphorylase activity is correlated with increased potato minituber formation and starch content during extended clinorotation.

The major purpose of these experiments were to investigate growth of potato storage organs and starch synthesis in minitubers at slow horizontal clinorotation (2 rpm), which partly mimics microgravity, and a secondary goal was to study the activity and localization of phosphorylase (EC 2.4.1.1) in storage parenchyma under these conditions. Miniplants of Solanum tuberosum L. (cv Adreta) were grown in culture for 30 days for both the vertical control and the horizontal clinorotation. During long-term clinorotation, an acceleration of minituber formation, and an increase of amyloplast number and size in storage parenchyma cells, as well as increased starch content, was observed in the minitubers. The differences among cytochemical reaction intensity, activity of phosphorylase, and carbohydrate content in storage parenchyma cells of minitubers grown in a horizontal clinostat were established by electron-cytochemical and biochemical methods. It is shown that high phosphorylase activity is correlated with increased starch content during extended clinorotation. The results demonstrate the increase in carbohydrate metabolism and possible accelerated growth of storage organs under the influence of microgravity, as mimicked by clinorotation; therefore, clinorotation can be used as a basis for future studies on mechanisms of starch synthesis under microgravity.     

The Earth's Magnetosphere Response to Solar Wind Events according to the INTERBALL Project Data

The results of studying the interaction of two types of the solar wind (magnetic clouds and solar wind of extremely low density) with the Earth's magnetosphere are discussed. This study is based of the INTERBALL space project measurements and on the other ground-based and space observations. For moderate variations of the solar wind and interplanetary magnetic field (IMF) parameters, the response of the magnetosphere is similar to its response to similar changes in the absence of magnetic clouds and depends on a previous history of IMF variations. Extremely large density variations on the interplanetary shocks, and on leading and trailing edges of the clouds result in a strong deformation of the magnetosphere, in large-scale motion of the geomagnetic tail, and in the development of magnetic substorms and storms. The important consequences of these processes are: (1) the observation of regions of the magnetosphere and its boundaries at great distances from the average location; (2) density and temperature variations in the outer regions of the magnetosphere; (3) multiple crossings of geomagnetic tail boundaries by a satellite; and (4) bursty fluxes of electrons and ions in the magnetotail, auroral region, and the polar cap. Several polar activations and substorms can develop during a single magnetic cloud arrival; a greater number of these events are accompanied, as a rule, by the development of a stronger magnetic storm. A gradual, but very strong, decrease of the solar wind density on May 10–12, 1999, did not cause noticeable change of geomagnetic indices, though it resulted in considerable expansion of the magnetosphere.     

Application of nonlinear filtering to navigation system designusing passive sensors

The problem of navigation system design for autonomous aircraft landing is addressed. New nonlinear filter structures are introduced to estimate the position and velocity of an aircraft with respect to a possibly moving landing site, such as a naval vessel, based on measurements provided by airborne vision and inertial sensors. By exploring the geometry of the navigation problem, the navigation filter dynamics are cast in the framework of linear parametrically varying systems (LPVs). Using this set-up, filter performance and stability are studied in an H_∞ setting by resorting to the theory of linear matrix inequalities (LMIs). The design of nonlinear, regionally stable filters to meet adequate H_∞ performance measures is thus converted into that of determining the feasibility of a related set of LMIs and finding a solution to them, if it exists. This is done by using-widely available numerical tools that borrow from convex optimization techniques. The mathematical framework that is required for integrated vision/inertial navigation system design is developed and a design example for an air vehicle landing on an aircraft carrier is detailed     

The storm-time ring current

In this paper I am reviewing recent advances and open disputes in the study of the terrestrial ring current, with emphasis on its storm-time dynamics. The ring current is carried by energetic charged particles flowing toroidally around the Earth, and creating a ring of westward electric current, centered at the equatorial plane and extending from geocentric distances of about 2 R _E to roughly 9 R _E. This current has a permanent existence due to the natural properties of charged particles in the geospace environment, yet its intensity is variable. It becomes more intense during global electromagnetic disturbances in the near-Earth space, which are known as space (or magnetic or geomagnetic) storms. Changes in this current are responsible for global decreases in the Earth's surface magnetic field, which is the defining feature of geomagnetic storms. The ring current is a critical element in understanding the onset and development of space weather disturbances in geospace. Ring current physics has long been driven by several paradigms, similarly to other disciplines of space physics: the solar origin paradigm, the substorm-driver paradigm, the large-scale symmetry paradigm, the charge-exchange decay paradigm. The paper addresses these paradigms through older and recent important investigations.     

Radiation biodosimetry: applications for spaceflight.

The multiparametric dosimetry system that we are developing for medical radiological defense applications could be adapted for spaceflight environments. The system complements the internationally accepted personnel dosimeters and cytogenetic analysis of chromosome aberrations, considered the best means of documenting radiation doses for health records. Our system consists of a portable hematology analyzer, molecular biodosimetry using nucleic acid and antigen-based diagnostic equipment, and a dose assessment management software application. A dry-capillary tube reagent-based centrifuge blood cell counter (QBC Autoread Plus, Becton [correction of Beckon] Dickinson Bioscience) measures peripheral blood lymphocytes and monocytes, which could determine radiation dose based on the kinetics of blood cell depletion. Molecular biomarkers for ionizing radiation exposure (gene expression changes, blood proteins) can be measured in real time using such diagnostic detection technologies as miniaturized nucleic acid sequences and antigen-based biosensors, but they require validation of dose-dependent targets and development of optimized protocols and analysis systems. The Biodosimetry Assessment Tool, a software application, calculates radiation dose based on a patient's physical signs and symptoms and blood cell count analysis. It also annotates location of personnel dosimeters, displays a summary of a patient's dosimetric information to healthcare professionals, and archives the data for further use. These radiation assessment diagnostic technologies can have dual-use applications supporting general medical-related care.     

Planetarium Inversum -- a space vision for Earth education.

In a planetarium, the visitor is sitting on Earth and looking into an imaginary space. The Planetarium Inversum is the opposite: visitors are sitting in a space station, looking down on Mother Earth. It is a scientifically-based information show with visitors involvement, its elements being partially virtual (Earth in space has to be projected with highest possible resolution) but also containing real structures, such as the visitors' Earth observatory with adjacent biological systems (plant cultures and other ecological life support components). Its main message concerns the limits and the vulnerability of our home planet, its uniqueness, beauty and above all, its irreplaceableness: Earth does not have an emergency exit. The Earth observatory is part of a ring shaped, rotating space station of the type designed by Wernher von Braun decades ago. Visitors are told that gravity is being substituted by centrifugal force. Both types of life support systems are being demonstrated--self regenerative life based ones and technical ones as a backup (solar electric splitting of water and chemical absorption of respiratory CO2).     

Development of a Digital Array Radar (DAR)

Twenty-first century littoral and open-sea missions present US Navy (USN) shipboard-radar systems with the challenge of detecting small targets in severe clutter and against multiple sources of interference. In Fiscal Year 2000 (FY00), the Office of Naval Research (ONR) sponsored a program to develop an active array radar that includes a digital beamforming (DBF) architecture. The DBF radar system has the potential for improved time-energy management, improved signal-to-clutter (S/C) ratios, improved reliability and reduced life-cycle costs. This paper summarizes the latest developments of the program during FY00     

Competition of the natural and manmade biotic cycles in the closed aquatic system.

This study addresses competition between the Paramecium bursaria and zoochlorella-endosymbiosis and the infusoria Paramecium caudatum in a closed aquatic system. The system is a natural model of a simple biotic cycle. P. bursaria consumes glucose and oxygen released by its zoochlorella and releases nitrogenous compounds and carbon dioxide necessary for algal photosynthesis. P. caudatum was fed on bacteria. It was shown that the infusoria P. bursaria united in one cycle with Chlorella had a higher competitive ability than P. caudatum. With any initial percentage of the infusoria in the mixed culture, the end portion of P. bursaria reached 90-99%, which was significantly higher than the end potion of the P. caudatum population. It is assumed that the sustenance expenditures of P. caudatum were greater than those of the endosymbiotic paramecium, i.e. the closing of the components into a biotic cycle leads to a decrease in sustenance expenditures.     

Population dynamics of transgenic microorganisms in the different microecosystem conditions.

The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap (r) Lux+) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human alpha 2-interferon Km (r) Inf+), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. Grant numbers: 99-04-96017, 25, 00-07-9011.