Heavy ion induced double strand breaks in bacteria and bacteriophages.

DNA damage induced by heavy ions in bacterial cells and bacteriophages such as Bacillus subtilis, E. coli and Bacteriophage T1 were investigated by analyzing the double strand breaks in the chromosomal DNA. This kind of lesion is considered as one of the main reasons for lethal events. To analyze double strand breaks in long molecules of DNA--up to some Mbp in length--the technique of pulse field agarose gel electrophoresis has been used. This allows the detection of one double strand break per genome. Cell lysis and DNA isolation were performed in small agarose blocks directly. This procedure secured minimum DNA destruction by shearing forces. After running a gel, the DNA was stained with ethidium bromide. The light intensity of ethidium bromide fluorescence for both the outcoming (running) DNA and the remaining intact DNA were measured by scanning. The mean number of double strand breaks was calculated by determining the quotient of these intensities. Strand break induction after heavy ion and X-ray irradiation was compared.     

Production of hydrocarbons and nitriles by electrical processes in Titan's atmosphere.

Although lightning has not been observed in Titan's atmosphere, the presence of methane rain in the troposphere suggests the possibility of electrical activity in the form of corona and/or lightning discharges. Here we examine the chemical effects of these electrical processes on a Titan simulated atmosphere composed of CH4 in N2 at various mixing ratios. Corona discharges were simulated in two different experimental arrays. For the detection of reactive intermediates we used a mass spectrometer to study the main positive ions arising by bombarding low-energy electrons from a hot filament into low-pressure methane. The final stable products, generated by applying a high voltage in a coaxial reactor with either positive or negative polarity, were separated and detected by gas chromatography-Fourier transform infrared spectroscopy and electron impact mass spectrometry (GC-FTIR-MS). Lightning discharges were simulated by a hot and dense plasma generated by a Nd-YAG laser and the final products were separated and detected by GC-FTIR-MS. Corona discharges produce linear and branched hydrocarbons as well as nitriles whereas lightning discharges generate mainly unsaturated hydrocarbons and nitriles. Lightning discharges are about 2 orders of magnitude more efficient in product formation than corona discharges.     

Geometric problem solving with strings and pins

Humans solve spatial and abstract problems more easily if these can be visualized and/or physically manipulated. We analyze the domain of geometric problem solving from a cognitive perspective and identify several levels of domain abstraction that interact in the problem solving process. We discuss the roles of physical manifestations of spatial configurations, their manipulation, and their perception for understanding problem solving processes. We propose an extension of the classical problem solving repertoire of constructive geometry to approach certain problems more directly than under the compass-and-straightedge paradigm. Specifically, we introduce strings and pins as helpful metaphors for a generalization of the constructive geometry approach. We present classical problems from spatial problem solving to illustrate the ‘strings and pins’ paradigm. Three case studies are discussed: strings-and-pins solutions to (i) the ellipse construction problem; (ii) the shortest path problem; and (iii) the angle trisection problem. Comparisons to formal solutions are drawn. Differences and similarities between the compass-and-straightedge paradigm and the strings-and-pins paradigm are analyzed. Features and limitations of constructive and depictive geometry as well as implications for computational approaches are discussed. The strings-and-pins domain is shown to be more general and less restrictive than the compass-and-straightedge domain.     

Temporal variation and asymmetry of sunspot and solar plage types from 1930 to 1936

Using the recently converted to digital format heliophysics catalogues of the Ebro Observatory published in the 1930s, we analyse simultaneously the temporal variation and asymmetry of two different solar structures located at different layers of the solar atmosphere: sunspots and solar plages. In particular, we do the research for all the types of sunspots and plages, including the daily and relative frequencies over the solar cycle. The data were catalogued using the sunspot Cortie classification and a solar plage classification scheme proposed by the Ebro Observatory, which group the phenomena by size and shape. For all types of both sunspots and plages, we observe a decrease in their frequency up to the end of solar cycle 16 and an increase over the beginning of solar cycle 17. Furthermore, we note that small sunspot groups are more likely to happen than bigger groups, although single big spots dominate near the solar minimum. The daily frequency of solar plage occurrences shows that there is not a dominance of compact or scattered solar plages. The North-South occurrence distribution of every type in both sunspots and solar plages shows an asymmetry during the solar cycle: in its declining phase, such asymmetry is directed to the north, while in the beginning of a new cycle is directed to the south.     

World Space Observatory-Ultraviolet: ISSIS,the imaging instrument

The Imaging and Slitless Spectroscopy Instrument (ISSIS) will be flown as part of the science instrumentation in the World Space Observatory-Ultraviolet (WSO-UV). ISSIS will be the first UV imager to operate in a high Earth orbit from a 2 m class space telescope. In this contribution, the science driving the ISSIS design and the main characteristics of this instrument are presented.     

Modeling gravity effects on water retention and gas transport characteristics in plant growth substrates

Growing plants to facilitate life in outer space, for example on the International Space Station (ISS) or at planned deep-space human outposts on the Moon or Mars, has received much attention with regard to NASA’s advanced life support system research. With the objective of in situ resource utilization to conserve energy and to limit transport costs, native materials mined on Moon or Mars are of primary interest for plant growth media in a future outpost, while terrestrial porous substrates with optimal growth media characteristics will be useful for onboard plant growth during space missions. Due to limited experimental opportunities and prohibitive costs, liquid and gas behavior in porous substrates under reduced gravity conditions has been less studied and hence remains poorly understood. Based on ground-based measurements, this study examined water retention, oxygen diffusivity and air permeability characteristics of six plant growth substrates for potential applications in space, including two terrestrial analogs for lunar and Martian soils and four particulate substrates widely used in reduced gravity experiments. To simulate reduced gravity water characteristics, the predictions for ground-based measurements (1 − g) were scaled to two reduced gravity conditions, Martian gravity (0.38 − g) and lunar gravity (0.16 − g), following the observations in previous reduced gravity studies. We described the observed gas diffusivity with a recently developed model combined with a new approach that estimates the gas percolation threshold based on the pore size distribution. The model successfully captured measured data for all investigated media and demonstrated the implications of the poorly-understood shift in gas percolation threshold with improved gas percolation in reduced gravity. Finally, using a substrate-structure parameter related to the gaseous phase, we adequately described the air permeability under reduced gravity conditions.     

Spin-axis pointing of a magnetically actuated spacecraft

Attitude regulation proves to be a challenging problem, when magnetic actuators alone are used as attitude effectors, since they do not provide three independent control torque components at each time instant. In this paper a rigorous proof of global exponential stability is derived for a magnetic control law that leads the satellite to a desired spin condition around a principal axis of inertia, pointing the spin axis toward a prescribed direction in the inertial frame. The technique is demonstrated by means of numerical simulation of a few example maneuvers. An extensive Monte Carlo simulation is performed for random initial conditions, in order to investigate the effect of changes in control law gains.     

Enrichment of the Hot Intracluster Medium: Observations

Four decades ago, the firm detection of an Fe-K emission feature in the X-ray spectrum of the Perseus cluster revealed the presence of iron in its hot intracluster medium (ICM). With more advanced missions successfully launched over the last 20 years, this discovery has been extended to many other metals and to the hot atmospheres of many other galaxy clusters, groups, and giant elliptical galaxies, as evidence that the elemental bricks of life—synthesized by stars and supernovae—are also found at the largest scales of the Universe. Because the ICM, emitting in X-rays, is in collisional ionisation equilibrium, its elemental abundances can in principle be accurately measured. These abundance measurements, in turn, are valuable to constrain the physics and environmental conditions of the Type Ia and core-collapse supernovae that exploded and enriched the ICM over the entire cluster volume. On the other hand, the spatial distribution of metals across the ICM constitutes a remarkable signature of the chemical history and evolution of clusters, groups, and ellipticals. Here, we summarise the most significant achievements in measuring elemental abundances in the ICM, from the very first attempts up to the era of XMM-Newton, Chandra, and Suzaku and the unprecedented results obtained by Hitomi. We also discuss the current systematic limitations of these measurements and how the future missions XRISM and Athena will further improve our current knowledge of the ICM enrichment.